MCDBIO165A Winter 2025 Midterm 1 Answer key University of Illinois, Chicago

MCDB165a Winter 2025 Midterm I

Question 1
What type of microscopy was used to take this image? (5 points)




a. Light microscopy with a Golgi stain
b. Fluorescence microscopy
c. Transmission electron microscopy
d. Scanning electron microscopy

2. In this image from the background video,

, What type of microscopy is shown in subfigure f and how do you know? (5
points)

a. Subfigure f is showing confocal microscopy. I know because I can see that
the center of the sphere is yellow while in c, with widefield fluorescence
microscopy, the sphere looks red.
b. Subfigure f is showing transmission electron microscopy. I know because
the resolution is much better than subfigure c.
c. Subfigure f is showing super resolution microscopy. I know because I
would only be able to see that the inside of the sphere is yellow if the
diffraction limit of light was surpassed.
d. Subfigure f is showing differential interference contrast microscopy. I
know because the sample has a 3-dimensional look that is not observed with
microscopy without DIC in subfigure c.

3. Which of the following is true about the confocal microscope shown (5
points)