Phlebotomy Chapters 8 & 9 Objectives Exam – Special Blood
Collection Procedures and Dermal Puncture Techniques
Complete Questions with A+ Graded Answers
List the equipment that should be available for venipucture.
1. needles
2. tubes
3. gloves
4. bandages
5. marking pens
6. needle holders
7. syringes with transfer device
8. tourniquets
9. winged infusion sets (butterflies)
10. collection tubes
11. antiseptic cleaning solution
12. gauze pads
Describe the purpose of a tourniquet, and list types that may be used to locate a vein.
The purpose is to prevent venous blood flow out of the arm causing the veins to bulge.
1. simple strip of latex
2. nonlatex made from synthetic rubber
3. blood pressure cuff
Differentiate between an antiseptic and a disinfectant, and list those that may be used for blood
collection.
Antiseptic is an agent used to clean living tissue and disinfectant is an agent used to clean a
surface other than living tissue.
1. 70 isopropyl alcohol
2. Povidone- iodine solution
3. chlorhexidine gluconate
Locate the bevel, shaft, hub, and point of a needle, and describe safety features that may be
included.
Bevel is slanted end of the needle, shaft is the outer portion of the needle, hub is at one end
that attaches to the syringe and point is located where the bevel is where it is formed. They may
use self-capping needles and winged collection set.
, Define needle gauge, and explain the use of different gauges.
Needle gauge is a diameter of the luman where it has sizes between 16 to 23. The 16 to 18 is
use to collect blood from doners for transfusion, 23 is used for collection from small, fragile
veins. 21 is use for routine adult collection.
Name the parts of a syringe, and describe how the syringe system differs from the evacuated
tube system.
It has a barrel, plunger and a needle. The syringe system does not automatically fill with blood
unlike evacuated tube system can. You have to pull back the plunger evenly, gently, and slowly
to withdraw blood.
Explan when a syringe system or winged infusion set (butterfly) is used in blood collection.
Syringe system is use when patients with fragile or small veins and the vacuum of the collection
tube is likely to collapse the vein. Wing infusion set (butterfly) is used for venipuncture on small
veins, such as those in the hand and in elderly or pediatric patients
Describe the proper use of the tube holder (needle adapter).
The proper use is to ensure a firm, stable connection between these two essential parts
Differtitate whole blood, serum, and plasma, and list at least one use for each.
1. Whole blood is blood collected and mixed with an anticoagulant so that it will not clot. It is
used for most hematology test
2. Serum is the fluid portion of blood that remains after clotting. It is used for chemistry and
immunology tests
3. Plasma is the fluid portion of blood, including fibrinogen and other clotting factors. It is used
for coagulation studies
Describe at least nine additives, including that mode of action and uses.
1. SPS to inhibit complement and phagocytosis and it uses blood culture to recover
microorganism causing blood infection
2. Sodium citrate to fill the tube completely to maintain the ratio of nine parts blood and it uses
coagulation tests
3. Antiglycolytic agent that perserves glucose and uses in glucose tolerance test
4. Buffered sodium citrate to fille the tube completly to maintain the ratio of four parts blood to
one part citrate and it uses sedimentation rate tests
5. Thrombin allows for a 5 minute clotting time and it is use for stat chemistry for patients on
anticoagulant therapy
6. Heparin is to prevent the release of small amounts of material that could contaminate the
Collection Procedures and Dermal Puncture Techniques
Complete Questions with A+ Graded Answers
List the equipment that should be available for venipucture.
1. needles
2. tubes
3. gloves
4. bandages
5. marking pens
6. needle holders
7. syringes with transfer device
8. tourniquets
9. winged infusion sets (butterflies)
10. collection tubes
11. antiseptic cleaning solution
12. gauze pads
Describe the purpose of a tourniquet, and list types that may be used to locate a vein.
The purpose is to prevent venous blood flow out of the arm causing the veins to bulge.
1. simple strip of latex
2. nonlatex made from synthetic rubber
3. blood pressure cuff
Differentiate between an antiseptic and a disinfectant, and list those that may be used for blood
collection.
Antiseptic is an agent used to clean living tissue and disinfectant is an agent used to clean a
surface other than living tissue.
1. 70 isopropyl alcohol
2. Povidone- iodine solution
3. chlorhexidine gluconate
Locate the bevel, shaft, hub, and point of a needle, and describe safety features that may be
included.
Bevel is slanted end of the needle, shaft is the outer portion of the needle, hub is at one end
that attaches to the syringe and point is located where the bevel is where it is formed. They may
use self-capping needles and winged collection set.
, Define needle gauge, and explain the use of different gauges.
Needle gauge is a diameter of the luman where it has sizes between 16 to 23. The 16 to 18 is
use to collect blood from doners for transfusion, 23 is used for collection from small, fragile
veins. 21 is use for routine adult collection.
Name the parts of a syringe, and describe how the syringe system differs from the evacuated
tube system.
It has a barrel, plunger and a needle. The syringe system does not automatically fill with blood
unlike evacuated tube system can. You have to pull back the plunger evenly, gently, and slowly
to withdraw blood.
Explan when a syringe system or winged infusion set (butterfly) is used in blood collection.
Syringe system is use when patients with fragile or small veins and the vacuum of the collection
tube is likely to collapse the vein. Wing infusion set (butterfly) is used for venipuncture on small
veins, such as those in the hand and in elderly or pediatric patients
Describe the proper use of the tube holder (needle adapter).
The proper use is to ensure a firm, stable connection between these two essential parts
Differtitate whole blood, serum, and plasma, and list at least one use for each.
1. Whole blood is blood collected and mixed with an anticoagulant so that it will not clot. It is
used for most hematology test
2. Serum is the fluid portion of blood that remains after clotting. It is used for chemistry and
immunology tests
3. Plasma is the fluid portion of blood, including fibrinogen and other clotting factors. It is used
for coagulation studies
Describe at least nine additives, including that mode of action and uses.
1. SPS to inhibit complement and phagocytosis and it uses blood culture to recover
microorganism causing blood infection
2. Sodium citrate to fill the tube completely to maintain the ratio of nine parts blood and it uses
coagulation tests
3. Antiglycolytic agent that perserves glucose and uses in glucose tolerance test
4. Buffered sodium citrate to fille the tube completly to maintain the ratio of four parts blood to
one part citrate and it uses sedimentation rate tests
5. Thrombin allows for a 5 minute clotting time and it is use for stat chemistry for patients on
anticoagulant therapy
6. Heparin is to prevent the release of small amounts of material that could contaminate the
