,TEST BANK FOR CLS570 BIO-ORGANIC CHEMISTRY THE USES OF SYNTHETICS OLIGONUCLEOTIDES NOTES WELL EXPLAINED STEP BY STEP WITH ILLUSTRATIONS LATEST 2026/2027

BIO OGRANIC CHEMISTRY CLS570



TEST BANK FOR CLS570
BIO-ORGANIC CHEMISTRY
THE USES OF SYNTHETICS
OLIGONUCLEOTIDES NOTES
WELL EXPLAINED STEP BY
STEP WITH ILLUSTRATIONS
LATEST 2026/2027

,BIO OGRANIC CHEMISTRY CLS570

Uses of Synthesized
Oligonucleotides


Chemically synthesized oligonucleotides have lot of
functions.

1- Single stranded hybridization oligonucleotide probes
(20- to 40mers) can be formulated by deducing the
codons from the amino acid sequence of a protein and
then used to screen a genomic library for the gene.

,BIO OGRANIC CHEMISTRY CLS570

Nucleotide Sequences
Deduced from Amino Acid
Sequences

, BIO OGRANIC CHEMISTRY CLS570
Example A shows one possible nucleotide
sequence for a three amino acid
sequence

Uses of Synthesized
Oligonucleotides
Since the actual codons representing a conserved amino
acid sequence are unknown because of codon
redundacy, a single arbitrary synthetic probe may not
contain sufficient complementary bases to produce
significant hybridization with a heterologus sequence.

For this reason, a set of mixed probes is often used to
screen a genomic library.