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BIOL 201 W2024 – Self
Assessment Quiz #1 (Jan 19, 2026)
latest updated questions and
answers(SCORED 99%)
Western x3 questions
Which of the following observations are TRUE about the activity of
glyceraldehyde-3dehydrogenase (GAPDH), the enzyme in glycolysis
that transfers two electrons from glyceraldehyde-3-phosphate to
NAD+ while making 1,3-bisphosphoglycerate? (choose all that apply)
1. It performs a reduction reaction
2. It performs an oxidation reaction
3. It is una ected by thermal energy
4. It uses both NAD+ and ATP as substrates
5. It performs an essentially irreversible reaction
Correct response: 1 and 2 (GAPDH oxidizes glyceraldehyde-3-
phosphate and reduces NAD+; the second phosphate added to form
the product comes from inorganic phosphate; thermal energy a ects
all molecules in the cytoplasm; this is not one of the 3 “essentially
irreversible” reactions in glycolysis)
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Imagine you have 3 proteins, X, Y, Z that have the following diusion coe
icients:
Protein X: D = 3.6 μm2/s; Protein Y: D = 8.3 μm2/s; Protein Z: D = 2.8
μm2/s
(a) Match the proteins with their likely size in kiloDaltons
1. 27 kDa
2. 84 kDa
3. 138 kDa
Correct response: X = 84 kDa; Y = 27 kDa; Z = 138 kDa (the di usion coe
icient is inversely proportional to protein size; smaller proteins move
faster)
(b) Which protein would you expect to move the “fastest” in the
cytoplasm assuming only di usion is at play?
4. X
5. Y
6. Z
Correct response: Y will move the “fastest” (has the highest di usion coe
icient)
(c) Which of the following do you think, on average, would cause the
greatest impediment to the movement of the “slowest” protein
through the cytoplasm?
7. Charge
8. Being hit by other di using molecules
9. E ective pore size
Correct response: E ective pore size – being surrounded by other
proteins leaving little space for something large to move
The enzyme fluorase is an enzyme found in some bioluminescent
algae. It catalyzes the oxidation of its substrate phluorin leading to the
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EMAIL:
BIOL 201 W2024 – Self
Assessment Quiz #1 (Jan 19, 2026)
latest updated questions and
answers(SCORED 99%)
Western x3 questions
Which of the following observations are TRUE about the activity of
glyceraldehyde-3dehydrogenase (GAPDH), the enzyme in glycolysis
that transfers two electrons from glyceraldehyde-3-phosphate to
NAD+ while making 1,3-bisphosphoglycerate? (choose all that apply)
1. It performs a reduction reaction
2. It performs an oxidation reaction
3. It is una ected by thermal energy
4. It uses both NAD+ and ATP as substrates
5. It performs an essentially irreversible reaction
Correct response: 1 and 2 (GAPDH oxidizes glyceraldehyde-3-
phosphate and reduces NAD+; the second phosphate added to form
the product comes from inorganic phosphate; thermal energy a ects
all molecules in the cytoplasm; this is not one of the 3 “essentially
irreversible” reactions in glycolysis)
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EMAIL:
,FOR MORE EXAMS
EMAIL:
Imagine you have 3 proteins, X, Y, Z that have the following diusion coe
icients:
Protein X: D = 3.6 μm2/s; Protein Y: D = 8.3 μm2/s; Protein Z: D = 2.8
μm2/s
(a) Match the proteins with their likely size in kiloDaltons
1. 27 kDa
2. 84 kDa
3. 138 kDa
Correct response: X = 84 kDa; Y = 27 kDa; Z = 138 kDa (the di usion coe
icient is inversely proportional to protein size; smaller proteins move
faster)
(b) Which protein would you expect to move the “fastest” in the
cytoplasm assuming only di usion is at play?
4. X
5. Y
6. Z
Correct response: Y will move the “fastest” (has the highest di usion coe
icient)
(c) Which of the following do you think, on average, would cause the
greatest impediment to the movement of the “slowest” protein
through the cytoplasm?
7. Charge
8. Being hit by other di using molecules
9. E ective pore size
Correct response: E ective pore size – being surrounded by other
proteins leaving little space for something large to move
The enzyme fluorase is an enzyme found in some bioluminescent
algae. It catalyzes the oxidation of its substrate phluorin leading to the
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EMAIL:
