1 for specific request mail
BIOCH 330 Final Exam - Translation Questions and
Correct Answers | Latest Update
What is meant by the overlapping code?
Ans: -the fact that you can start reading codons one base pair over and
get an entirely new reading frame
-for a maximum of 3 reading frames total
What was "cell sap"?
Assignment Expert
Ans: in vitro mixture of ribosomes, enzymes, and cell constituents from
lysed bacteria that permitted translation of proteins outside of the cell
Guru01 - Stuvia
-used to figure out the genetic code
What amino acid was produced with synthetic poly(U) sequences?
Poly(A)? Poly(C)? Poly(G)?
2026
Ans: poly(U): F
©
poly(C): P
poly(A): K
poly(G): none
-later on discovered that poly(G) produces tetraplexes that ribosomes
cannot bind to
What is special about the lysate from rabbit reticulocytes?
Ans: -it is competent for in vitro translation
Are amino acid codons conserved across species?
Ans: yes, all of them
What are the three stop codons?
, 2 for specific request mail
Ans: UAA
UGA
UAG
What is the start codon? What amino acid does it encode?
Ans: AUG, Met
What is meant by the genetic code being degenerate?
Ans: -multiple codons encode for the same amino acid
Assignment Expert
How many codons encode all 20 amino acids?
Guru01 - Stuvia
Ans: 61
Where is the difference in codons that encode for the same amino acid?
Ans: the third position
2026
-aka the 3' position
©
If a codon is 5'-AUC-3', what is the corresponding anticodon?
Ans: 5'-GAU-3'
How many tRNAs are there for all 20 amino acids?
Ans: about 32
What is wobble?
Ans: -allows some tRNAs to recognize more than one codon
-this means that there isn't one single specific tRNA for each codon
What is the base in nucleotide inosinate? What bases does it base pair
with? Where is it found?
, 3 for specific request mail
Ans: i. hypoxanthine
ii. U, C, and A
iii. found on the first (5') base of the anticodon of some tRNAs
What does the wobble of a tRNA allow for it in terms of dissocation from
the ribosome?
Ans: -it can rapidly dissociate from its codon during protein synthesis
What wobble pairings are there?
Assignment Expert
Ans: Let each base pair be the 5' base in the anticodon:
U: can bp with A, G
Guru01 - Stuvia
G: can bp with C, U
C: can bp with G, I
2026
A: can bp with U, I
I: can bp with A, C, U
©
What is the wobble hypothesis?
Ans: 1. the first two bases of the mRNA codon confer most of the tRNA
specificity
2. the first base of the anticodon determines the number of codons
recognized by the tRNA
What is substitution editing? What is insertion/deletion editing?
Ans: Substitution:
-chemically altering the structure of nucleotides
Ins/Del:
BIOCH 330 Final Exam - Translation Questions and
Correct Answers | Latest Update
What is meant by the overlapping code?
Ans: -the fact that you can start reading codons one base pair over and
get an entirely new reading frame
-for a maximum of 3 reading frames total
What was "cell sap"?
Assignment Expert
Ans: in vitro mixture of ribosomes, enzymes, and cell constituents from
lysed bacteria that permitted translation of proteins outside of the cell
Guru01 - Stuvia
-used to figure out the genetic code
What amino acid was produced with synthetic poly(U) sequences?
Poly(A)? Poly(C)? Poly(G)?
2026
Ans: poly(U): F
©
poly(C): P
poly(A): K
poly(G): none
-later on discovered that poly(G) produces tetraplexes that ribosomes
cannot bind to
What is special about the lysate from rabbit reticulocytes?
Ans: -it is competent for in vitro translation
Are amino acid codons conserved across species?
Ans: yes, all of them
What are the three stop codons?
, 2 for specific request mail
Ans: UAA
UGA
UAG
What is the start codon? What amino acid does it encode?
Ans: AUG, Met
What is meant by the genetic code being degenerate?
Ans: -multiple codons encode for the same amino acid
Assignment Expert
How many codons encode all 20 amino acids?
Guru01 - Stuvia
Ans: 61
Where is the difference in codons that encode for the same amino acid?
Ans: the third position
2026
-aka the 3' position
©
If a codon is 5'-AUC-3', what is the corresponding anticodon?
Ans: 5'-GAU-3'
How many tRNAs are there for all 20 amino acids?
Ans: about 32
What is wobble?
Ans: -allows some tRNAs to recognize more than one codon
-this means that there isn't one single specific tRNA for each codon
What is the base in nucleotide inosinate? What bases does it base pair
with? Where is it found?
, 3 for specific request mail
Ans: i. hypoxanthine
ii. U, C, and A
iii. found on the first (5') base of the anticodon of some tRNAs
What does the wobble of a tRNA allow for it in terms of dissocation from
the ribosome?
Ans: -it can rapidly dissociate from its codon during protein synthesis
What wobble pairings are there?
Assignment Expert
Ans: Let each base pair be the 5' base in the anticodon:
U: can bp with A, G
Guru01 - Stuvia
G: can bp with C, U
C: can bp with G, I
2026
A: can bp with U, I
I: can bp with A, C, U
©
What is the wobble hypothesis?
Ans: 1. the first two bases of the mRNA codon confer most of the tRNA
specificity
2. the first base of the anticodon determines the number of codons
recognized by the tRNA
What is substitution editing? What is insertion/deletion editing?
Ans: Substitution:
-chemically altering the structure of nucleotides
Ins/Del:
