Blood Bank ASCP MB 2026 || MOST RECENT EXAM
ACTUAL COMPLETE REAL EXAM QUESTIONS AND
CORRECT ANSWERS (VERIFIED ANSWERS)
ALREADY GRADED A+ | GUARANTEED SUCCESS!! |
NEWEST EXAM | JUST RELEASED!! LATEST UPDATE
2026-2027
Which termination process in prokaryotes uses a hairpin structure to slow or
stop transcription and is also known as intrinsic termination?
Rho-dependent termination or Rho-independent
termination Why?
Rho-independent termination
Called independent because it does not require any additional factors or
enzymes to
initiate termination of transcription. Instead, the RNA strand will fold onto
itself, creating a hairpin structure, which in turn slows the RNA polymerase
from moving along the
strand and ultimately stopping transcription.
,How many RNA polymerases do eukaryotes have vs. prokaryotes?
Eukaryotes = Three RNA Polymerases
1.) RNA Pol I
2.) RNA Pol II
3.) RNA Pol III
Prokaryotes = One RNA Polymerase
What is the absorptivity constant of
DNA? Of RNA?
DNA = 50ug/mL (for
dsDNA) RNA = 40ug/mL
What is the equation to find the concentration of DNA or RNA when the
absorbance reading and the dilution factor are given?
Concentration = (Absorbance reading) x (absorptivity constant DNA/RNA) x
(dilution factor)
A technologist uses the spectrophotometer to quantify the amount of DNA
extracted
from a blood specimen diluted 1:30. The absorbance reading at 260 nm was
found to be
2.545. The concentration of DNA in this extract is:
Absorbance x Absorptivity constant x Dilution factor
2.545 x 50ug/mL x 30 = 3817.5ug/mL
,Mutation in what gene is associated with Fragile X syndrome?
H63D
CFTR
FMR1
F5
FMR1 = Fragile X Mental Retardation 1
*H63D and HFE = Hemochromatosis
CFTR = Cystic Fibrosis Transmembrane Conductance
Regulator F5 = Factor V Leiden (Factor 5 Leiden)
Why is stringency important during hybridization techniques?
Because stringency determines the conditions that allow a probe and target to
bind optimally.
Probe and target will bind the best when stringency conditions have been
optimized
, Pyrosequencing????
1.) A method of DNA sequencing that relies on the detection of
pyrophosphate released upon nucleotide incorporation.
2.) Detects addition of a nucleotide to the end of a synthesized strand of
DNA by production of light
Faster and cheaper than Sanger method.
3.) DNA sequencing technology that is based on the generation and
detection of a pyrophosphate group liberated from a nucleotide
triphosphate
Most common file type for NGS?
FASTQ
(Next most common is SFF)
A mutation in the MTHFR gene causes what vitamin
deficiency(ies)? MTHFR = Methylenetetrahydrofolate
Reductase
Affects the way the body metabolizes folate (folic acid) and other B vitamins.
**Blood test looks for increased homocysteine in the blood. Genetic testing
can also be preformed. Autosomal recessive.
ACTUAL COMPLETE REAL EXAM QUESTIONS AND
CORRECT ANSWERS (VERIFIED ANSWERS)
ALREADY GRADED A+ | GUARANTEED SUCCESS!! |
NEWEST EXAM | JUST RELEASED!! LATEST UPDATE
2026-2027
Which termination process in prokaryotes uses a hairpin structure to slow or
stop transcription and is also known as intrinsic termination?
Rho-dependent termination or Rho-independent
termination Why?
Rho-independent termination
Called independent because it does not require any additional factors or
enzymes to
initiate termination of transcription. Instead, the RNA strand will fold onto
itself, creating a hairpin structure, which in turn slows the RNA polymerase
from moving along the
strand and ultimately stopping transcription.
,How many RNA polymerases do eukaryotes have vs. prokaryotes?
Eukaryotes = Three RNA Polymerases
1.) RNA Pol I
2.) RNA Pol II
3.) RNA Pol III
Prokaryotes = One RNA Polymerase
What is the absorptivity constant of
DNA? Of RNA?
DNA = 50ug/mL (for
dsDNA) RNA = 40ug/mL
What is the equation to find the concentration of DNA or RNA when the
absorbance reading and the dilution factor are given?
Concentration = (Absorbance reading) x (absorptivity constant DNA/RNA) x
(dilution factor)
A technologist uses the spectrophotometer to quantify the amount of DNA
extracted
from a blood specimen diluted 1:30. The absorbance reading at 260 nm was
found to be
2.545. The concentration of DNA in this extract is:
Absorbance x Absorptivity constant x Dilution factor
2.545 x 50ug/mL x 30 = 3817.5ug/mL
,Mutation in what gene is associated with Fragile X syndrome?
H63D
CFTR
FMR1
F5
FMR1 = Fragile X Mental Retardation 1
*H63D and HFE = Hemochromatosis
CFTR = Cystic Fibrosis Transmembrane Conductance
Regulator F5 = Factor V Leiden (Factor 5 Leiden)
Why is stringency important during hybridization techniques?
Because stringency determines the conditions that allow a probe and target to
bind optimally.
Probe and target will bind the best when stringency conditions have been
optimized
, Pyrosequencing????
1.) A method of DNA sequencing that relies on the detection of
pyrophosphate released upon nucleotide incorporation.
2.) Detects addition of a nucleotide to the end of a synthesized strand of
DNA by production of light
Faster and cheaper than Sanger method.
3.) DNA sequencing technology that is based on the generation and
detection of a pyrophosphate group liberated from a nucleotide
triphosphate
Most common file type for NGS?
FASTQ
(Next most common is SFF)
A mutation in the MTHFR gene causes what vitamin
deficiency(ies)? MTHFR = Methylenetetrahydrofolate
Reductase
Affects the way the body metabolizes folate (folic acid) and other B vitamins.
**Blood test looks for increased homocysteine in the blood. Genetic testing
can also be preformed. Autosomal recessive.
