C tr cAc d Cycle lanth path rceact on
step
Iyruvate decarboxylat on connects glycolys s toaerob c cell resp rat on bycreat ng amolecule
acetyl Coa that
can read lyenter the c tr cac dcycle
Step 1
Acetyl CoA transfers theacetyl group onto the fourcarbon oxaloacetate to from c trate
Catalyzed by c tratesynthase
CoA5 Coo
o
ooo CoA5 CHz
cuz
O L c ö 1
kHz 1 Ho c Coo H2O_ Ho c Coo
ı
CoA
Hz C ı
00cL caz Hz
1
00 00
aldd condensat on CoAcomplex hydrolys s react on c trate
C trate synthase sd nner that cons sts oftwo dent cal subun ts
Conformat onal change occurs
H s274 g v ng carbonyl oxygen an Ht
Th s st mulates the removal
ofahydrogen onfromthemethylgroup
by Asp 375
Th sleads tothe format on oftheenol molecule
The Ht ofH s320 sg ven tothe Carbonyl groupof oxaloacetate
The Tı bond oftheand nucleoph le attacktheact vatedoxaloacetate
Thec k ye COA complex s stab l zed v a electr c nteract ons w th the
catalyt c res dues
Inthe f nal step H s274 w llact asanHt donor tohelp hydrolyze
the h gh energy th oester bond
Step
coo
c00 öoc H ı
c H2O H c OH
H
ç H
c c OH C
H2O 1 öoc d H
00C çıtacoo H c H
H c H ı
coo
Coo c s Acon te
C trate sa trate somer
Two step process that s catalyzed açan tase
by an enzyme that uses an ron
sulfur complex tocarryout a dehydrat on react on fot owedby a hydrat onreact on
i i i i i i
i i
, Step
thef rst ox dat ve decarboxylat on react onofthe TCAcycle
Undergo ng
Catalyzed by sac trate dehydrat on and t cons stsoftwo processes
çoo
H c OH 00C o abstract on
ofa pa rofh gh
oc NADt k
c.tt A.ooa.d.l t napa µ IItgyd.IEms
atetormcaIah 9h
H c H
H cı H otolosuc nate
coo
co soc tro.tooxalosuc nate
ooo o
k
oocacı
00C c H
l Ht _ş H l H
c CO2
H cı H ı
H c H
00 ı
coo
Oxalosuc nate
d ketoglutarate
sac trate NAD d ketoglutarate NADH CO2 net react on
Step
oocacı corn
saç
H kH NAD CoASH NADH CO2
H d
ı
H H c H
H kıt
ca ı
coo
d ketoglutarate
Th s step s catalyzed bythe d wh ch cons sts
ketoglutarate dehydrogenase complex of
3 d fferent enzymes
Eı L ketoglutarate dehydrogenase Ith am ne pyrophosphate
2 Ez d hydrol poylsucc nyl transferase kl pa cac d
3 Ez d hydrol poye dehydrogenase FAD
substratelevel
Step sucünyecoa phosphorylat on
CoA sac synthetase
P co
GDP l
G H H k H GTP
c H
ı a d
ı H
A µ
g
co co succ nyl.ca Succ nate
step
Iyruvate decarboxylat on connects glycolys s toaerob c cell resp rat on bycreat ng amolecule
acetyl Coa that
can read lyenter the c tr cac dcycle
Step 1
Acetyl CoA transfers theacetyl group onto the fourcarbon oxaloacetate to from c trate
Catalyzed by c tratesynthase
CoA5 Coo
o
ooo CoA5 CHz
cuz
O L c ö 1
kHz 1 Ho c Coo H2O_ Ho c Coo
ı
CoA
Hz C ı
00cL caz Hz
1
00 00
aldd condensat on CoAcomplex hydrolys s react on c trate
C trate synthase sd nner that cons sts oftwo dent cal subun ts
Conformat onal change occurs
H s274 g v ng carbonyl oxygen an Ht
Th s st mulates the removal
ofahydrogen onfromthemethylgroup
by Asp 375
Th sleads tothe format on oftheenol molecule
The Ht ofH s320 sg ven tothe Carbonyl groupof oxaloacetate
The Tı bond oftheand nucleoph le attacktheact vatedoxaloacetate
Thec k ye COA complex s stab l zed v a electr c nteract ons w th the
catalyt c res dues
Inthe f nal step H s274 w llact asanHt donor tohelp hydrolyze
the h gh energy th oester bond
Step
coo
c00 öoc H ı
c H2O H c OH
H
ç H
c c OH C
H2O 1 öoc d H
00C çıtacoo H c H
H c H ı
coo
Coo c s Acon te
C trate sa trate somer
Two step process that s catalyzed açan tase
by an enzyme that uses an ron
sulfur complex tocarryout a dehydrat on react on fot owedby a hydrat onreact on
i i i i i i
i i
, Step
thef rst ox dat ve decarboxylat on react onofthe TCAcycle
Undergo ng
Catalyzed by sac trate dehydrat on and t cons stsoftwo processes
çoo
H c OH 00C o abstract on
ofa pa rofh gh
oc NADt k
c.tt A.ooa.d.l t napa µ IItgyd.IEms
atetormcaIah 9h
H c H
H cı H otolosuc nate
coo
co soc tro.tooxalosuc nate
ooo o
k
oocacı
00C c H
l Ht _ş H l H
c CO2
H cı H ı
H c H
00 ı
coo
Oxalosuc nate
d ketoglutarate
sac trate NAD d ketoglutarate NADH CO2 net react on
Step
oocacı corn
saç
H kH NAD CoASH NADH CO2
H d
ı
H H c H
H kıt
ca ı
coo
d ketoglutarate
Th s step s catalyzed bythe d wh ch cons sts
ketoglutarate dehydrogenase complex of
3 d fferent enzymes
Eı L ketoglutarate dehydrogenase Ith am ne pyrophosphate
2 Ez d hydrol poylsucc nyl transferase kl pa cac d
3 Ez d hydrol poye dehydrogenase FAD
substratelevel
Step sucünyecoa phosphorylat on
CoA sac synthetase
P co
GDP l
G H H k H GTP
c H
ı a d
ı H
A µ
g
co co succ nyl.ca Succ nate
