LAB PRACTICAL BIOS 242 - CHAMBERLAIN
Study online at https://quizlet.com/_ciploe
1. what test is performed to test microbial sensitivity to different antibiotics?-
: kirby beuer test
2. what are the three shapes of bacteria?: cocci, bacilli, spirilla
3. what are the sterilizing condition in a steam autoclave?: 121 celsius at 15 psi for 15
min
4. what part of the microscope is used to adjust the amount of light entering
the sample?: aperture
5. microbiology is the study of microorganisms that are best defined as: too small
to be seen with the unaided eye
6. inflammation and fever are part of which line of defense: second line of defense
7. what are the four major steps of gram staining: crystal violet, grams iodine solution,
alcohol, safranin
8. what is the reaction/color of gram negative bacteria when crystal violet is
applied?: purple
9. what is the reaction/color of gram negative bacteria when gram iodine is
applied?: dye fixer
10. what is the reaction/color of gram negative bacteria when alcohol is ap-
plied: decolorize, clear
11. what is the reaction/color of gram negative bacteria when safranin is ap-
plied?: pink
12. the bacterial cell wall that has thick peptidolgycan with small amounts of
teichoic acid and lipoteichoic acid will be classified as: gram positive
13. what are the three specific safety precautions we take in microbiology
labs: wear gloves, wipe down everything, no drinks/food
14. why are the bacteria samples stored in the fridge?: decrease bacterial growth
15. as a result of gram staining, the gram positive cells will look ___, and the
gram negative cells will look ___: purple, pink
16. the microbial cultures plates generated in the science lab are discarded
in: biohazard bag
17. stains are used viewing samples under microscope to increase: resolution and
contrast
18. s epidermidis is: gram positive, cocci shaped bacteria living as integumentary flora
1/2
Study online at https://quizlet.com/_ciploe
1. what test is performed to test microbial sensitivity to different antibiotics?-
: kirby beuer test
2. what are the three shapes of bacteria?: cocci, bacilli, spirilla
3. what are the sterilizing condition in a steam autoclave?: 121 celsius at 15 psi for 15
min
4. what part of the microscope is used to adjust the amount of light entering
the sample?: aperture
5. microbiology is the study of microorganisms that are best defined as: too small
to be seen with the unaided eye
6. inflammation and fever are part of which line of defense: second line of defense
7. what are the four major steps of gram staining: crystal violet, grams iodine solution,
alcohol, safranin
8. what is the reaction/color of gram negative bacteria when crystal violet is
applied?: purple
9. what is the reaction/color of gram negative bacteria when gram iodine is
applied?: dye fixer
10. what is the reaction/color of gram negative bacteria when alcohol is ap-
plied: decolorize, clear
11. what is the reaction/color of gram negative bacteria when safranin is ap-
plied?: pink
12. the bacterial cell wall that has thick peptidolgycan with small amounts of
teichoic acid and lipoteichoic acid will be classified as: gram positive
13. what are the three specific safety precautions we take in microbiology
labs: wear gloves, wipe down everything, no drinks/food
14. why are the bacteria samples stored in the fridge?: decrease bacterial growth
15. as a result of gram staining, the gram positive cells will look ___, and the
gram negative cells will look ___: purple, pink
16. the microbial cultures plates generated in the science lab are discarded
in: biohazard bag
17. stains are used viewing samples under microscope to increase: resolution and
contrast
18. s epidermidis is: gram positive, cocci shaped bacteria living as integumentary flora
1/2
