CHT EXAM COMPLETE STUDY GUIDE QUESTIONS AND ANSWERS UPDATED 2026

CHT EXAM COMPLETE STUDY
GUIDE QUESTIONS AND ANSWERS
UPDATED 2026
ItAisApossibleAtoAconvertAtheAplasma
intoAserumAbyArecalcifyingAtheAplasmaAandAallowingAcoagulationAtoAoccur.A-
AAnswerACalciumAisAaddedAtoAtheAplasmaAtoAformAaAcomplexAwithAtheAcitrateApresentAi
nAtheAanti-coagulant.AThisArestoresAaAnormal
ionizedAcalciumAlevelAallowingAclottingAtoAoccur.


PolyspecificAantiserum
canAbe______AwithAlymphocytesAtoAdetermineAifAthereAisAaAsingleAantibodyAorAmultiple
antibodies.A-AAnswerAabsorbed


AnotherAapplicationAofAlymphocyte
absorptionAisAtoAuseAtheApatient'sAownAcellsAtoAremove_____.AAbsorptionAcanAbeAperforme
d
atAcoldAorAwarmAtemperaturesAdependingAonAtheAtypeAofAantibody.A-
AAnswerAautoantibody


_________AareAtypically
coldAreactingA(4oC)Aand________areAtypicallyAwarmAreactingA(22-37oC).A-
AAnswerAAutoantibodies
ABAcellAantibodies


PatientAserumAmayAcontainAautoantibodiesAand/orAIgMAantibodiesAthatAmayAinterfereAtheAb
indingAof
anti-
HLAAIgGAantibodiesAtoAtheAantigen.AInAcomplementAdependentAassaysA(CDC),AIgMAantibodies
canAactivateAcomplement;ApotentiallyAleadingAtoA___________.A-
AAnswerAfalseApositiveAreactivity.


DTTA-AAnswerAdithiothreitol,AalsoAknownAasACleland'sAReagent

,DTTAinactivatesAIgMAmoleculesAbyAcleavingAtheA_________AinAthe
pentamerAstructure.A-AAnswerAdisulphideAbonds


HeatAinactivationAworksAbyAcleavingAtheAdisulphideAbondsAinAthe
pentamerAstructure,AbutAisAlessAspecificAthanA_____.A-AAnswerADTT.


ALAA-AAnswerAtherapeuticAantilymphocyte
antibodies


ThymoglobulinA-AAnswerApurified,Apasteurized,AgammaAimmuneAglobulin,
obtainedAbyAimmunizationAofArabbitsAwithAhumanAthymocytes


OKT3A-AAnswerAaAmurineAIgGAmonoclonalAantibodyAdirectedAagainstACD3


IfAtheseAtherapeuticAantilymphocyte
antibodiesAareApresentAinAtheApatientAserum,AtheyAcanAcause_______AreactivityAin
TAcellAcytotoxicAassays.A-AAnswerAfalseApositive


ALAAremovalAisAaccomplishedAbyAabsorptionAusingA_____coatedAwithAanti-rabbitAorAanti-
mouseAimmunoglobulinAforAThymoblobulinAorAOKT3,Arespectively.A-
AAnswerAmagneticAbeads


serumApreparationsA-AAnswerA1.ARecalcificiationAofAplasma
2.AAdsorptionAwithAlymphocytes
3.AInactivationAofAIgMAantibodies
a.ADTTAtreatment
b.AHeatAInactivation
4.ADepletionAofAtherapeuticAanti-lymphocyteAantibodiesAfromAserum


WhyAisAcalciumAaddedAtoAplasmaAtoAconvertAitAtoAserum?A-
AAnswerACalciumAisAaddedAtoAtheAplasmaAtoAcombineAwithAtheAcitrateApresentAinAtheAan
ticoagulant.

,ThisArestoresAaAnormalAionizedAcalciumAlevelAandAallowsAclottingAtoAoccur.


AtAwhatAtemperatureAshouldAserumAabsorptionAwithAlymphocytesAbeAperformed?A-
AAnswerATypically,Aauto-
antibodiesAareAcoldAreactingAandAshouldAbeAabsorbedAatA4oCAwhileAalloantibodies
areAwarmAreactingAandAshouldAbeAabsorbedAatA37oC.


DTTAtreatmentAisAmoreAspecificAthanAheatAtreatmentAin
removingAIgMAantibodies.AHowever,ADTTAcanAalsoAaffectAtheA_______AinAIgGAantibodies.A-
AAnswerAdisulfideAbonds


WhatAhappensAifAweAdon'tAremoveAOKT3ApriorAtoAcytotoxicityAassays?A-
AAnswerAOKT3AisAaAmonoclonalAantibodyAdirectedAagainstAtheACD3Amolecule.ATherefore,At
he
bindingAofAOKT3AtoAanyACD3+AcellAwillAactivateAcomplementAandAlyseAtheAtargetAcell.AThis
Aresults
inAfalselyApositiveAreactivity.


LymphocytesAhaveAaAdensityAof_______A-AAnswerA~1.077Ag/ml.


CellsAwithAaAhigherAdensityAthanAFHA-AAnswerAredAcellsAandAgranulocytes


cellsAofAaAlowerAdensityAthanAlymphocytesA-AAnswerAplatelets


isolationAofAcellsAfromAlymphAnodesAorAspleenAisAnormallyAfastAandAsimpleAandAleadsAtoA
greaterA_____AandA______A-AAnswerAcellAyieldA
viability.


ObtainingAlymphocytesAfromAspleenAwillArequire
anAadditionalAFHAstepAalongAwithApurificationAstepsAdueAtoAaAlargeAnumberAofAcontaminati
ngAcells,
suchAasA_______A,______AandA_______.A-AAnswerAmacrophages
erythrocytes
platelets.

, intrinsicAcellApropertiesA-AAnswerAcellAsizeAand
granularityAofAtheAcell.


OneAmethodAofAlymphocyteAisolationA(HP)AutilizesAanAintrinsicAcellAproperty,A__________,A
byAusingAa
densityAgradientAmedium.A-AAnswerAcellAdensity


extrinsicAcellApropertiesA-AAnswerAcellAsurfaceAmarker,AphagocyticAcapabilitiesAand
adherenceAcapabilities.


ImmunomagneticAbeadAseparationsAuseAtheAextrinisicApropertyAofA__________AasAthe
isolationAtarget.A-AAnswerAcellAsurfaceAmarkers


NylonAwoolAisolationAincorporatesAtheAextrinsicApropertyAofA___________A-
AAnswerAadherenceAforABAcells.


NylonAwoolAisolationAprocedure:A-
AAnswerALymphocytesAareAobtainedAandAaddedAtoAaAcolumnApackedAwithAnylonAwool.ABA
cellsAwillAadhereAtoAtheAnylonAwoolAatAanAoptimalAtemperatureAofA37oAC.ATheAnon-
adherentATAcellsAcanAbeAcollectedAbyAwashingAtheAcolumnAseveralAtimesAwithAwarmAmedia.
ATheABAcellsAcanAthenAbeAelutedAby
plungingAcoldAmediaAthroughAtheAcolumn.


DeadAcellsAinAaAflowAcytometryAassayAcanAleadAto
____________AofAmonoclonalAantibodiesAand/orAfluorochromes.A-
AAnswerAnonspecificAbinding


VariousAcellApurificationAmethodsAareA-
AAnswerAPercollAandAThrombin:AremoveAgranulocytes,Aplatelets,AandAdeadAcells


Lympho-
kwik:AisolateAspecificApopulationsA(TA&/orABAlymphocytes,AlymphocytesAandAmonocytes)