Histotechnology HTL Exam -
Comprehensive Review Questions
and Answers Updated 2026
AlcianABlueAdemonstratesA-AAnswer✓AAcidAmucins
ATPaseAEnymeAHistochemistryAdemonstratesA-AAnswer✓AATPaseAinAmuscles
Bielschowsky'sASilverATechniqueAdemonatratesA-AAnswer✓ADendrites,Aaxons,Aneurofibrils
BrownA&ABrennAgramAstainAdemonstratesA-AAnswer✓Abacteria
CongoAredAdemonstratesA-AAnswer✓Aamyloid
CresylAEchtAVioletAdemonstratesA-AAnswer✓ANisslAsubstance
GeimsaAstainAdemonstratesA-AAnswer✓AmastAcellsAorAH.Apylori
GomoriABurtnerAdemonstratesA-AAnswer✓Amelanin
Gomori'sAAldehydeAFuchsin
demonstratesA-AAnswer✓AelasticAandAmastAcells
GordonA&ASweet'sAdemonstratesA-AAnswer✓Areticulin
Grocott'sAMethenamineASilverAdemonstratesA-AAnswer✓AfungiA(eg.APneumocystis)
HematoxylinA&AEosinAshowsA-AAnswer✓AgeneralAstain
,ImmunohistotochemistryA-AAnswer✓AAnythingAanAantibodyAcanAbeAmadeAfor
LuxolAfastAblueAstainAshowsA-AAnswer✓Amyelin
ToluidineAblueAstainAshowsA-AAnswer✓AmastAcellsAorAH.Apylory
Masson'sATrichromeAshowsA-AAnswer✓Acollagen
OilARedAOAtechniqueAshowsA-AAnswer✓AneutralAfatsA(simpleAfats)
PapanicolaouAtechniqueAshowsA-AAnswer✓AcytologyAsmears
PeriodicAAcidASchiff'sAshowsA-AAnswer✓AneutralAmucin,Aglycogen
Perl'sAPrussianABlueAshowsA-AAnswer✓AHemosiderin
Southgate'sAmucicarmineA-AAnswer✓AepithelialAacidAmucins
Verhoeff'sAVanAGiesonA-AAnswer✓Aelastic
VanAKossaAshowsA-AAnswer✓Acalcium
ZiehlANeelsenAshowsA-AAnswer✓AAcidAfastAbacilliA(T.B.)
NucleiAinAGomori'sAAldehydeAFuchsinAtechniqueA-AAnswer✓AyellowA-
AnoAspecificAstainAforAnuclei
MastAcellsAinAaAGomori'sAAldehydeAFuchsinAtechniqueA-AAnswer✓ApurpleA-
AGAFAnotAspecificAforAelastic
,GramApositiveAbacteriaAifAover-differentiatedAafterAcrystalAvioletA-AAnswer✓Ared
NucleiAinAtheABrownAandABrennAGramAstainAifAoverAdifferentiatedAwithApicricAacid/acetoneA
-AAnswer✓Ayellow
FungiAinAtheAPeriodicAAcidASchiffA(PAS)AtechniqueA-AAnswer✓AmagentaA-
AchitinAbindsAwithASchiffs
FungiAinAtheAGrocott'sAMethenamineASilverAtechniqueA-AAnswer✓AblackA-
AsilverAimpregnation
NeutralAmucinsAinAaAPASAtechniqueAifApotassiumApermanganateAisAusedAasAtheAoxidizingAa
gentA-AAnswer✓AlightApinkA-AcolourAofAbackgroundA-
ACHOAoveroxidizedAfromAaldehydesAtoAacidAstate
CytoplasmAinAanAH&EAifAoverAdifferentiatedAwithAacidAalcoholA-AAnswer✓ApinkA-
AonlyAaffectsAnuclei
EpithelialAacidAmucinsAinAtheASouthgate'sAmucicarmineAtechniqueA-AAnswer✓Ared
CytoplasmAinAanAH&EAifAtheAblueingAagentAisAnotAproperlyAremovedA-
AAnswer✓AveryApaleApink
NucleiAinAtheACongoARedAtechniqueA-AAnswer✓AblueA-
AstainedAwithAaluminumAhematoxylin
CalciumAinAtheAVonAKossaAtechniqueA-AAnswer✓AblackA-AsilverAimpregnation
Glycogen,AafterAdiastaseAinAtheAPeriodicAAcidASchiffAtechniqueA-AAnswer✓ApaleApinkA-
AbackgroundAcolour
MycobacteriumAafterAZiehlANeelsenAtechniqueA-AAnswer✓AredA-
AAFBAstainAwithAcarbolAfuchsin
, MycobacteriumAafterAZiehlANeelsenAtechniqueA-AAnswer✓AredA-
AAFBAstainAwithAcarbolAfuchsin
StreptococcusAafterAZiehlANeelsenAtechniqueA-AAnswer✓AblueA-AcolourAofAbackground
NeutralAlipidsAforAOilARedAOAcutAonAaAcryostatA-AAnswer✓AredAtoAorange
NeutralAlipidsAforAOilARedAOAcutAfromAwaxAblockA-AAnswer✓AnotAdemonstratedA-
Aremoved
HemosiderinAafterAaAPerl'sAPrussianABlueA-AAnswer✓Ablue
NucleiAafterAMasson'sAtrichromeAusingAanAaluminumAHematoxylinA-AAnswer✓AredA-
AsameAasAcytoplasm
FineAelasticAfibresAinAVerhoeff'sAVanAGiesonA(VVG)AafterAleavingAinAVanAGiesonAreagentAto
oAlongA-AAnswer✓AnotAblack/notAdemonstratedA-AironAhematoxylinAremovedAbyAtheAacid
CytoplasmAifAunderdifferentiatedAinAtheAVVGAtechniqueA-AAnswer✓AgreyAtoAblack
ReticulinAfibresAifAironAalumAisAskippedAinAtheAGordonAandASweet'sA-
AAnswer✓AnotAdemonstrated
MelaninAifApotassiumApermanganateAwasAusedAasAanAoxidizingAagentAinAtheAGomoriABurtn
erAtechniqueA-AAnswer✓AnotAdemonstratedA-Aremoved
MelaninAinAtheALuxolAfastAblueAifAoverdifferentiatedAwithAlithiumAcarbonateA-
AAnswer✓Apink
NisslAsubstanceAusingAtheACresylAechtAvioletAtechniqueA-AAnswer✓Aviolet
Comprehensive Review Questions
and Answers Updated 2026
AlcianABlueAdemonstratesA-AAnswer✓AAcidAmucins
ATPaseAEnymeAHistochemistryAdemonstratesA-AAnswer✓AATPaseAinAmuscles
Bielschowsky'sASilverATechniqueAdemonatratesA-AAnswer✓ADendrites,Aaxons,Aneurofibrils
BrownA&ABrennAgramAstainAdemonstratesA-AAnswer✓Abacteria
CongoAredAdemonstratesA-AAnswer✓Aamyloid
CresylAEchtAVioletAdemonstratesA-AAnswer✓ANisslAsubstance
GeimsaAstainAdemonstratesA-AAnswer✓AmastAcellsAorAH.Apylori
GomoriABurtnerAdemonstratesA-AAnswer✓Amelanin
Gomori'sAAldehydeAFuchsin
demonstratesA-AAnswer✓AelasticAandAmastAcells
GordonA&ASweet'sAdemonstratesA-AAnswer✓Areticulin
Grocott'sAMethenamineASilverAdemonstratesA-AAnswer✓AfungiA(eg.APneumocystis)
HematoxylinA&AEosinAshowsA-AAnswer✓AgeneralAstain
,ImmunohistotochemistryA-AAnswer✓AAnythingAanAantibodyAcanAbeAmadeAfor
LuxolAfastAblueAstainAshowsA-AAnswer✓Amyelin
ToluidineAblueAstainAshowsA-AAnswer✓AmastAcellsAorAH.Apylory
Masson'sATrichromeAshowsA-AAnswer✓Acollagen
OilARedAOAtechniqueAshowsA-AAnswer✓AneutralAfatsA(simpleAfats)
PapanicolaouAtechniqueAshowsA-AAnswer✓AcytologyAsmears
PeriodicAAcidASchiff'sAshowsA-AAnswer✓AneutralAmucin,Aglycogen
Perl'sAPrussianABlueAshowsA-AAnswer✓AHemosiderin
Southgate'sAmucicarmineA-AAnswer✓AepithelialAacidAmucins
Verhoeff'sAVanAGiesonA-AAnswer✓Aelastic
VanAKossaAshowsA-AAnswer✓Acalcium
ZiehlANeelsenAshowsA-AAnswer✓AAcidAfastAbacilliA(T.B.)
NucleiAinAGomori'sAAldehydeAFuchsinAtechniqueA-AAnswer✓AyellowA-
AnoAspecificAstainAforAnuclei
MastAcellsAinAaAGomori'sAAldehydeAFuchsinAtechniqueA-AAnswer✓ApurpleA-
AGAFAnotAspecificAforAelastic
,GramApositiveAbacteriaAifAover-differentiatedAafterAcrystalAvioletA-AAnswer✓Ared
NucleiAinAtheABrownAandABrennAGramAstainAifAoverAdifferentiatedAwithApicricAacid/acetoneA
-AAnswer✓Ayellow
FungiAinAtheAPeriodicAAcidASchiffA(PAS)AtechniqueA-AAnswer✓AmagentaA-
AchitinAbindsAwithASchiffs
FungiAinAtheAGrocott'sAMethenamineASilverAtechniqueA-AAnswer✓AblackA-
AsilverAimpregnation
NeutralAmucinsAinAaAPASAtechniqueAifApotassiumApermanganateAisAusedAasAtheAoxidizingAa
gentA-AAnswer✓AlightApinkA-AcolourAofAbackgroundA-
ACHOAoveroxidizedAfromAaldehydesAtoAacidAstate
CytoplasmAinAanAH&EAifAoverAdifferentiatedAwithAacidAalcoholA-AAnswer✓ApinkA-
AonlyAaffectsAnuclei
EpithelialAacidAmucinsAinAtheASouthgate'sAmucicarmineAtechniqueA-AAnswer✓Ared
CytoplasmAinAanAH&EAifAtheAblueingAagentAisAnotAproperlyAremovedA-
AAnswer✓AveryApaleApink
NucleiAinAtheACongoARedAtechniqueA-AAnswer✓AblueA-
AstainedAwithAaluminumAhematoxylin
CalciumAinAtheAVonAKossaAtechniqueA-AAnswer✓AblackA-AsilverAimpregnation
Glycogen,AafterAdiastaseAinAtheAPeriodicAAcidASchiffAtechniqueA-AAnswer✓ApaleApinkA-
AbackgroundAcolour
MycobacteriumAafterAZiehlANeelsenAtechniqueA-AAnswer✓AredA-
AAFBAstainAwithAcarbolAfuchsin
, MycobacteriumAafterAZiehlANeelsenAtechniqueA-AAnswer✓AredA-
AAFBAstainAwithAcarbolAfuchsin
StreptococcusAafterAZiehlANeelsenAtechniqueA-AAnswer✓AblueA-AcolourAofAbackground
NeutralAlipidsAforAOilARedAOAcutAonAaAcryostatA-AAnswer✓AredAtoAorange
NeutralAlipidsAforAOilARedAOAcutAfromAwaxAblockA-AAnswer✓AnotAdemonstratedA-
Aremoved
HemosiderinAafterAaAPerl'sAPrussianABlueA-AAnswer✓Ablue
NucleiAafterAMasson'sAtrichromeAusingAanAaluminumAHematoxylinA-AAnswer✓AredA-
AsameAasAcytoplasm
FineAelasticAfibresAinAVerhoeff'sAVanAGiesonA(VVG)AafterAleavingAinAVanAGiesonAreagentAto
oAlongA-AAnswer✓AnotAblack/notAdemonstratedA-AironAhematoxylinAremovedAbyAtheAacid
CytoplasmAifAunderdifferentiatedAinAtheAVVGAtechniqueA-AAnswer✓AgreyAtoAblack
ReticulinAfibresAifAironAalumAisAskippedAinAtheAGordonAandASweet'sA-
AAnswer✓AnotAdemonstrated
MelaninAifApotassiumApermanganateAwasAusedAasAanAoxidizingAagentAinAtheAGomoriABurtn
erAtechniqueA-AAnswer✓AnotAdemonstratedA-Aremoved
MelaninAinAtheALuxolAfastAblueAifAoverdifferentiatedAwithAlithiumAcarbonateA-
AAnswer✓Apink
NisslAsubstanceAusingAtheACresylAechtAvioletAtechniqueA-AAnswer✓Aviolet
