BIOCHEMIST 3023 Biochem Exam 2 all in all/ Biochem Exam 2 - 2020

BIOCHEMIST 3023 Biochem Exam 2 all in all/ Biochem Exam 2 - 2020 Biochem Exam 2: 1. Which enzyme is considered part of TCA and ETC? a. Malate dehydrogenase b. Citrate synthase c. Isocitrate dehydrogenase d. NADH- ubiquinone oxidoreductase e. FADH2- ubiquinone oxidoreductase 2. A solution at pH 7 contains a weak acid, HA. The pKa of the acid is 6.5 what is the ratio of [A-/HA]? Answer: 3.16:1 3. In mitochondria, what is the expected pH of the matrix compared to the inter-membrane space? a. More acidic b. The same c. More neutral d. More basic e. Greater proton concentration 4. Compound C gives electrons to compound D, then compound D is a(n) and compound C is a. Oxidant, reductant b. Reductant, oxidant c. Oxidized, reduced d. Reduced, oxidized 5. When compound C gives electrons to Compound D, then Compound D was and Compound C was a. Oxidant, reductant b. Reductant, oxidant c. Oxidized, reduced d. Reduced, oxidized 6. What provides the energy to the ATP synthase for the formation of ATP? a. Electron flow b. Proton flow c. GTP d. Phosphoryl transfer e. Equilibrium shift (km change) 7. What do cristae structures in the mitochondria provide? a. Pores for the movement of proteins b. These are the proteins found in the outer membrane c. These proteins seal the inner membrane d. These structures increase surface area of the inner membrane 8. (True / False) The amino acid T at pH 7.4 has two charged groups but is actually a net zero charge. 9. 10. Which species is the reductant for the proposed ETC? a. Ickygreenol b. Barsoom c. Barstool d. Ickygreenone 11. Which species is the oxidant for the proposed ETC? a. Ickygreenol b. Barsoom c. Barstool d. Ickygreenone 12. Which series of voltages represents the correct voltages to trace the electron flow for the free calculation? a. -0.5 and -1.25 V b. +0.5 and -1.25 V c. -0.5 and +1.25 d. +0.5 and +1.25 V 13. What is the free energy gained from the electron transport chain presented? a. -34.5 kcal/mol b. +80.5 kcal/mol c. +34.5 kcal/mol d. -80.5 kcal/mol e. It cannot be determined from the information given 14. From one run of the proposed electron transport chain, how may ATP equivalents to be generated? a. 3 b. 4 c. 5 d. 6 e. 7 15.(True/False) 16. Material in figure 4? 17. What is the rate-limiting enzyme