Rh null - ANSWER Unusual phenotype that has no Rh antigens (D, C, E, c, or e), associated
with hemolytic anemia.
Rh antibodies - ANSWER Clinically significant antibodies (IgG) that may agglutinate at 37C
but will agglutinate in the AHG phase. Some of them are enhanced by enzymes.
Anti-C, anti-c, anti-E, and anti-e - ANSWER Rh antibodies enhanced by enzymes
IgM antibodies - ANSWER Anti-I
Anti-H
Anti-M
Anti-N
Anti-P1
Anti-Le a
Anti-Le b
IgG antibodies - ANSWER Anti-D
Anti-C
Anti-c
Anti-E
Anti-e
Anti-K
Anti-k
Anti-Fya
1
,Anti-Fyb
Anti-Jka
Anti-Jkb
and sometimes anti-M
Lewis - ANSWER Plasma antigens that adsorb onto red blood cells but are not found on
cord cells.
IgM antibody that can be hemolytic but doesn't cause HDFN
B positive - ANSWER
B negative - ANSWER
O negative - ANSWER
O positive - ANSWER
AB positive - ANSWER
AB negative - ANSWER
A negative - ANSWER
Acquired B phenomenon - ANSWER Phenomenon that occurs when blood is exposed to
gram negative bacteria, causing the bacterial deacetylase to convert N-acetylgalactosamine
(Blood group A specific sugar) to ɑ-galactosamine. ɑ-Galactosamine is close enough to galac-
tose that cells agglutinate and cause a discrepant blood type result.
2
,Wash cells - ANSWER The resolution of rouleaux causing a discrepant blood typing result
in the forward type.
Check transfusion history - ANSWER Resolution of a mixed field result in blood typing
Check patient history - ANSWER Resolution when forward type results are discrepant,
presenting a possible acquired B phenomenon.
Saline replacement - ANSWER Resolution when reverse typing presents rouleaux due to
increased serum protein.
Ex: Waldenstrom's or Multiple Myeloma
Cold screen/panel - ANSWER Resolution when reverse typing presents a possible cold an-
tibody
Check patient age - ANSWER Resolution to missing antibodies in reverse typing.
Mini cold panel - ANSWER Used to enhance serum anti-A and anti-B reactions when they
are expected but are not demonstrable using room temp readings, and identify "cold" anti-
bodies reacting with other antigens on A1 and B reagent red cells.
saline replacement - ANSWER Procedure that differentiates rouleaux (aggregation) from
agglutination by replacing the serum in the test mixture with saline. When the cells are
mixed with saline the cells dissociate.
D - ANSWER What is this antigen?
3
, d - ANSWER What is this antigen?
D antigen - ANSWER The most immunogenic of all blood group antigens.
Found in the immediate spin or AHG phase of blood typing.
Weak D - ANSWER Antigen negative at immediate spin but positive in the AHG phase of
testing, validated by a negative control.
aseptic technique - ANSWER scrub site of collection for a minimum of 30 secs
providine-iodine - ANSWER solution used to sterilize puncture site for blood collection of
allogeneic blood donation.
</= 10.5mL of WB/ kg of body weight - ANSWER Maximum collection for allogeneic
blood donation
donor interval - ANSWER 8 weeks after whole blood donation
48hrs - ANSWER platelet inhibiting medications can't be taken _____ prior to allogeneic
blood/platelet donation.
>/= 16yrs - ANSWER minimum age requirement for allogeneic blood donation
37.5°C/99.5°F or colder - ANSWER minimum temperature requirements for allogeneic
blood donation
>/= 12.5g/dL - ANSWER Minimum hemoglobin requirements for allogeneic blood dona-
tion
4
with hemolytic anemia.
Rh antibodies - ANSWER Clinically significant antibodies (IgG) that may agglutinate at 37C
but will agglutinate in the AHG phase. Some of them are enhanced by enzymes.
Anti-C, anti-c, anti-E, and anti-e - ANSWER Rh antibodies enhanced by enzymes
IgM antibodies - ANSWER Anti-I
Anti-H
Anti-M
Anti-N
Anti-P1
Anti-Le a
Anti-Le b
IgG antibodies - ANSWER Anti-D
Anti-C
Anti-c
Anti-E
Anti-e
Anti-K
Anti-k
Anti-Fya
1
,Anti-Fyb
Anti-Jka
Anti-Jkb
and sometimes anti-M
Lewis - ANSWER Plasma antigens that adsorb onto red blood cells but are not found on
cord cells.
IgM antibody that can be hemolytic but doesn't cause HDFN
B positive - ANSWER
B negative - ANSWER
O negative - ANSWER
O positive - ANSWER
AB positive - ANSWER
AB negative - ANSWER
A negative - ANSWER
Acquired B phenomenon - ANSWER Phenomenon that occurs when blood is exposed to
gram negative bacteria, causing the bacterial deacetylase to convert N-acetylgalactosamine
(Blood group A specific sugar) to ɑ-galactosamine. ɑ-Galactosamine is close enough to galac-
tose that cells agglutinate and cause a discrepant blood type result.
2
,Wash cells - ANSWER The resolution of rouleaux causing a discrepant blood typing result
in the forward type.
Check transfusion history - ANSWER Resolution of a mixed field result in blood typing
Check patient history - ANSWER Resolution when forward type results are discrepant,
presenting a possible acquired B phenomenon.
Saline replacement - ANSWER Resolution when reverse typing presents rouleaux due to
increased serum protein.
Ex: Waldenstrom's or Multiple Myeloma
Cold screen/panel - ANSWER Resolution when reverse typing presents a possible cold an-
tibody
Check patient age - ANSWER Resolution to missing antibodies in reverse typing.
Mini cold panel - ANSWER Used to enhance serum anti-A and anti-B reactions when they
are expected but are not demonstrable using room temp readings, and identify "cold" anti-
bodies reacting with other antigens on A1 and B reagent red cells.
saline replacement - ANSWER Procedure that differentiates rouleaux (aggregation) from
agglutination by replacing the serum in the test mixture with saline. When the cells are
mixed with saline the cells dissociate.
D - ANSWER What is this antigen?
3
, d - ANSWER What is this antigen?
D antigen - ANSWER The most immunogenic of all blood group antigens.
Found in the immediate spin or AHG phase of blood typing.
Weak D - ANSWER Antigen negative at immediate spin but positive in the AHG phase of
testing, validated by a negative control.
aseptic technique - ANSWER scrub site of collection for a minimum of 30 secs
providine-iodine - ANSWER solution used to sterilize puncture site for blood collection of
allogeneic blood donation.
</= 10.5mL of WB/ kg of body weight - ANSWER Maximum collection for allogeneic
blood donation
donor interval - ANSWER 8 weeks after whole blood donation
48hrs - ANSWER platelet inhibiting medications can't be taken _____ prior to allogeneic
blood/platelet donation.
>/= 16yrs - ANSWER minimum age requirement for allogeneic blood donation
37.5°C/99.5°F or colder - ANSWER minimum temperature requirements for allogeneic
blood donation
>/= 12.5g/dL - ANSWER Minimum hemoglobin requirements for allogeneic blood dona-
tion
4
