AMT MLS EXAM NEWEST 2025/ 2026 ACTUAL EXAM TEST BANK|
COMPLETE 200 REAL EXAM QUESTIONS AND CORRECT DETAILED
ANSWERS (VERIFIED ANSWERS) ALREADY GRADED A+| AMT MEDICAL
LABORATORY SCIENTIST CERTIFICATION EXAM PREP (BRAND NEW!!)
Which immunoassay methodology is the emission of light by molecules in an excited state with a
limited amount of heat as the result of a chemical reaction?
Question
a.Chemiluminescence
b.Immunochromatography
c.Photometry
d.Spectrophotometry - correct answer -a. Chemiluminescence
Chemiluminescence is the emission of light by molecules in an excited state with a limited amount of
heat (luminescence) as the result of a chemical reaction. Chemiluminescent assays are ultrasensitive and
are widely used in automated immunoassays and DNA probe assay systems. Chemiluminescence has
excellent sensitivity and dynamic range. Chemiluminescent labels can be attached to an antigen or an
antibody. Most chemiluminescent reagents and conjugates are stable and relatively nontoxic.
What characteristics/functions do calibrators have?
Question
a.Contains a known amount of analyte being tested
b.Monitors the quality of reagents
c.Monitors the quality of the sample
d.Prevents equipment failure - correct answer -a. Contains a known amount of analyte being tested
Calibrators are used to establish a correlation between the measurement response and the
concentration or amount of the substance that is being measured by the test procedure.
Absorbance can be calculated easily from percent transmission using which calculation?
a.2 - log10 %T
b.2 + log10 %T
c.log10 %T - 2
d.log10 %T + 2 - correct answer -a. 2 - log10 %T
,Absorbance can be calculated easily from percent transmission using 2 - log10 %T. If all the light passes
through a solution without any absorption, absorbance is zero and the %T is 100%. If all the light is
absorbed, %T is zero, and absorption is infinite.
What is a substance or constituent in which the laboratory conducts testing?
a.A solution
b.An additive
c.An analyte
d.An anticoagulant - correct answer -c. An analyte
In the testing process, analytes or constituents are measured.
What does a common application of the polarized light microscope in the clinical laboratory do?
Determine FANAs with fluorescence
b.Determine neutrophilic from lymphocytic leukemia
c.Examine histochemical reactions
d.Study crystals in urine and in cells - correct answer -d. study crystals in urine and cells
What is the chelation of calcium in the EDTA tube?
a.Maintains a constant temperature for components
b.Prevents coagulation by inhibiting coagulation
c.Prevents the separation of blood components
d.Stops the lysing of the red blood cells - correct answer -b. Prevents coagulation by inhibiting
coagulation
CLSI recommends the salts of the chelating (calcium binding) agent EDTA as the anticoagulant of choice
for blood cell counting and sizing.
How must a laboratory operate to handle TB sputum and TM materials?
a.Biosafety level of 2+ or 3
b.Biosafety level 4
,c.Location where air exchange is continuous
d.Location where fume hood exhausts are monitored daily - correct answer -a. Biosafety level of 2+ or
3
BSL-3 builds upon the requirements of BSL-2. Organisms that can cause serious or potentially lethal
disease through respiratory transmission are typically worked with in a BSL-3 laboratory. Example:
Mycobacterium tuberculosis.
What are Class A fire extinguishers used for?
a.Electric equipment
b.Flammable liquids
c.Ordinary combustibles
d.Powdered metal (combustible) material - correct answer -Ordinary combustibles.
Class A fire extinguishers are classified as ordinary combustible. Additionally, Class A fire extinguishers
have a numerical rating based on UL-conducted tests and designed to determine the extinguishing
potential for each size and type of extinguisher
Which part of the microscope directs and focuses the beam of light from the bulb onto the material
under examination?
a.Aperture
b.C- mount
c.Condenser
d.Diaphragm - correct answer -Condenser
The condenser is the substage lens of the microscope that focuses light on the specimen slide
How many milliliters can a 3% solution make if 6 grams of solute are available?
a.100 mL
b.200 mL
c.300 mL
d.400 mL - correct answer -Correct Answer: 200 mL
3% of solution means 3 g of solute in 100 mL of solution.
, 1 g of solute contains = (100/3) mL
So, in 6 g of solute = (100/3) x 6 =200 mL.
200 mL of 3% solution is needed for 6 g of solute.
When seven points in a row in an upward or downward direction are seen on a quality control graph it
is called a
a.deviation.
b.shift.
c.trend.
d.variance. - correct answer -trend.
A trend is seven points in a row in an upward direction or seven points in a row in a downward direction.
With a trend, it doesn't matter if the centerline is crossed
What category of bioterrorism agents include pathogens that are moderately easy to disseminate,
result in moderate and low morbidity rates, and require specific enhancements of the CDC's
diagnostic capacity and enhanced disease surveillance?
a.Category A
b.Category B
c.Category C
d.Category D - correct answer -Category B
Bioterrorism agents are a concern to laboratories. These agents are divided into categories A, B and C;
and Category B agents have the second highest priority. Category B agents have moderate morbidity
(suffering from the disease) with low mortality (death) rates. Ricin toxin is an example of Category B
agent.
What category of bioterrorism agents have the highest priority and pose a risk to national security
because they can be easily disseminated or transmitted from person to person, result in high
mortality rates and have the potential for major public health impact?
a.Category A
b.Category B
c.Category C
COMPLETE 200 REAL EXAM QUESTIONS AND CORRECT DETAILED
ANSWERS (VERIFIED ANSWERS) ALREADY GRADED A+| AMT MEDICAL
LABORATORY SCIENTIST CERTIFICATION EXAM PREP (BRAND NEW!!)
Which immunoassay methodology is the emission of light by molecules in an excited state with a
limited amount of heat as the result of a chemical reaction?
Question
a.Chemiluminescence
b.Immunochromatography
c.Photometry
d.Spectrophotometry - correct answer -a. Chemiluminescence
Chemiluminescence is the emission of light by molecules in an excited state with a limited amount of
heat (luminescence) as the result of a chemical reaction. Chemiluminescent assays are ultrasensitive and
are widely used in automated immunoassays and DNA probe assay systems. Chemiluminescence has
excellent sensitivity and dynamic range. Chemiluminescent labels can be attached to an antigen or an
antibody. Most chemiluminescent reagents and conjugates are stable and relatively nontoxic.
What characteristics/functions do calibrators have?
Question
a.Contains a known amount of analyte being tested
b.Monitors the quality of reagents
c.Monitors the quality of the sample
d.Prevents equipment failure - correct answer -a. Contains a known amount of analyte being tested
Calibrators are used to establish a correlation between the measurement response and the
concentration or amount of the substance that is being measured by the test procedure.
Absorbance can be calculated easily from percent transmission using which calculation?
a.2 - log10 %T
b.2 + log10 %T
c.log10 %T - 2
d.log10 %T + 2 - correct answer -a. 2 - log10 %T
,Absorbance can be calculated easily from percent transmission using 2 - log10 %T. If all the light passes
through a solution without any absorption, absorbance is zero and the %T is 100%. If all the light is
absorbed, %T is zero, and absorption is infinite.
What is a substance or constituent in which the laboratory conducts testing?
a.A solution
b.An additive
c.An analyte
d.An anticoagulant - correct answer -c. An analyte
In the testing process, analytes or constituents are measured.
What does a common application of the polarized light microscope in the clinical laboratory do?
Determine FANAs with fluorescence
b.Determine neutrophilic from lymphocytic leukemia
c.Examine histochemical reactions
d.Study crystals in urine and in cells - correct answer -d. study crystals in urine and cells
What is the chelation of calcium in the EDTA tube?
a.Maintains a constant temperature for components
b.Prevents coagulation by inhibiting coagulation
c.Prevents the separation of blood components
d.Stops the lysing of the red blood cells - correct answer -b. Prevents coagulation by inhibiting
coagulation
CLSI recommends the salts of the chelating (calcium binding) agent EDTA as the anticoagulant of choice
for blood cell counting and sizing.
How must a laboratory operate to handle TB sputum and TM materials?
a.Biosafety level of 2+ or 3
b.Biosafety level 4
,c.Location where air exchange is continuous
d.Location where fume hood exhausts are monitored daily - correct answer -a. Biosafety level of 2+ or
3
BSL-3 builds upon the requirements of BSL-2. Organisms that can cause serious or potentially lethal
disease through respiratory transmission are typically worked with in a BSL-3 laboratory. Example:
Mycobacterium tuberculosis.
What are Class A fire extinguishers used for?
a.Electric equipment
b.Flammable liquids
c.Ordinary combustibles
d.Powdered metal (combustible) material - correct answer -Ordinary combustibles.
Class A fire extinguishers are classified as ordinary combustible. Additionally, Class A fire extinguishers
have a numerical rating based on UL-conducted tests and designed to determine the extinguishing
potential for each size and type of extinguisher
Which part of the microscope directs and focuses the beam of light from the bulb onto the material
under examination?
a.Aperture
b.C- mount
c.Condenser
d.Diaphragm - correct answer -Condenser
The condenser is the substage lens of the microscope that focuses light on the specimen slide
How many milliliters can a 3% solution make if 6 grams of solute are available?
a.100 mL
b.200 mL
c.300 mL
d.400 mL - correct answer -Correct Answer: 200 mL
3% of solution means 3 g of solute in 100 mL of solution.
, 1 g of solute contains = (100/3) mL
So, in 6 g of solute = (100/3) x 6 =200 mL.
200 mL of 3% solution is needed for 6 g of solute.
When seven points in a row in an upward or downward direction are seen on a quality control graph it
is called a
a.deviation.
b.shift.
c.trend.
d.variance. - correct answer -trend.
A trend is seven points in a row in an upward direction or seven points in a row in a downward direction.
With a trend, it doesn't matter if the centerline is crossed
What category of bioterrorism agents include pathogens that are moderately easy to disseminate,
result in moderate and low morbidity rates, and require specific enhancements of the CDC's
diagnostic capacity and enhanced disease surveillance?
a.Category A
b.Category B
c.Category C
d.Category D - correct answer -Category B
Bioterrorism agents are a concern to laboratories. These agents are divided into categories A, B and C;
and Category B agents have the second highest priority. Category B agents have moderate morbidity
(suffering from the disease) with low mortality (death) rates. Ricin toxin is an example of Category B
agent.
What category of bioterrorism agents have the highest priority and pose a risk to national security
because they can be easily disseminated or transmitted from person to person, result in high
mortality rates and have the potential for major public health impact?
a.Category A
b.Category B
c.Category C
