Optimization and Validation of an HPLC Method for Detecting Plasma Vitamin C

Optimization and Validation of an HPLC Method for Detecting Plasma Vitamin C LIST OF TABLES LIST OF FIGURES CHAPTER 1 INTRODUCTION 2 REVIEW OF LITERATURE Theme A: Vitamin C Theme B: Methods of Detection and Quantification TABLE OF CONTENTS Page .iii .iv 1 6 6 8 Theme C: High Performance Liquid Chromatography. 11 3 METHODS 16 4 RESULTS 20 5 DISCUSSION 6 CONCLUSION REFERENCES APPENDIX A IRB APPROVAL B SOLUTION RECIPES 29 31 32 8 10 i Discuss this document with others on S Studydrive LIST OF TABLES Table Page 1. Parameters and Descriptors of UV-HPLC 17 2. Sample Volume and Dilutions. 18 3. Standard Runs Inter and Intra Day % CV 20 4. Inter and Intra Day Percent Recoveries for Standards and Samples 21 5. Standards Inter and Intra Day %CV. 22 6. Standard Run Day 1 Concentrations and Percent Recoveries 22 7. Sample Run Day 1 with 40 µL of Sample 24 8. Sample Run Day 2 Run 1 26 9. Sample Run day 2 run 2 26 10. Sample Run Day 3 with and without Spiked AA 28 iii Quiz yourself with flashcards on Studydrive LIST OF FIGURES Figure Page 1. Day 1 Calibration Curve 2. Day 1 Samples Peaks 3. Day 2 Standards Calibration Curve Run 1. 4. Day 2 Standards Peaks Run 1 5. Day 2 Calibration Curve Run 2 6. Day 2 Standards Peaks Run 2 7. Day 3 Calibration Curve 8. Day 3 Standard Peaks