D103 midterm Questions With Complete Solutions
As a result of a mutation that disrupts the nuclear localization
signal of a protein, the protein will now be. Correct Answers be
remained in the cytoplasm
At MTOCs, microtubule nucleation is facilitated by Correct
Answers γ-tubulinring complex
Cell growth is commonly regulated by signaling receptors,
whose levels at the cell surface are tightly controlled. Receptor
downregulation occurs by clathrin-mediated endocytosis and
degradation in the lysosome. What would be the consequences
of a mutation in the AP2 adaptor, which is necessary for
clathrin-mediated endocytosis? Correct Answers Receptor
remains at the cell surface leading to enhanced cell growth
During skeleton muscle contraction, tropomodulin consistently
binds to F-actin (-) end. What is the consequence of this
tropomodulin binding? Correct Answers the F-actin (-) end is
stabilized
From the D103 class, you know the role of myosins actin
regulation. There are many members in myosin family. Which
of the following domains is shared by all myosins? Correct
Answers ATP-binding domain
How would inhibition of Rac GEF protein affect cell migration?
Correct Answers Cells would not migrate
, How would you determine if a protein is mono-or poly-
ubiquitinated? Correct Answers Prepare protein lysate and
determine size of ubiquitinated protein by western blot analysis
How would you determine if protein X is required for cell
migration? Correct Answers Disrupt protein X and measure
cell migration
How would you test whether actin cytoskeleton promotes
mitochondria fission process? Correct Answers Prevent F-actin
assembly and examine mitochondria dynamics by monitoring a
GFP tagged ATP Synthase.
Imagine a small synthetic vesicle made from pure
phospholipids(formed as lipid bilayer). Its lumen contains 1 mM
Glucose and 1 mM sodium chloride. If the vesicle is placed in
pure water, which of the following happens fastest? Correct
Answers H2O diffused in
In an in vitro polymerization reaction of F-actin from its G-
actinsubunits, there is a lag phase. What happens during this lag
phase? Correct Answers F-actin is nucleated.
In contrast to transporters, channel proteins in cellular
membrane Correct Answers Can only mediate passive
transport
One of the globular head domains of a dynein molecule is
mutated so that it can no longer bind ATP.How does this
mutation affect dynein movement along a microtubule? Correct
As a result of a mutation that disrupts the nuclear localization
signal of a protein, the protein will now be. Correct Answers be
remained in the cytoplasm
At MTOCs, microtubule nucleation is facilitated by Correct
Answers γ-tubulinring complex
Cell growth is commonly regulated by signaling receptors,
whose levels at the cell surface are tightly controlled. Receptor
downregulation occurs by clathrin-mediated endocytosis and
degradation in the lysosome. What would be the consequences
of a mutation in the AP2 adaptor, which is necessary for
clathrin-mediated endocytosis? Correct Answers Receptor
remains at the cell surface leading to enhanced cell growth
During skeleton muscle contraction, tropomodulin consistently
binds to F-actin (-) end. What is the consequence of this
tropomodulin binding? Correct Answers the F-actin (-) end is
stabilized
From the D103 class, you know the role of myosins actin
regulation. There are many members in myosin family. Which
of the following domains is shared by all myosins? Correct
Answers ATP-binding domain
How would inhibition of Rac GEF protein affect cell migration?
Correct Answers Cells would not migrate
, How would you determine if a protein is mono-or poly-
ubiquitinated? Correct Answers Prepare protein lysate and
determine size of ubiquitinated protein by western blot analysis
How would you determine if protein X is required for cell
migration? Correct Answers Disrupt protein X and measure
cell migration
How would you test whether actin cytoskeleton promotes
mitochondria fission process? Correct Answers Prevent F-actin
assembly and examine mitochondria dynamics by monitoring a
GFP tagged ATP Synthase.
Imagine a small synthetic vesicle made from pure
phospholipids(formed as lipid bilayer). Its lumen contains 1 mM
Glucose and 1 mM sodium chloride. If the vesicle is placed in
pure water, which of the following happens fastest? Correct
Answers H2O diffused in
In an in vitro polymerization reaction of F-actin from its G-
actinsubunits, there is a lag phase. What happens during this lag
phase? Correct Answers F-actin is nucleated.
In contrast to transporters, channel proteins in cellular
membrane Correct Answers Can only mediate passive
transport
One of the globular head domains of a dynein molecule is
mutated so that it can no longer bind ATP.How does this
mutation affect dynein movement along a microtubule? Correct
