NHCO Phlebotomy Exam NEWEST
2025/2026 Actual Exam – 250 Real
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[SECTION 1: Phlebotomy Fundamentals & Safety — Questions 1-35]
Q1: According to OSHA's Bloodborne Pathogens Standard, which is the primary engineering
control designed to protect the phlebotomist from needlestick injuries?
A. Wearing double gloves
B. Using a laboratory coat
C. Safety-engineered sharps devices (e.g., retractable needles) [CORRECT]
D. Biohazard disposal containers
Correct Answer: C
Rationale: Engineering controls are devices that isolate or remove the hazard, such as safety
needles with shielding or retraction mechanisms. While PPE like gloves (A) and lab coats (B) are
necessary work practice controls, the primary engineering control aimed at preventing the initial
injury is the safety device itself. Biohazard containers (D) are for disposal, not prevention of the
stick during the procedure.
Q2: What is the first action a phlebotomist must take immediately after a needlestick injury?
A. Fill out an incident report.
B. Immediately administer the Hepatitis B vaccine.
C. Wash the area with soap and water. [CORRECT]
D. Apply a strong antiseptic like bleach.
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Correct Answer: C
Rationale: Immediate cleansing of the wound with soap and water is the critical first step to
reduce viral load. Filling out the report (A) is essential documentation but happens immediately
after first aid. The Hepatitis B vaccine (B) or post-exposure prophylaxis (PEP) is prescribed by a
medical professional after the exposure evaluation. Bleach (D) is a chemical irritant and should
never be used on skin.
Q3: Which of the following is considered a violation of patient confidentiality under HIPAA?
A. Discussing a patient's blood draw difficulty with a supervisor in a private office.
B. Leaving a patient's chart face down on a counter in a designated area.
C. Verifying a patient's identity by asking their name and date of birth in the waiting room.
[CORRECT]
D. Releasing lab results to the ordering physician.
Correct Answer: C
Rationale: While confirming identity is necessary, doing so in a public waiting area where others
can hear personal health information (PHI) is a privacy violation. Discussions should occur in a
private setting. Charts (B) must be protected, but face down in a designated secure area is
generally acceptable compared to open display. Releasing results to the physician (D) is standard
practice.
Q4: How long should a phlebotomist perform hand hygiene using soap and water?
A. 5-10 seconds
B. Until the hands feel clean
C. 15-20 seconds [CORRECT]
D. 1 minute
Correct Answer: C
Rationale: CDC and CLSI guidelines recommend scrubbing hands with soap and water for at
least 15-20 seconds to effectively remove soil and transient microorganisms. 5-10 seconds (A) is
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insufficient. Hand sanitizer is an alternative if hands are not visibly soiled, but soap and water
requires the full 20-second duration.
Q5: Which Personal Protective Equipment (PPE) is required for routine venipuncture?
A. Gown
B. Face shield
C. Gloves [CORRECT]
D. Mask
Correct Answer: C
Rationale: Gloves are the standard PPE required for all venipuncture procedures to prevent
contact with blood and body fluids. Gowns (A), face shields (B), and masks (D) are typically
reserved for procedures with a high risk of splash or spray, such as arterial blood gas collection
or trauma phlebotomy, rather than standard venipuncture.
Q6: When should a phlebotomist change gloves during a routine blood draw?
A. Only if the gloves are visibly torn.
B. After every patient encounter.
C. After every patient encounter. [CORRECT]
D. Only if the patient has a known infectious disease.
Correct Answer: B
Rationale: Standard Precautions dictate that gloves must be changed and hands washed between
every single patient encounter to prevent cross-contamination. Waiting until gloves are torn (A)
or only for known diseases (D) violates safety protocols because infection status is not always
known.
Q7: The purpose of the "tourniquet" is to:
A. Sterilize the venipuncture site.
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B. Prevent the vein from rolling.
C. Enlarge the vein by restricting venous return. [CORRECT]
D. Numb the puncture site.
Correct Answer: C
Rationale: The tourniquet applies pressure to the venous return, causing blood to pool in the
veins, which makes them larger, more prominent, and easier to puncture. It does not sterilize (A)
or numb (D) the site. Stabilizing the vein (B) is done by the phlebotomist's hand, not the
tourniquet.
Q8: What is the most critical step in the collection process to ensure patient safety and specimen
integrity?
A. Selecting the correct needle gauge.
B. Applying the bandage after the draw.
C. Positive patient identification. [CORRECT]
D. Mixing the tubes gently.
Correct Answer: C
Rationale: Positive patient identification (using at least two unique identifiers) is the most critical
error-prevention strategy to ensure the specimen belongs to the correct patient (Wrong Patient in
Wrong Bed). Errors here can lead to misdiagnosis and inappropriate treatment. While needle
gauge (A) and mixing (D) are important, identifying the patient is the foundational safety step.
Q9: Which of the following statements regarding "Standard Precautions" is correct?
A. Only patients with visible symptoms are considered infectious.
B. All blood and body fluids are treated as potentially infectious. [CORRECT]
C. Precautions are only necessary in the laboratory.
D. Eye protection is optional during venipuncture.