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PNB 2265 Practical 2 Study Guide | 400+ Questions and Answers on Respiratory Physiology, Spirometry, Urinalysis, Renal Anatomy & Histology

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This comprehensive PNB 2265 Practical 2 Study Guide contains more than 400 expertly verified questions and answers covering respiratory physiology, spirometry, pulmonary function testing, urinalysis, renal physiology, glucose absorption, histology identification, and laboratory-based anatomy and physiology concepts. The document provides detailed explanations of tidal volume, inspiratory reserve volume, expiratory reserve volume, residual volume, lung capacities, FEV1/FVC ratios, respiratory acidosis, respiratory alkalosis, pulmonary ventilation, restrictive and obstructive lung diseases, emphysema, COPD, tuberculosis, and respiratory mechanics during inhalation and exhalation. It also reviews airflow pathways, lung anatomy, laryngeal structures, bronchi, alveoli, respiratory bronchioles, tracheal histology, and pulmonary tissue identification. The material further explores advanced renal physiology and urinalysis concepts including urine pH, specific gravity, glucose and protein testing, ketone analysis, refractometry, glycosuria, proteinuria, diabetes mellitus indicators, nephritis, diabetic ketoacidosis, and urine chloride concentration testing. Additional laboratory concepts include glucose standard curve preparation, spectrophotometry, DNS reagent analysis, secondary active transport of glucose, fructose absorption, Na+/K+ ATPase function, GLUT transporters, SGLT cotransporters, effects of DNP on ATP production, and physiological changes observed during experimental glucose transport conditions. The study guide also includes practical explanations of fluid balance responses to water versus electrolyte drinks and the physiological effects on urine concentration and osmolality. The document additionally reviews major anatomy and histology identification topics including kidney structures, Bowman's capsule, glomerulus, proximal and distal convoluted tubules, stomach anatomy, salivary glands, pancreas, liver, small intestine, trachea, esophagus, and respiratory tissue samples. Detailed labeling questions and tissue identification exercises are integrated throughout the guide to support laboratory practical preparation and anatomy competency assessments. The organized question-and-answer format simplifies difficult respiratory, renal, and gastrointestinal physiology concepts while strengthening understanding of laboratory methods and histological analysis commonly tested in physiology and health science programs. This study resource is highly beneficial for neuroscience students, physiology students, nursing students, biology majors, respiratory therapy students, pre-med students, allied health learners, healthcare science students, physician assistant students, and medical laboratory science students preparing for practical exams, laboratory assessments, cumulative finals, anatomy and physiology coursework, and pulmonary or renal physiology evaluations. The structured review format promotes active recall learning, improves retention of laboratory-based physiological concepts, and strengthens understanding of complex organ system functions and diagnostic testing methods. The content aligns with foundational respiratory physiology, renal physiology, histology, and laboratory science concepts commonly taught in undergraduate health science programs and reflects material referenced in academic textbooks including Guyton and Hall Textbook of Medical Physiology, Human Physiology by Silverthorn, Vander’s Renal Physiology, Respiratory Physiology: The Essentials by John B. West, and Junqueira’s Basic Histology. Topics related to spirometry, pulmonary disease, urinalysis, glucose transport, nephron anatomy, respiratory mechanics, tissue histology, and laboratory physiology are consistent with evidence-based educational standards used across neuroscience, biology, physiology, nursing, respiratory therapy, and healthcare science programs at colleges and universities. Keywords PNB 2265, respiratory physiology, spirometry, pulmonary function testing, respiratory system study guide, lung volumes, tidal volume, inspiratory reserve volume, expiratory reserve volume, residual volume, vital capacity, total lung capacity, inspiratory capacity, functional residual capacity, FEV1, FVC, respiratory acidosis, respiratory alkalosis, COPD, emphysema, restrictive lung disease, pulmonary fibrosis, tuberculosis, pulmonary compliance, respiratory mechanics, inhalation physiology, exhalation physiology, alveoli, bronchioles, trachea anatomy, lung histology, respiratory bronchioles, pulmonary anatomy, pulmonary physiology, spirometer, pulmonary exam review, urinalysis, urine specific gravity, urine pH, proteinuria, glycosuria, ketones in urine, diabetes mellitus, nephritis, diabetic ketoacidosis, refractometer, renal physiology, glucose transport, secondary active transport, Na K ATPase, GLUT2, GLUT5, SGLT transporter, spectrophotometry, DNS reagent, glucose standard curve, DNP physiology, glucose absorption, intestinal transport, renal anatomy, Bowmans capsule, glomerulus, proximal convoluted tubule, distal convoluted tubule, kidney histology, nephron anatomy, anatomy practical review, histology identification, gastrointestinal histology, liver histology, pancreas histology, trachea histology, stomach anatomy, esophagus histology, larynx anatomy, healthcare science review, nursing physiology, respiratory therapy review, physiology practical exam, anatomy and physiology questions, laboratory physiology, pulmonary disease review, renal lab review, tissue identification, advanced physiology concepts, medical laboratory science, biology study guide, pre med physiology, human physiology review, physiology questions and answers

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PNB 2265: Practical 2

what is spirometry used for - ANSWER ✔✔used to measure the

amount of air that can be inhaled and exhaled from the lungs

why is the subject's nose clipped when using a spirometer -

ANSWER ✔✔ensures that the subject's respiratory tract and the

spirometer form a closed system


what is tidal volume - ANSWER ✔✔amount of air inhaled and

exhaled during normal breathing / quiet breathing


what is inspiratory reserve volume - ANSWER ✔✔amount of air that

can be forcefully inhaled after a normal tidal volume inhalation

,what is expiratory reserve volume - ANSWER ✔✔amount of air that

can be forcefully exhaled after a normal tidal volume exhalation


what is residual volume - ANSWER ✔✔amount of air remaining in the

lungs after a forced expiration


what is lung capacity - ANSWER ✔✔the sum of two or more lung

volumes


what is vital capacity - ANSWER ✔✔- the sum of the inspiratory

reserve volume, expiratory reserve volume, and tidal volume

- represents the maximum amount of air that can be voluntarily moved

into or out of the respiratory system with each breath


what is total lung capacity - ANSWER ✔✔vital capacity plus the

residual volume


what is inspiratory capacity - ANSWER ✔✔tidal volume plus the

inspiratory reserve volume


what is functional residual capacity - ANSWER ✔✔the expiratory

reserve volume plus the residual volume

what is ratio is used as an indicator of pulmonary function and why -

ANSWER ✔✔FEV1 / FVC = 80 (normal)

, - FEV1 = forced expiratory volume of 1 second

- FVC = total amount of air a person can exhale

- on average, a person can exhale 80% of their forced vital capacity in a

single second


when does respiratory acidosis occur - ANSWER ✔✔- when the

respiratory system is unable to eliminate enough CO2 through

exhalation causing pCO2 inside the blood to increase --> increased

carbonic acid formation

- due to hypoventilation


when does respiratory alkalosis occur - ANSWER ✔✔- when CO2 is

removed from the body at a rate faster than normal and the pCO2 inside

the blood drops --> decreased carbonic acid

- due to increased ventilation / hyperventilation

- also can occur due to hypoxia


what is the normal pH of the body - ANSWER ✔✔7.35


respiratory acidosis vs metabolic acidosis - ANSWER ✔✔respiratory

acidosis

- when respiratory function is impaired and causes retention of CO2



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