UNE MEDICAL BIOCHEMISTRY UNIT 1
PRACTICE SET 2026 EXAM QUESTIONS
AND CORRECT SOLUTIONS A+
◉ An α-helical arrangement of amino acids is considered to be part
of what level of protein structure?
Answer: Secondary structure of the protein
◉ The malate-aspartate shuttle is used to move reducing equivalents
of cytosolic NADH into the mitochondria where it can be oxidized.
Which of the following compounds is used to move the reducing
equivalents from cytosolic NADH into the mitochondria?
a) aspartate
b) malate
c) glutamate
d) oxaloacetate
Answer: Malate
◉ What is the [OH] concentration in a 0.001 M = 10-3 M solution of
HCl.
(10-14 = [H+] [OH-])
,Answer: 10^-11 M
◉ Which of the following would be consistent with an exergonic
reaction?
Answer: ΔG0 would be negative
◉ Hemoglobin has the ability to display cooperative binding while
myoglobin does not display this binding kinetic pattern. Which of
the following differences between the two proteins accounts for this
difference in binding kinetics?
Answer: The presence of quaternary structure in hemoglobin
◉ Which of the following would be consistent with an exergonic
reaction?
a) ΔG would be negative
b) ΔS would be negative
c) ΔS would be positive
d) ΔG would be positive
Answer: ΔG would be negative
◉ Water is a key component of biological systems. Which of the
following statements is false with regards to the chemical properties
of water?
,Answer: Water has a very large dissociation constant
◉ Complex II (Succinate Q-reductase) is not considered to be part of
the process of oxidative phosphorylation because it is unable to do
which of the following activities?
Answer: Pump protons from the mitochondrial matrix to the
intermembrane space
◉ As electrons flow through complexes in the inner mitochondrial
membrane, protons are pumped from the matrix to the
intermembrane space. Which of the following statements is most
reflective of the pH in the intermembrane space?
Answer: The pH is higher in the matrix when compared to the
intermembrane space.
◉ Dinitrophenol is an uncoupler of the electron transport chain. The
addition of dinitrophenol will have which of the following impacts?
a) decrease the pH of the intermembrane space
b) increase oxygen consumption
c) increase ATP production
d) decrease oxidation of NADH
Answer: increase oxygen consumption
, ◉ ATP is a high energy molecule that is often hydrolyzed during
reactions. The energy within ATP is stored primarily in which of the
following components of the molecule?
a) in the adenine base
b) in the high energy phosphate bonds
c) in the D-ribose group
Answer: in the high energy phosphate bonds
◉ Plasma membrane
Answer: lipid bilayer serves as a selective barrier
◉ An example of enzyme activation by cleavage is best illustrated by
which of the following?
Answer: Activation of chymotrypsinogen to chymotrypsin
◉ ATP is a high energy molecule that is often hydrolyzed during
reactions. The energy within ATP is stored primarily in which of the
following components of the molecule?
Answer: In the high energy phosphate bonds
◉ integral membrane proteins
Answer: facilitate the transport of compounds by active transport,
facilitated diffusion, or by forming pores or gated channels
PRACTICE SET 2026 EXAM QUESTIONS
AND CORRECT SOLUTIONS A+
◉ An α-helical arrangement of amino acids is considered to be part
of what level of protein structure?
Answer: Secondary structure of the protein
◉ The malate-aspartate shuttle is used to move reducing equivalents
of cytosolic NADH into the mitochondria where it can be oxidized.
Which of the following compounds is used to move the reducing
equivalents from cytosolic NADH into the mitochondria?
a) aspartate
b) malate
c) glutamate
d) oxaloacetate
Answer: Malate
◉ What is the [OH] concentration in a 0.001 M = 10-3 M solution of
HCl.
(10-14 = [H+] [OH-])
,Answer: 10^-11 M
◉ Which of the following would be consistent with an exergonic
reaction?
Answer: ΔG0 would be negative
◉ Hemoglobin has the ability to display cooperative binding while
myoglobin does not display this binding kinetic pattern. Which of
the following differences between the two proteins accounts for this
difference in binding kinetics?
Answer: The presence of quaternary structure in hemoglobin
◉ Which of the following would be consistent with an exergonic
reaction?
a) ΔG would be negative
b) ΔS would be negative
c) ΔS would be positive
d) ΔG would be positive
Answer: ΔG would be negative
◉ Water is a key component of biological systems. Which of the
following statements is false with regards to the chemical properties
of water?
,Answer: Water has a very large dissociation constant
◉ Complex II (Succinate Q-reductase) is not considered to be part of
the process of oxidative phosphorylation because it is unable to do
which of the following activities?
Answer: Pump protons from the mitochondrial matrix to the
intermembrane space
◉ As electrons flow through complexes in the inner mitochondrial
membrane, protons are pumped from the matrix to the
intermembrane space. Which of the following statements is most
reflective of the pH in the intermembrane space?
Answer: The pH is higher in the matrix when compared to the
intermembrane space.
◉ Dinitrophenol is an uncoupler of the electron transport chain. The
addition of dinitrophenol will have which of the following impacts?
a) decrease the pH of the intermembrane space
b) increase oxygen consumption
c) increase ATP production
d) decrease oxidation of NADH
Answer: increase oxygen consumption
, ◉ ATP is a high energy molecule that is often hydrolyzed during
reactions. The energy within ATP is stored primarily in which of the
following components of the molecule?
a) in the adenine base
b) in the high energy phosphate bonds
c) in the D-ribose group
Answer: in the high energy phosphate bonds
◉ Plasma membrane
Answer: lipid bilayer serves as a selective barrier
◉ An example of enzyme activation by cleavage is best illustrated by
which of the following?
Answer: Activation of chymotrypsinogen to chymotrypsin
◉ ATP is a high energy molecule that is often hydrolyzed during
reactions. The energy within ATP is stored primarily in which of the
following components of the molecule?
Answer: In the high energy phosphate bonds
◉ integral membrane proteins
Answer: facilitate the transport of compounds by active transport,
facilitated diffusion, or by forming pores or gated channels
