Portage Microbiology Lab Exam 1
1. What three elements are used in an autoclave to sterilize equipment?: 1. Heat
2. Pressure 3. Steam
2. What type of incubator is pictured below?: The picture below is a Fixed Incubator.
3. What is the minimum temperature an autoclave must be set at to achieve sterile
condition?: The minimum temperature for an autoclave to reach sterile conditions is 125°C.
4. If you are working in a lab in which an autoclave is not available, and you are pressed for
time, which would you choose to best sterilize your equipment? Hot steam or hot air?
Explain why you chose your answer.: The best way to sterilize equipment if an autoclave is not available, it
should be hot steam because it will sterilize the equipment in a matter
of minutes while hot air could take hours to reach the same ettect.
5. At what temperature should you refrigerate bacterial samples? Explain why this is beneficial.:
4°C. This temperature slows bacterial growth but does not stop it, thereby preserving the sample.
6. What are the FOUR types of gloves presented in the lab video?: Latex gloves, Nitrile gloves,
Thermal cold gloves, Thermal heat gloves.
7. At what temperature is the fixed incubator set to, as presented in the lab video?: The
fixed incubator should have a temperature of 37°C.
8. What THREE rules were discussed in regards to lab safety that would protect you and
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1. What three elements are used in an autoclave to sterilize equipment?: 1. Heat
2. Pressure 3. Steam
2. What type of incubator is pictured below?: The picture below is a Fixed Incubator.
3. What is the minimum temperature an autoclave must be set at to achieve sterile
condition?: The minimum temperature for an autoclave to reach sterile conditions is 125°C.
4. If you are working in a lab in which an autoclave is not available, and you are pressed for
time, which would you choose to best sterilize your equipment? Hot steam or hot air?
Explain why you chose your answer.: The best way to sterilize equipment if an autoclave is not available, it
should be hot steam because it will sterilize the equipment in a matter
of minutes while hot air could take hours to reach the same ettect.
5. At what temperature should you refrigerate bacterial samples? Explain why this is beneficial.:
4°C. This temperature slows bacterial growth but does not stop it, thereby preserving the sample.
6. What are the FOUR types of gloves presented in the lab video?: Latex gloves, Nitrile gloves,
Thermal cold gloves, Thermal heat gloves.
7. At what temperature is the fixed incubator set to, as presented in the lab video?: The
fixed incubator should have a temperature of 37°C.
8. What THREE rules were discussed in regards to lab safety that would protect you and
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