Answers
Davis-Bacon Act The Davis-Bacon act of 1931 is a United States federal law that establishes the
requirement for paying the local prevailing wages and fringe benefits on public
works projects for laborers and mechanics.
It applies to "contractors and subcontractors performing on federally funded or
assisted contracts in excess of $2,000 for the construction, alteration, or repair
(including painting and decorating) of public buildings or public works".
, Biohazard Level 1 Work involving well-characterized agents not known to consistently cause
disease in immunocompetent adult humans, and present minimal potential hazard
to laboratory personnel and the environment.
Work is typically conducted on open bench tops using standard microbiological
practices. Special containment equipment or facility design is
not required, but may be used as determined by appropriate risk assessment.
Example: Chicken Pox
Biohazard Level 2 Work involving agents that pose moderate hazards to personnel and the
environment.
It differs from BSL-1 in that: 1) laboratory personnel have specific training in
handling pathogenic agents and are supervised by scientists competent in
handling infectious agents and associated procedures; 2) access to the laboratory
is restricted when work is being conducted; and 3) all procedures in which
infectious aerosols or splashes may be created are conducted in BSCs or other
physical containment equipment.
Example: HIV
Biohazard Level 3 Work with indigenous or exotic agents that may cause serious or lethal disease via
aerosol transmission (inhalation).
Strictly controlled and must be registered with government agencies
Ventilation must provide ducted, directional airflow with no re-circulation; must
have all BL-2 protestions plus more protective primary barriers as necessary
(scrub suits, respirators, etc).
Examples: West Nile, Yellow Fever
Biohazard Level 4 Work with agents that are extremely dangerous and pose a high risk of life-
threatening disease.
Maximum protection and containment. Complete clothing
change/shower/decontamination of all meterials before leaving the facility. Class
III Biosafety cabinet.
Usually work is done in separate, isolated buildings with dedicated supply/exhaust
ventilation through HEPA filters.
Examples: Ebola, Lassa Virus, or any agent with unknown risks of transmission.