,
, CHM2046L FINAL EXAM UF SPRING 2021
UNIVERSITY OF FLORIDA CHEMISTRY 2 LAB REVIEW
& PRACTICE
beer-lambert law - CORRECT ANSWERS-A = εdc
A = absorbance
ε = molar absorptivity (L mol-1 cm-1)
d = distance the light travels through the solution (the cuvette) in cm
c = concentration of the solution (mol/L)
Beer's Law calibration curve:
Use the following data to generate a calibration curve of Absorbance vs
Concentration at λmax = 420.6 nm.
concentration 1: 6e-5 M; absorbance 1: 1.330
concentration 2: 2e-5 M; absorbance 1: 0.451
concentration 1: 1e-5 M; absorbance 1: 0.239
concentration 1: 2e-6 M; absorbance 1: 0.042