DETAILED EXPLANATIONS 2026
◉ Genetic Material Hypothesis.
Answer: Proteins were thought to carry genetic information.
◉ Transformation.
Answer: Process of genetic material transfer between organisms.
◉ Transforming Factor.
Answer: Oswald Avery identified DNA as the transforming factor.
◉ Hershey-Chase Experiment.
Answer: Showed that DNA was genetic material
◉ Viral DNA Labeling.
Answer: DNA labeled with phosphorus, protein with sulfur.
◉ Phage DNA Entry.
Answer: Only viral DNA enters E. coli, not protein.
,◉ Chargaff's Rules.
Answer: A=T and C=G base pairing in DNA.
◉ Nucleotide Components.
Answer: Sugar, phosphate, and nitrogenous base.
◉ DNA Replication Primer.
Answer: Short RNA segment initiates DNA synthesis.
◉ Leading Strand.
Answer: Continuously synthesized in the 5' to 3' direction.
◉ Lagging Strand.
Answer: Discontinuously synthesized in the 3' to 5' direction with
Okazaki fragments.
◉ Okazaki Fragments.
Answer: Short DNA segments on the lagging strand.
◉ Nucleotide Addition Direction.
Answer: 5' to 3' direction
, ◉ DNA Replication Enzymes.
Answer: Helicase
Polymerase
Ligase
Primase
Topoisomerase
Single-Strand Binding Protein
◉ Helicase.
Answer: Unwinds the DNA double helix during replication.
◉ DNA Polymerase.
Answer: Synthesizes new DNA strands from nucleotides.
◉ Ligase.
Answer: Joins Okazaki fragments on the lagging strand.
◉ Primase.
Answer: Synthesizes RNA primers for DNA replication.
◉ Single-Strand Binding Proteins.
Answer: Stabilize unwound DNA during replication.