BIO 121 LAB 1 COMPREHENSIVE STUDY
GUIDE 2026 MICROSCOPY AND CELLS
SOLUTIONS VERIFIED A+
◉ Revolving Turret.
Answer: Holds 4 objective lens
◉ Objective Lens.
Answer: Low - 4x magnification --> 40x with ocular lens
Medium - 10x " -->100x "
High - 40x " --> 400x "
Oil Immersion - 100x Not Used
◉ Stage.
Answer: Moveable platform where slide is
placed
◉ Slide/Stage Adjustment Knobs.
Answer: Adjusts up-and-down and side-to-
,side movement of the stage
◉ Light Source.
Answer: Illuminates the specimen
◉ Condenser.
Answer: Specialized lens that condenses the
light and focus it on the specimen
◉ Diaphragm.
Answer: Affects contrast by controlling the size of the hole that the
light passes through
◉ Condenser Adjustment Knob.
Answer: Moves the condenser; closer to the stage will illuminate the
specimen more brightly, further away will lower the light level
◉ Slide Holding Arm.
Answer: Secures the slide to the stage
◉ Illumination Control.
Answer: Increases or decreases the level of illumination
, ◉ Coarse focus knob.
Answer: Makes coarse adjustment to the focus
Use with low power ONLY
◉ Fine focus knob.
Answer: Makes fine adjustment to the focus
Use with medium and high power ONLY
◉ 1 mm equivalents.
Answer: 1000 micrometers (um)
1000000 nanometers (nm)
◉ Resolution.
Answer: The ability to clearly separate details of an image
◉ Limit of resolution for light microscope.
Answer: 500 micrometers
◉ Techniques to make a wet mount.
Answer: 45 degree angle of cover slip
GUIDE 2026 MICROSCOPY AND CELLS
SOLUTIONS VERIFIED A+
◉ Revolving Turret.
Answer: Holds 4 objective lens
◉ Objective Lens.
Answer: Low - 4x magnification --> 40x with ocular lens
Medium - 10x " -->100x "
High - 40x " --> 400x "
Oil Immersion - 100x Not Used
◉ Stage.
Answer: Moveable platform where slide is
placed
◉ Slide/Stage Adjustment Knobs.
Answer: Adjusts up-and-down and side-to-
,side movement of the stage
◉ Light Source.
Answer: Illuminates the specimen
◉ Condenser.
Answer: Specialized lens that condenses the
light and focus it on the specimen
◉ Diaphragm.
Answer: Affects contrast by controlling the size of the hole that the
light passes through
◉ Condenser Adjustment Knob.
Answer: Moves the condenser; closer to the stage will illuminate the
specimen more brightly, further away will lower the light level
◉ Slide Holding Arm.
Answer: Secures the slide to the stage
◉ Illumination Control.
Answer: Increases or decreases the level of illumination
, ◉ Coarse focus knob.
Answer: Makes coarse adjustment to the focus
Use with low power ONLY
◉ Fine focus knob.
Answer: Makes fine adjustment to the focus
Use with medium and high power ONLY
◉ 1 mm equivalents.
Answer: 1000 micrometers (um)
1000000 nanometers (nm)
◉ Resolution.
Answer: The ability to clearly separate details of an image
◉ Limit of resolution for light microscope.
Answer: 500 micrometers
◉ Techniques to make a wet mount.
Answer: 45 degree angle of cover slip
