1. All of the following are used to lyse cells EXCEPT
Mild detergents
Sonication
Electrophoresis
Enzymes
Mechanical disruption
2. Which of the following is not a component of peptidoglycan?
N-acetylglucosamine
N-acetylmuramic acid
Peptide cross-links
Lipopolysaccharide
Amino acid
3. What is an extension of the cytoplasm that attaches bacteria to a surface called?
Fimbrium
Pilus
Nanotube
Stalk
Flagellum
4. Microbes include members of which of the following groups?
Bacteria, Archaea, protozoa, plants
Bacteria, Archaea, fungi, viruses
Archaea, viruses, plants, fungi
Bacteria, animal, protozoa, fungi.
5. Which best defines a living organism that lives inside a larger organism?
Extremophile
Endosymbiont
, Virus
Organelle
Biofilm
6. Which of the following scientists did not contribute to the discovery of the identity
or structure of the genetic material
Rosalind Franklin
Lynn Margulis
Francis Crick
James Watson
7. Which of the following statements about the size of microbes is false?
Prokaryotic microbes tend to have a size less than 10um
A few bacterial species are large enough to be seen by the unaided eye
Choose this answer if all others are true.
Eukaryotic microbes tend to have a size of 10-100um
8. In Chemotaxis, CW flagellar rotation occurs in response to a repellant stimulating
membrane bound receptors.
True
False
9. This scientist discovered the microbial basis for fermentation
Louis Pasteur
Rosalind Franklin
Robert Koch
Edward Jenner
10. This doctor ordered other doctors to wash their hands with chlorine, an
antiseptic agent, which resulted in a decrease in the obstetric mortality rate.
James Watson
Lady Mary Montagu
Ignaz Semmelweis
, Joseph Lister
Exam 1
1. Which of the following is the correct order of a typical bacterial batch growth
curve.
a. Log ->lag->stationary->death
b. lag->log->stationary->death
c. lag ->stationary->log->death
d. log->sationary->lag->death
2. Which of the follow statements about the history of microbial control is
TRUE?
a. Lister discovered antibiotics
b. Fleming developed antiseptics
c. Both A and B
d. Neither A nor B
3. Viruses are:
a. Infectious agents that infect multi-cellular organisms
b. Noncellular particles that take over the metabolism of a cell to
generate more virus particles
c. Pathogen that replicate in complex growth media
d. Cellular particles that belong to the archaea domain
e. Microbes that consists of lipid membrane enclosed genomes
4. The first cellular genomes to be sequenced were those of:
a. Humans
b. Bacteria
c. Viruses
d. Prions
e. fungi
5. Koch’s postulates include all the following EXCEPT
a. A pure culture of the pathogen must be obtained
b. The pathogen must be found in every individual suffering from the
disease
c. The isolated pathogen must be used to infect healthy hosts
d. The pathogen must be shown to contain toxins
e. Healthy individuals infected with the isolated suspected pathogens
must come down with the disease.
6. A cafeteria worker who fails to wash his hands thoroughly and fails to wear
gloves inoculates a quiche with four E.coli when he uses his finger to test
whether it is done. By the time you purchase the quiche, there are 1024 e.coli
cells in it. How many generations did the cells go through?
a. 4
b. 5
c. 8
d. 32
e. 64
, 7. Microbes can shape human history via:
a. Lithotrophic activities
b. Production of alcoholic beverages
c. Diseases that they cause
d. Production of cheese
e. All of the above
8. The uses of agar as the gelling agent in solid media was suggested by:
a. Robert Koch
b. Ignaz Semmelweis
c. Angelina Hesse
d. Louis Pasteur
e. Richard Petri
9. What was the basis for the smallpox vaccine introduced by Edward jenner?
a. Chickenpox virus
b. Cowpox virus
c. Rabies virus
d. Smallpox virus
e. anthrax
10. Which of the orientated of the division plan – cell-grouping combinations
listed below is (are) TRUE?
a. Division in two perpendicular plans – strepto
b. Division in parallel plans – staphylo
c. Division in random plans – tetrads
d. Two of the above
e. Non of the above
11. A ball-shapes microbes is referred to as a:
a. Bacillus
b. Coccus
c. Vibrio
d. Stepto
e. spirochete
12. Human pathogens are ______.
a. Halophiles
b. Mesophiles
c. Psychrophiles
d. Thermophiles
e. extremophiles
13. Staining helps to visualize bacteria by:
a. Increasing the size of the cell
b. Increasing the mortality of the cell
c. Increasing the contrast of the image
d. Increasing the magnification of the image
e. Increasing the aberration of the image
14. Which of the following would be most appropriate to visualize viral particles
being assembled insides an infected bacterial cell
a. Dark field microcopy