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NUTR 405 Complete A+ Study guide_ Fall 2022 Proximate Analysis Lecture 1. List the five proximate components, their importance to food analysts, and the various methods/apparatus that each can be determined. **just the methods/apparatus that we covered in lab-not the numerous other methods of analysis that are covered in the text book Proximate Analysis: quantitative analysis of any food to determine the percentage of its components • Determine moisture, ash, fat, protein and carbohydrate content of a food • Conducted with official methods – Association of Official Analytical (AOAC), American Association for Clinical Chemistry (AACC), American Oil Chemists Society (AOCS) Moisture: food item – moisture = total solids – natural food (~70%), fruits and veggies (~95%) • Role of water in food: texture – either the presence or absence of water • States of water in food: free water, bound water, adsorbed • Relevance of measuring water: determine food quality, food stability and preservation, microbial stability, legal requirements/labeling (QC and QA, flour 12-15%), economic • Method to determine: oven drying (vacuum or convection ovens), freeze drying (sublimation) • How to calculate: (amount lost/original wet weight) * 100 = % moisture Fat: relevance of measuring lipids – nutritional information/labeling, lipid oxidation, physical characteristics of foods, food quality • Methods to determine fat content: 1. Goldfisch – continuous, gold standard, food analysis 2. Soxhlet – semi continuous 3. Mojonnier – discontinuous • How to calculate: (weight of lipid extracted/sample weight) * 100 = % fat Protein: relevance of measuring protein – determine total protein/individual AAs, investigate functional properties, nutrition labeling • Methods to determine protein content: 1. Kjeldahl – gold standard 2. Dumas – combustion reduction purification detection, much faster than kjeldahl (4min v. 1-2hrs) • Actually measuring nitrogen – nitrogen conversion factor (5.3) 3. Bradford – not very conclusive, can be time consuming Ash: ash content v. mineral content, relevance of measuring ash – nutrition labeling, quality of raw products, microbial stability, nutrition, processing • Method to determine ash content: 1. Wet ashing – strong acids + heat 2. Dry ashing – muffle furnace (500-600 C for 24 hours (AOAC) • How to calculate: (dry weight/original wet weight) * 100 = % ash Carbohydrate: classification of CHOs, sweetness appearance, texture • Relevance of measuring CHOs – nutritional labeling, standards of identity, food quality, economic • Methods to determine carbohydrate content: 1. Indirect measurement – total % carbohydrate by difference (100 – (%fat + %protein + %ash + % h20 in 100 g food)) 2. Direct measurement – sum of weight of individual CHO + fiber via • Chromatographic + electrophoretic methods • Chemical methods • Titration methods • Enzymatic methods Equipment Overview 1. Recognize the best applications of each apparatus/instrument 1. Instrument & Manufacturer: • 1.A Precision Vacuum Oven – quantitative analysis of any food to determine the percent of 5 constituents • 1.B Precision Scientific Thelco Convection Oven • 1.C LabConco Freezone 4.5 Freeze Dryer ..............................Continue

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Recent door jou bekeken

Waarom studenten kiezen voor Stuvia

Gemaakt door medestudenten, geverifieerd door reviews

Kwaliteit die je kunt vertrouwen: geschreven door studenten die slaagden en beoordeeld door anderen die dit document gebruikten.

Niet tevreden? Kies een ander document

Geen zorgen! Je kunt voor hetzelfde geld direct een ander document kiezen dat beter past bij wat je zoekt.

Betaal zoals je wilt, start meteen met leren

Geen abonnement, geen verplichtingen. Betaal zoals je gewend bent via iDeal of creditcard en download je PDF-document meteen.

Student with book image

“Gekocht, gedownload en geslaagd. Zo makkelijk kan het dus zijn.”

Alisha Student

Bezig met je bronvermelding?

Maak nauwkeurige citaten in APA, MLA en Harvard met onze gratis bronnengenerator.

Bezig met je bronvermelding?

Veelgestelde vragen