2022
Biosafety
MAASTRICHT UNVERSITY
,Table of Contents
LabBuddy........................................................................................................................................2
Case study 1...........................................................................................................................................2
Collecting human samples.....................................................................................................................................2
Transport to the lab................................................................................................................................................2
Entering lab...........................................................................................................................................................2
Collect plasma.......................................................................................................................................................3
Dilute sputum........................................................................................................................................................4
Cleaning up............................................................................................................................................................4
Leaving the lab......................................................................................................................................................4
Case study 2...........................................................................................................................................5
GMO......................................................................................................................................................................5
Isolate gene of interest...........................................................................................................................................5
Introduction....................................................................................................................................5
Creating viral plasmids..........................................................................................................................................6
Incident..................................................................................................................................................................7
Cleaning up spill....................................................................................................................................................8
Fixing the incubator...............................................................................................................................................9
Production of adenovirus......................................................................................................................9
Safety consideration..............................................................................................................................9
Disinfectants..........................................................................................................................................................9
Biological safety cabinet.......................................................................................................................................9
Protect your experiment......................................................................................................................................10
Production of adenovirus – protocol...................................................................................................................10
Infect cells with adenovirus................................................................................................................10
Protocol................................................................................................................................................................10
Final remarks.......................................................................................................................................12
Lesson 1 - 18/11/22.......................................................................................................................12
Biological agents..................................................................................................................................12
Chain of infection................................................................................................................................................12
Aereosol...............................................................................................................................................................12
Risk assessment...................................................................................................................................................12
Common safety rules...........................................................................................................................................12
Preventive measures............................................................................................................................................13
Biosafety cabinet.................................................................................................................................................13
Decontamination..................................................................................................................................................13
Waste handling....................................................................................................................................................13
Genetic modified organisms................................................................................................................14
Genetic modification...........................................................................................................................................14
Risks....................................................................................................................................................................14
Legislation...........................................................................................................................................................14
Conclusion...........................................................................................................................................................14
Summary..............................................................................................................................................14
2 lessons + digital Q and A + labBuddy exam almost on LabBuddy biosafety certificate
1
, LabBuddy
Case study 1
Collecting human samples
Collection of blood and sputum sample + transportation of them.
which substances are considered as biological agents ?
o cell cultures
o microorganisms
o genetically modified microorganisms
o human endoparasites
which disease can they cause?
o Infection
o Allergy
o Stomach and intestinal complaints
Biological agents can be present in blood and sputum samples diseases that can be passed to other
humans.
Biological agent Possibly present in Route of transmission
HIV Blood Blood/blood contact, mucous membranes
Hepatitis B Blood Blood/blood contact, mucous membranes
Hepatitis C Blood Blood/blood contact, mucous membranes
Influenza Sputum Inhalation
Tuberculosis Sputum Inhalation
Corona virus Sputum Inhalation
E. coli - Ingestion
Salmonella - Ingestion
Transport to the lab
Internal transport = buildings are connected via a walkway
Sample preparation for the internal transport:
1. the outside of the sample tube/container needs to be disinfected with a suitable disinfectant (es.
70% ethanol)
2. the samples should be double contained for transport in a leak-proof, unbreakable and
disinfectable containe
a. tube + container = the first containment
b. the second = container that complies with the requirements
3. the outside of the second container should be labeled with a biohazard sticker
The samples are then ready for internal transport.
Entering lab
You are about to analyse biological agents: blood and sputum.
2
Biosafety
MAASTRICHT UNVERSITY
,Table of Contents
LabBuddy........................................................................................................................................2
Case study 1...........................................................................................................................................2
Collecting human samples.....................................................................................................................................2
Transport to the lab................................................................................................................................................2
Entering lab...........................................................................................................................................................2
Collect plasma.......................................................................................................................................................3
Dilute sputum........................................................................................................................................................4
Cleaning up............................................................................................................................................................4
Leaving the lab......................................................................................................................................................4
Case study 2...........................................................................................................................................5
GMO......................................................................................................................................................................5
Isolate gene of interest...........................................................................................................................................5
Introduction....................................................................................................................................5
Creating viral plasmids..........................................................................................................................................6
Incident..................................................................................................................................................................7
Cleaning up spill....................................................................................................................................................8
Fixing the incubator...............................................................................................................................................9
Production of adenovirus......................................................................................................................9
Safety consideration..............................................................................................................................9
Disinfectants..........................................................................................................................................................9
Biological safety cabinet.......................................................................................................................................9
Protect your experiment......................................................................................................................................10
Production of adenovirus – protocol...................................................................................................................10
Infect cells with adenovirus................................................................................................................10
Protocol................................................................................................................................................................10
Final remarks.......................................................................................................................................12
Lesson 1 - 18/11/22.......................................................................................................................12
Biological agents..................................................................................................................................12
Chain of infection................................................................................................................................................12
Aereosol...............................................................................................................................................................12
Risk assessment...................................................................................................................................................12
Common safety rules...........................................................................................................................................12
Preventive measures............................................................................................................................................13
Biosafety cabinet.................................................................................................................................................13
Decontamination..................................................................................................................................................13
Waste handling....................................................................................................................................................13
Genetic modified organisms................................................................................................................14
Genetic modification...........................................................................................................................................14
Risks....................................................................................................................................................................14
Legislation...........................................................................................................................................................14
Conclusion...........................................................................................................................................................14
Summary..............................................................................................................................................14
2 lessons + digital Q and A + labBuddy exam almost on LabBuddy biosafety certificate
1
, LabBuddy
Case study 1
Collecting human samples
Collection of blood and sputum sample + transportation of them.
which substances are considered as biological agents ?
o cell cultures
o microorganisms
o genetically modified microorganisms
o human endoparasites
which disease can they cause?
o Infection
o Allergy
o Stomach and intestinal complaints
Biological agents can be present in blood and sputum samples diseases that can be passed to other
humans.
Biological agent Possibly present in Route of transmission
HIV Blood Blood/blood contact, mucous membranes
Hepatitis B Blood Blood/blood contact, mucous membranes
Hepatitis C Blood Blood/blood contact, mucous membranes
Influenza Sputum Inhalation
Tuberculosis Sputum Inhalation
Corona virus Sputum Inhalation
E. coli - Ingestion
Salmonella - Ingestion
Transport to the lab
Internal transport = buildings are connected via a walkway
Sample preparation for the internal transport:
1. the outside of the sample tube/container needs to be disinfected with a suitable disinfectant (es.
70% ethanol)
2. the samples should be double contained for transport in a leak-proof, unbreakable and
disinfectable containe
a. tube + container = the first containment
b. the second = container that complies with the requirements
3. the outside of the second container should be labeled with a biohazard sticker
The samples are then ready for internal transport.
Entering lab
You are about to analyse biological agents: blood and sputum.
2
