ASCP EXAM NEWEST 2023 REAL EXAM 200
QUESTIONS AND CORRECT DETAILED ANSWERS
|AGRADE
PCR that is not a limitation of real-time PCR?
Select one:
a. Requirement of a large initial sample
b. Inability to quantitate DNA product due to endpoint analysis only
c. Ability to detect PCR product during the plateau stage - ANSWER- B. Inability
to quantitate DNA product due to endpoint analysis only
A patient has the following test results:ANA = positive 1:320RA =
positiveComplement = decreasedThe above results would be seen in patients with:
Select one:
a. Rheumatic fever
b. Rheumatoid arthritis
c. Lupus Erythematosus
d. Glomerulonephritis - ANSWER- C. Lupus Erythmatosus
A 40-year-old female receives two units of Red Blood Cells during a surgical
procedure. The patient has no prior history of transfusions. Seven days later, she
presents with extensive bruising of the extremities and bleeding of the gums, with
no additional symptoms. Her platelet count is 5X109/L (reference interval 150-400
X 109 /L). What is the most likely diagnosis?
Select one:
a. Post transfusion purpura (PTP)
b. Acute hemolytic transfusion reaction (AHTR)
c. Disseminated intravascular coagulation (DIC)
d. Allergic reaction - ANSWER- A. Post transfusion purpura (PTP)
Approximately what percent of the overall population is Rh negative:
Select one:
a. 10%
b. 15%
c. 25%
d. 35% - ANSWER- B. 15%
Which one of the following statements concerning endocytosis is NOT TRUE?
,Select one:
a. Cells may employ endocytosis to ingest large polar molecules (macromolecules)
from extracellular fluid.
b. In endocytosis, the cell membrane often extends outward until it surrounds the
material that will be ingested.
c. Endocytosis is a natural process of intracellular digestion for nutrition.
d. Endocytosis is the process by which phagosomes are released by the cell to
expel the macromolecules inside. - ANSWER- D. Endocytosis is the process by
which phagosomes are released by the cell to expel the macromolecules inside
Which of the following represents the approximate percentage of the population
that is Rh positive:
Select one:
a. 35%
b. 65%
c. 85%
d. 95% - ANSWER- C. 85%
Which of these nitrogenous bases is present in deoxyribonucleic acid (DNA), but
not in ribonucleic acid (RNA)?
Select one:
a. Guanine
b. Cytosine
c. Adenine
d. Thymine - ANSWER- D. Thymine
Which real-time PCR quantification method is only effective if the amplification
efficiency of both target and reference samples are equal?
Select one:
a. Standard Curve Method
b. Comparative Threshold Method - ANSWER- B. Comparative Threshold
Method
Given the following results, what is the immune status of the patient?HBsAg:
negativeHBeAg: negativeAnti-HBc: positiveAnti-HBs: positive
Select one:
a. acute infection
b. chronic infection
c. recovery
d. immunization - ANSWER- C. Recovery
,An O pos patient has no reactions at immediate spin but both screen cells are
positive and all antibody panel cells are reacting 1+ at AHG. The auto control is
1+. What would you suspect to be the cause?
Select one:
a. Cold autoantibody
b. Warm autoantibody - ANSWER- Warm autoantibody
Which of these is NOT a Systemic Autoimmune Rheumatic Disease (SARD)?
Select one:
a. Systemic sclerosis
b. Lupus erythematosus
c. Rheumatoid arthritis
d. Multiple sclerosis - ANSWER- D. Multiple Sclerosis
Which of the following is NOT true about hybridization in PCR procedures?
Select one:
a. Hybridization cannot happen between a strand of DNA and a strand of RNA
b. Hybridization is the process of single strands of nucleic acid bonding together to
form double strands
c. Hybridization can also be called annealing
d. Complementary bases link together with hydrogen bonds - ANSWER- A.
Hybridization cannot happen between a strand of DNA and a strand of RNA
Which is the first marker (antigen or antibody) which will become positive after
exposure to Hepatitis B:
Select one:
a. HBsAg
b. Anti-HBs
c. Anti-HBe
d. Anti-HBc - ANSWER- A. HBsAg
Monoclonal antibodies are usually manufactured in vitro by using:
Select one:
a. Cultured T cells
b. Human plasma cells
c. Hybridomas
d. Cytotoxic T cells - ANSWER- C. Hydridomas
, During routine inspection, a unit of unexpired blood was noticed to have a black
color with numerous small clots. What is the likely cause for this observation?
Select one:
a. the unit was frozen
b. donor had DIC
c. viral contamination
d. bacterial contamination - ANSWER- D. Bacterial Contamination
Given the following results, what is the immune status for the patient?HbsAg:
positiveHbeAg: positiveAnti-HBc IgM: positiveAnti-HBs: negative
Select one:
a. acute infection
b. chronic infection
c. immunization
d. susceptible - ANSWER- A. Acute infection
Twelve weeks after onset of the disease, patients with uncomplicated acute
hepatitis B usually will demonstrate which of the following in their serum?
Select one:
a. HBsAg
b. Anti-HTLV
c. Anti-HBe
d. Anti-HIV - ANSWER- C. Anti-HBe
While ABO, Rh, Kell, Duffy, Kidd, SsU are clinically significant, which of the
following antibodies generally react at ONLY the Coombs phase (also knowns as
Antihuman Globulin phase):
Select one:
a. Rh, Kell
b. ABO
c. Kell, Duffy, Kidd
d. SsU, Kidd - ANSWER- C. Kell, Duffy, Kidd
Platelets should be stored at what temperature:
Select one:
a. 1-6 degrees Celsius
b. 10-16 degrees Celsius
c. 20-24 degrees Celsius
d. 34-37 degrees Celsius - ANSWER- C. 20-24 degrees Celsius
QUESTIONS AND CORRECT DETAILED ANSWERS
|AGRADE
PCR that is not a limitation of real-time PCR?
Select one:
a. Requirement of a large initial sample
b. Inability to quantitate DNA product due to endpoint analysis only
c. Ability to detect PCR product during the plateau stage - ANSWER- B. Inability
to quantitate DNA product due to endpoint analysis only
A patient has the following test results:ANA = positive 1:320RA =
positiveComplement = decreasedThe above results would be seen in patients with:
Select one:
a. Rheumatic fever
b. Rheumatoid arthritis
c. Lupus Erythematosus
d. Glomerulonephritis - ANSWER- C. Lupus Erythmatosus
A 40-year-old female receives two units of Red Blood Cells during a surgical
procedure. The patient has no prior history of transfusions. Seven days later, she
presents with extensive bruising of the extremities and bleeding of the gums, with
no additional symptoms. Her platelet count is 5X109/L (reference interval 150-400
X 109 /L). What is the most likely diagnosis?
Select one:
a. Post transfusion purpura (PTP)
b. Acute hemolytic transfusion reaction (AHTR)
c. Disseminated intravascular coagulation (DIC)
d. Allergic reaction - ANSWER- A. Post transfusion purpura (PTP)
Approximately what percent of the overall population is Rh negative:
Select one:
a. 10%
b. 15%
c. 25%
d. 35% - ANSWER- B. 15%
Which one of the following statements concerning endocytosis is NOT TRUE?
,Select one:
a. Cells may employ endocytosis to ingest large polar molecules (macromolecules)
from extracellular fluid.
b. In endocytosis, the cell membrane often extends outward until it surrounds the
material that will be ingested.
c. Endocytosis is a natural process of intracellular digestion for nutrition.
d. Endocytosis is the process by which phagosomes are released by the cell to
expel the macromolecules inside. - ANSWER- D. Endocytosis is the process by
which phagosomes are released by the cell to expel the macromolecules inside
Which of the following represents the approximate percentage of the population
that is Rh positive:
Select one:
a. 35%
b. 65%
c. 85%
d. 95% - ANSWER- C. 85%
Which of these nitrogenous bases is present in deoxyribonucleic acid (DNA), but
not in ribonucleic acid (RNA)?
Select one:
a. Guanine
b. Cytosine
c. Adenine
d. Thymine - ANSWER- D. Thymine
Which real-time PCR quantification method is only effective if the amplification
efficiency of both target and reference samples are equal?
Select one:
a. Standard Curve Method
b. Comparative Threshold Method - ANSWER- B. Comparative Threshold
Method
Given the following results, what is the immune status of the patient?HBsAg:
negativeHBeAg: negativeAnti-HBc: positiveAnti-HBs: positive
Select one:
a. acute infection
b. chronic infection
c. recovery
d. immunization - ANSWER- C. Recovery
,An O pos patient has no reactions at immediate spin but both screen cells are
positive and all antibody panel cells are reacting 1+ at AHG. The auto control is
1+. What would you suspect to be the cause?
Select one:
a. Cold autoantibody
b. Warm autoantibody - ANSWER- Warm autoantibody
Which of these is NOT a Systemic Autoimmune Rheumatic Disease (SARD)?
Select one:
a. Systemic sclerosis
b. Lupus erythematosus
c. Rheumatoid arthritis
d. Multiple sclerosis - ANSWER- D. Multiple Sclerosis
Which of the following is NOT true about hybridization in PCR procedures?
Select one:
a. Hybridization cannot happen between a strand of DNA and a strand of RNA
b. Hybridization is the process of single strands of nucleic acid bonding together to
form double strands
c. Hybridization can also be called annealing
d. Complementary bases link together with hydrogen bonds - ANSWER- A.
Hybridization cannot happen between a strand of DNA and a strand of RNA
Which is the first marker (antigen or antibody) which will become positive after
exposure to Hepatitis B:
Select one:
a. HBsAg
b. Anti-HBs
c. Anti-HBe
d. Anti-HBc - ANSWER- A. HBsAg
Monoclonal antibodies are usually manufactured in vitro by using:
Select one:
a. Cultured T cells
b. Human plasma cells
c. Hybridomas
d. Cytotoxic T cells - ANSWER- C. Hydridomas
, During routine inspection, a unit of unexpired blood was noticed to have a black
color with numerous small clots. What is the likely cause for this observation?
Select one:
a. the unit was frozen
b. donor had DIC
c. viral contamination
d. bacterial contamination - ANSWER- D. Bacterial Contamination
Given the following results, what is the immune status for the patient?HbsAg:
positiveHbeAg: positiveAnti-HBc IgM: positiveAnti-HBs: negative
Select one:
a. acute infection
b. chronic infection
c. immunization
d. susceptible - ANSWER- A. Acute infection
Twelve weeks after onset of the disease, patients with uncomplicated acute
hepatitis B usually will demonstrate which of the following in their serum?
Select one:
a. HBsAg
b. Anti-HTLV
c. Anti-HBe
d. Anti-HIV - ANSWER- C. Anti-HBe
While ABO, Rh, Kell, Duffy, Kidd, SsU are clinically significant, which of the
following antibodies generally react at ONLY the Coombs phase (also knowns as
Antihuman Globulin phase):
Select one:
a. Rh, Kell
b. ABO
c. Kell, Duffy, Kidd
d. SsU, Kidd - ANSWER- C. Kell, Duffy, Kidd
Platelets should be stored at what temperature:
Select one:
a. 1-6 degrees Celsius
b. 10-16 degrees Celsius
c. 20-24 degrees Celsius
d. 34-37 degrees Celsius - ANSWER- C. 20-24 degrees Celsius
