Speceroscopy
SpecevoScopY dedis wie the neetactlon of atoms
or moleau les or matey with eecere mag netic 7ad iato.
UV-vi'sible SpeceroscopY CPmciple)
-WSble Spectro Stopy oresponds toprettron fc trans
fam
On of moleules that absorb! energy n ehe
Wor visiele lighe
ins erumen tation.
pHYACHO e bv
Garn
Lignt Seurae isible
MMr4 ReFeveno
Cuveee
Pebeciey 2
REGATER
Bea
Ha
MYD
2
Sarnpe
Petecv4
Mimerg Bean
A beam of ng int
9plit i9 in to to equal beams and
PaSsed hrougn Sam pe
oneis
c beam) and the Oer
eOg
Yeerene
CbeamT).The ntensieles o #hese lgnt
beams
a e chen Mas uredtY elecon ic detethOrs ard opavea, 1
ere Som p dves nee absarb lignt 0+ ql Ven uawelvgth tenT To
4Sample asorbr igot thn EiS IeSs tad To. and this
differonce Bdctece retded.
, Chemis
Tere are 4 tranSi tlons in bv spectrseopy.
Lg-o
This type o4 erangiion taKes place when
eleceon is pro mored to ane'bon ding " orbita
Bg: Sacura ted Hydro car bon cczHs)
2. n - g "
Non- banding 7 elec6ron is promo red t antibendig
" or bital eg: errnanol
3.T-*
is bype o transition corres ponds cne promoNan
O ret0 mti bonding *orblea
unsa turated hydrotar bm, etherye
o an anbo
Hee a hon bendiog né is PYomaced
ndina * Tbitai. eg: phenol.
Show UV
moa celes
ChEch
o t h e Sslloan
visebla absotptoh Spectram
bene
methane
4e
TTpe bona
no: loube bpn
S bod
d) Cyio hoxana
Au
maecule wil absorb at longese wave lenqth
. wnigh
dave bond absorbS Yre uv.
AS. . Coviugated