Complete BOC Immunology (MLT only) Exam (Latest
2024/2025) Questions with Verified Answers
+Rationale/ Grade A Score Guide (Medical Laboratory
Technicians)
What are antinuclear antibody tests used for?
a. To determine sensitivities for microbiology
b. To diagnose anemias
c. To diagnose lupus erythematosus
d. To determine PCR analysis - ANSWERc
SLE patients often have:
a. High titers of antimitochondrial antibody
b. Decrease immunoglobulin levels
c. Hight tiers of anti-smooth muscle antibodies
d. High tiers of DNA antibody - ANSWERd
Rheumatoid factor reacts with:
a. Latex particles coated with an inert substance
b. Kinetoplasts of Crithidia luciliae
c. Gamma globulin-coated particle
d. Rh positive erythrocytes - ANSWERc
Positive rheumatoid factor is generally associated with:
a. Anemia
b. Hyperglobulinemia
c. Azotemia
d. Decreased ESR - ANSWERb
Rheumatoid factors are defined as:
a. Antigens found in patients with RA
b. Autoantibodies with specificity for the Fc portion of the immunoglobulin (IgG)
molecule
c. Antibodies that bind to circulating IgM antibodies
d. Are identical to the rheumatoid arthritis precipitin - ANSWERb
High titers of antimicrosomal antibodies are most often found in :
a. Rheumatoid arthritis
b. SLE
c. Hepatitis
d. Thyroid disease - ANSWERd
If an acute serum titer was 1:4 and the convalescent titer was 1:32, what is the best
interpretation?
a. The test should be repeated
, b. The results are inconclusive since the titer went up instead of down
c. The patient had a previous infection
d. The patient has an active infection - ANSWERd
What is the labeled antibody in the indirect immunofluorescence method of
antibody detection?
a. Complement
b. Anti-human immunoglobulin
c. Rheumatoid factor
d. Human anti-goat immunoglobulin - ANSWERd
A substrate is first exposed to a patient's serum, then after washing, anti-human
immunoglobulin labeled with a fluorochrome is added. What is this procedure
called?
a. Fluorescence inhibition
b. Direct fluorescence
c. Indirect fluorescence
d. Fluorescent quenching - ANSWERc
What is Avidity?
a. Degree of hemolysis
b. Titer of an antigen
c. Dilution of an antibody
d. Strength of a reacting antibody - ANSWERd
What is the visible serological reaction between soluble antigen and its specific
antibody called?
a. Opsonization
b. Agglutination
c. Precipitation
d. Sensitization - ANSWERc
Which area on the curve represents equivalence of precipitate - ANSWERc
Which area represents no precipitate formed due to antigen excess - ANSWERd
Which area represents prozone - ANSWERa
Which area shows where antibody antigen complexes have begun to form -
ANSWERb
The following pattern of agglutination was observed in an antibody titration:
Tube 1 2 3 4 5 6 7 8 9
1+ 2+ 4+ 4+ 3+ 3+ 2+ 1+ 0
What is the most likely cause of these reactions
a. Faulty pipetting
2024/2025) Questions with Verified Answers
+Rationale/ Grade A Score Guide (Medical Laboratory
Technicians)
What are antinuclear antibody tests used for?
a. To determine sensitivities for microbiology
b. To diagnose anemias
c. To diagnose lupus erythematosus
d. To determine PCR analysis - ANSWERc
SLE patients often have:
a. High titers of antimitochondrial antibody
b. Decrease immunoglobulin levels
c. Hight tiers of anti-smooth muscle antibodies
d. High tiers of DNA antibody - ANSWERd
Rheumatoid factor reacts with:
a. Latex particles coated with an inert substance
b. Kinetoplasts of Crithidia luciliae
c. Gamma globulin-coated particle
d. Rh positive erythrocytes - ANSWERc
Positive rheumatoid factor is generally associated with:
a. Anemia
b. Hyperglobulinemia
c. Azotemia
d. Decreased ESR - ANSWERb
Rheumatoid factors are defined as:
a. Antigens found in patients with RA
b. Autoantibodies with specificity for the Fc portion of the immunoglobulin (IgG)
molecule
c. Antibodies that bind to circulating IgM antibodies
d. Are identical to the rheumatoid arthritis precipitin - ANSWERb
High titers of antimicrosomal antibodies are most often found in :
a. Rheumatoid arthritis
b. SLE
c. Hepatitis
d. Thyroid disease - ANSWERd
If an acute serum titer was 1:4 and the convalescent titer was 1:32, what is the best
interpretation?
a. The test should be repeated
, b. The results are inconclusive since the titer went up instead of down
c. The patient had a previous infection
d. The patient has an active infection - ANSWERd
What is the labeled antibody in the indirect immunofluorescence method of
antibody detection?
a. Complement
b. Anti-human immunoglobulin
c. Rheumatoid factor
d. Human anti-goat immunoglobulin - ANSWERd
A substrate is first exposed to a patient's serum, then after washing, anti-human
immunoglobulin labeled with a fluorochrome is added. What is this procedure
called?
a. Fluorescence inhibition
b. Direct fluorescence
c. Indirect fluorescence
d. Fluorescent quenching - ANSWERc
What is Avidity?
a. Degree of hemolysis
b. Titer of an antigen
c. Dilution of an antibody
d. Strength of a reacting antibody - ANSWERd
What is the visible serological reaction between soluble antigen and its specific
antibody called?
a. Opsonization
b. Agglutination
c. Precipitation
d. Sensitization - ANSWERc
Which area on the curve represents equivalence of precipitate - ANSWERc
Which area represents no precipitate formed due to antigen excess - ANSWERd
Which area represents prozone - ANSWERa
Which area shows where antibody antigen complexes have begun to form -
ANSWERb
The following pattern of agglutination was observed in an antibody titration:
Tube 1 2 3 4 5 6 7 8 9
1+ 2+ 4+ 4+ 3+ 3+ 2+ 1+ 0
What is the most likely cause of these reactions
a. Faulty pipetting
