PCB4233 Immunology Final Exam Questions and Correct Answers Latest Update 2025 (Rated A+)
RNA seq - Answers
ICC - Answers
FACS - Answers
MTT Assay - Answers
Primary Cell culture - Answers ch. 7 notes
Repair/Ligation - Answers Ku70/Ku80: DNA end binding proteins that bind to break and recruit DNA DPK
DNA dependent protein kinase: DS DNA repair enzyme (defective in SCID mice) activates artemis
Artemis: endonuclease opens hairpin loop
DNA ligase IV
(common DNA repair machinery present in all cells)
Variation - Answers although DS DNA breaks initiating V(D)J rearrangements are introduced precisely at
the junctions of signal sequences and coding sequences, the subsequent coding sequence joining is
imprecise
helps to generate additional antibody diversity by creating more hyper variability i the antigen binding
site
Combinstorial - Answers VDJ re combinations
multiple gene segments
Junctional flexibility - Answers removal or addition of nucleotide at time of joining Vs to Ds; Ds to Js and
Vs to Js- Artemis often cleaves hairpin loops asymmetrically so nucleotides have to be added
P region nucleotides - Answers caused by cutting of hairpin loop and the evening up
N region nucleotides - Answers up to 15 nucleotides at cut ends of V, D, J coding regions by
deoxynucleotidyl transferase
Somatic hypermutation - Answers
Combinatorial association of heavy and light chains - Answers when they combine it changes the antigen
being bound
Junctional Flexibility - Answers productive rearrangements
nonproductive rearrangements
RNA seq - Answers
ICC - Answers
FACS - Answers
MTT Assay - Answers
Primary Cell culture - Answers ch. 7 notes
Repair/Ligation - Answers Ku70/Ku80: DNA end binding proteins that bind to break and recruit DNA DPK
DNA dependent protein kinase: DS DNA repair enzyme (defective in SCID mice) activates artemis
Artemis: endonuclease opens hairpin loop
DNA ligase IV
(common DNA repair machinery present in all cells)
Variation - Answers although DS DNA breaks initiating V(D)J rearrangements are introduced precisely at
the junctions of signal sequences and coding sequences, the subsequent coding sequence joining is
imprecise
helps to generate additional antibody diversity by creating more hyper variability i the antigen binding
site
Combinstorial - Answers VDJ re combinations
multiple gene segments
Junctional flexibility - Answers removal or addition of nucleotide at time of joining Vs to Ds; Ds to Js and
Vs to Js- Artemis often cleaves hairpin loops asymmetrically so nucleotides have to be added
P region nucleotides - Answers caused by cutting of hairpin loop and the evening up
N region nucleotides - Answers up to 15 nucleotides at cut ends of V, D, J coding regions by
deoxynucleotidyl transferase
Somatic hypermutation - Answers
Combinatorial association of heavy and light chains - Answers when they combine it changes the antigen
being bound
Junctional Flexibility - Answers productive rearrangements
nonproductive rearrangements
