C HAPTER 5: M ICROBIAL G ROWTH AND ITS
C ONTROL
Test Bank for Brock Biology of Microorganism 16th Edition by Michael T .
Madigan
5.1 Multiple Choice Questions
1. Which of the following is/are transferred to daughter cells during bacterial
cell division?
A) onl y genomic DNA, the blueprint for everything the cell needs
B) individual genes and enz ymes to later be brought together to form the
genome
C) onl y the chromosome and enz ymes to immediatel y begin cellular
metabolism
D) chromosome, proteins, and all other cellular constituents
2. Bacterial growth refers to
A) an increase in the number of cells.
B) the occurrence of binary fission.
C) the time required for one cell to divide into two.
D) the occurrence of mitosis.
3. The time interval required for the formation of two cells from one is
called the
A) generation time.
B) growth time.
C) growth rate.
D) division rate.
,4. Turbidit y measurements are comm onl y utilized for monitoring
A) planktonic cultures.
B) biofilms.
C) conies.
D) sessile cultures.
5. The time between inoculation and the beginning of growth is usuall y
called the
A) lag phase.
B) log phase.
C) dormant phase.
D) death phase.
6. Optical densit y and viable cell concentration are LEAST proportional to
each other during the
A) lag phase.
B) exponential growth phase.
C) stationary phase.
D) death phase.
7. The optimal growth temperature of a bacterium is most closely related to
the optimal temperatu re for
A) transcription of DNA.
B) DNA replication.
C) enz yme function.
D) mRNA attachment to ribosomes.
8. What temperature is most commonl y used in autoclaves to sterilize growth
media and other devices prior to experimentation?
A) 95°C
B) 101°C
C) 121°C
, D) 140°C
9. Serial dilutions are generall y made
A) when the appropriate viable count is unknown.
B) based on powers of 10, although other serial dilution factors are
possible.
C) to reach a suitable dilution while minimizing error.
D) All of these answer cho ices appl y.
10.By controlling the concentration of nutrients continuousl y added to a
chemostat, cells can constantl y be maintained at the
A) exponential growth phase.
B) stationary growth phase.
C) lag growth phase.
D) death growth phase.
11.A common antimicrobial chemical (or chemicals) used as both an
antiseptic and a general disinfectant is
A) iodophors.
B) 70% alcohol.
C) hydrogen peroxide.
D) Each of these chemicals at appropriate concentrations can be used as
antiseptics o r disinfectants.
12.Selective medium differs from differ ential medium because
A) selective medium permits growth of more organisms than differential
medium.
B) selective medium permits growth of a particular microbial t ype while
differential medium is used to distinguish between t ypes of
organisms.
C) differential me dium differentiates pathogens from nonpathogens
while selective medium grows onl y pathogens.
C ONTROL
Test Bank for Brock Biology of Microorganism 16th Edition by Michael T .
Madigan
5.1 Multiple Choice Questions
1. Which of the following is/are transferred to daughter cells during bacterial
cell division?
A) onl y genomic DNA, the blueprint for everything the cell needs
B) individual genes and enz ymes to later be brought together to form the
genome
C) onl y the chromosome and enz ymes to immediatel y begin cellular
metabolism
D) chromosome, proteins, and all other cellular constituents
2. Bacterial growth refers to
A) an increase in the number of cells.
B) the occurrence of binary fission.
C) the time required for one cell to divide into two.
D) the occurrence of mitosis.
3. The time interval required for the formation of two cells from one is
called the
A) generation time.
B) growth time.
C) growth rate.
D) division rate.
,4. Turbidit y measurements are comm onl y utilized for monitoring
A) planktonic cultures.
B) biofilms.
C) conies.
D) sessile cultures.
5. The time between inoculation and the beginning of growth is usuall y
called the
A) lag phase.
B) log phase.
C) dormant phase.
D) death phase.
6. Optical densit y and viable cell concentration are LEAST proportional to
each other during the
A) lag phase.
B) exponential growth phase.
C) stationary phase.
D) death phase.
7. The optimal growth temperature of a bacterium is most closely related to
the optimal temperatu re for
A) transcription of DNA.
B) DNA replication.
C) enz yme function.
D) mRNA attachment to ribosomes.
8. What temperature is most commonl y used in autoclaves to sterilize growth
media and other devices prior to experimentation?
A) 95°C
B) 101°C
C) 121°C
, D) 140°C
9. Serial dilutions are generall y made
A) when the appropriate viable count is unknown.
B) based on powers of 10, although other serial dilution factors are
possible.
C) to reach a suitable dilution while minimizing error.
D) All of these answer cho ices appl y.
10.By controlling the concentration of nutrients continuousl y added to a
chemostat, cells can constantl y be maintained at the
A) exponential growth phase.
B) stationary growth phase.
C) lag growth phase.
D) death growth phase.
11.A common antimicrobial chemical (or chemicals) used as both an
antiseptic and a general disinfectant is
A) iodophors.
B) 70% alcohol.
C) hydrogen peroxide.
D) Each of these chemicals at appropriate concentrations can be used as
antiseptics o r disinfectants.
12.Selective medium differs from differ ential medium because
A) selective medium permits growth of more organisms than differential
medium.
B) selective medium permits growth of a particular microbial t ype while
differential medium is used to distinguish between t ypes of
organisms.
C) differential me dium differentiates pathogens from nonpathogens
while selective medium grows onl y pathogens.
