BIO 171 Lab exams 1-3
1. If you are working in a lab in which an autoclave is not available, and you
are pressed for time, which would you choose to best sterilize your
equipment? Hot steam or hot air? Explain why you chose your answer.: Hot
steam is the best choice as you can achieve a sterile environment in a matter of
minutes whereas hot air will take several hours to achieve the same effect.
2. What three elements are used in an autoclave to sterilize equipment?: Heat,
pressure, and steam.
3. What type of incubator is pictured below?: Fixed incubator
4. What is the minimum temperature an autoclave must be set at to achieve
sterile condition?: 125°C
5. At what temperature is the fixed incubator set to, as presented in the lab
video?: 37°C
6. What are the FOUR types of gloves presented in the lab video?: Latex,
Nitrile,
Thermal cold, Thermal heat
7. What are the main sections that should be found in a lab notebook? Name
at least 4.: Objective, Procedure, Notes, Results and Deviations
8. At what temperature should you refrigerate bacterial samples? Explain why
this is beneficial.: 4°C. This temperature slows bacterial growth and prolongs
the life of the sample.
9. What THREE rules were discussed in regards to lab safety that would
protect you and others from contamination?:
1. Never eat or drink in the lab
2. Always wear appropriate PPE
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1. If you are working in a lab in which an autoclave is not available, and you
are pressed for time, which would you choose to best sterilize your
equipment? Hot steam or hot air? Explain why you chose your answer.: Hot
steam is the best choice as you can achieve a sterile environment in a matter of
minutes whereas hot air will take several hours to achieve the same effect.
2. What three elements are used in an autoclave to sterilize equipment?: Heat,
pressure, and steam.
3. What type of incubator is pictured below?: Fixed incubator
4. What is the minimum temperature an autoclave must be set at to achieve
sterile condition?: 125°C
5. At what temperature is the fixed incubator set to, as presented in the lab
video?: 37°C
6. What are the FOUR types of gloves presented in the lab video?: Latex,
Nitrile,
Thermal cold, Thermal heat
7. What are the main sections that should be found in a lab notebook? Name
at least 4.: Objective, Procedure, Notes, Results and Deviations
8. At what temperature should you refrigerate bacterial samples? Explain why
this is beneficial.: 4°C. This temperature slows bacterial growth and prolongs
the life of the sample.
9. What THREE rules were discussed in regards to lab safety that would
protect you and others from contamination?:
1. Never eat or drink in the lab
2. Always wear appropriate PPE
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