Blood Banking (ASSESSMENT EXAM)
newest edition 2025//complete with
questions and correct answers
What is the disposition of a donor unit that contains an antibody?
A. The unit must be discarded.
B. Only the plasma can be used to make components.
C. The antibody must be absorbed from the unit.
D. Label the unit indicating that it contains antibody; release into inventory. - CORRECT
ANSWER D
Given situation where cells, crosshatch, auto control and DAT (anti-IgG) are all positive,
what procedure should be performed next?
A. Absorption using rabbit stroma
B. Antigen type the patients' cells
C. Elution followed by a cell panel on the elute
Decal panel - CORRECT ANSWER C
Can Rh positive blood can ever be transfused to a Rh negative person?
A. Never; under no circumstances
B. Only if Rh positive have been previously administered
C. May be transfused to males or females past childbearing age
D. Depends on the responder status of the individual - CORRECT ANSWER C
A crosshatch and screen cells are 2+ at immediate spin, 1+ at 37 C, and negative at the
IAT phase. Identify the problem.
A. Combination of antibodies
B. Cold antibodies
C. Rouleaux
D. Test error - CORRECT ANSWER B
What corrective action should be taken when rouleaux causes positive test results?
A. Wash cells and dilute serum
B. Perform an auto absorption
C. Run a panel
D. Perform an elution - CORRECT ANSWER A
,All of the following are the reasons for performing an absorption except:
A. Separation of mixtures of antibodies
B. Removal of interfering antibodies
C. Confirmation of weak antigen on red cells
D. Identification of antibodies causing positive DAT - CORRECT ANSWER D
What is needed for detection of some drug-induced antibodies?
A. Panel cells
B. Polyspecific Coombs sera
C. Enzyme-treated cells
D. Drug-coated cells - CORRECT ANSWER D
How can IgG antibodies be removed from red cells?
A. ZZAP
B. Cold elution
C. Thiol reagent
D. AET - CORRECT ANSWER A
How can IgM antibodies be separated from IgG antibodies?
A. Warm elution
B. Thiol reagents
C. Absorption with antigen-specific red cells
D. Impossible to separate - CORRECT ANSWER B
All of the condition below would require elution to identify antibodies coating the red
cells EXCEPT:
A. HDN
B. Hemolytic anemia
C. Multiple Myeloma
D. Transfusion reaction - CORRECT ANSWER C
In the IAT phase, what action should be taken if tubes are negative after addition of
check cells (reagent sensitized cells)?
A. Repeat the test; replace AHG reagent and check the cell washer.
B. Add additional check cells
C. Add additional AHG reagent
D. Record results as negative - CORRECT ANSWER All of the following would be used
to test the reactivity of AHG reagent except:
A. Diluted anti-K antisera
, B. Diluted anti-D antisera
C. Check cells
D. 6% albumin - CORRECT ANSWER A
What is the first step in the laboratory investigation of a transfusion reaction?
A. DAT on the post-transfusion sample
B. Check for clerical error
C. Repeat ABO and Rh typing of patient and donor unit.
D. Antibody screen on the post-transfusion sample. - CORRECT ANSWER B
A Trauma patient had a severe hemolytic reaction following a blood transfusion. What is
the most likely cause?
A. Immediate, no immunological: probably due to volume overload
B. Delayed Immunological: probably due to an antibody such as anti-Jk^a
C. Delayed no immunological: probably due to iron overload
D. Immediate, no immunological: probably due to clerical error, ABO incompatibility -
CORRECT ANSWER D
A patient has hemolytic reaction transfused 8 days ago. What is the most likely cause?
A. Immediate, no immunological: probably due to volume overload
B. Delayed Immunological: probably due to an antibody such as anti-Jk^a
C. Delayed no immunological: probably due to iron overload
D. Immediate, no immunological: probably due to clerical error, ABO incompatibility -
CORRECT ANSWER B
Which of the following errors would cause the most severe reaction?
A. Group A blood given to group O patient
B. Group O blood given to group A patient
C. Group B blood given to group AB patient
D. Group O blood given to group B patient - CORRECT ANSWER A
All of the following are part of the preliminary evaluation of a transfusion reaction
except:
A. Check pre and post transfusion samples for the color of the serum.
B. Perform ABO and Rh recheck
C. DAT on the post transfusion reaction sample
D. Panel on pre and post transfusion sample - CORRECT ANSWER D
All of the following test should be done if a hemolytic transfusion has taken place
except:
newest edition 2025//complete with
questions and correct answers
What is the disposition of a donor unit that contains an antibody?
A. The unit must be discarded.
B. Only the plasma can be used to make components.
C. The antibody must be absorbed from the unit.
D. Label the unit indicating that it contains antibody; release into inventory. - CORRECT
ANSWER D
Given situation where cells, crosshatch, auto control and DAT (anti-IgG) are all positive,
what procedure should be performed next?
A. Absorption using rabbit stroma
B. Antigen type the patients' cells
C. Elution followed by a cell panel on the elute
Decal panel - CORRECT ANSWER C
Can Rh positive blood can ever be transfused to a Rh negative person?
A. Never; under no circumstances
B. Only if Rh positive have been previously administered
C. May be transfused to males or females past childbearing age
D. Depends on the responder status of the individual - CORRECT ANSWER C
A crosshatch and screen cells are 2+ at immediate spin, 1+ at 37 C, and negative at the
IAT phase. Identify the problem.
A. Combination of antibodies
B. Cold antibodies
C. Rouleaux
D. Test error - CORRECT ANSWER B
What corrective action should be taken when rouleaux causes positive test results?
A. Wash cells and dilute serum
B. Perform an auto absorption
C. Run a panel
D. Perform an elution - CORRECT ANSWER A
,All of the following are the reasons for performing an absorption except:
A. Separation of mixtures of antibodies
B. Removal of interfering antibodies
C. Confirmation of weak antigen on red cells
D. Identification of antibodies causing positive DAT - CORRECT ANSWER D
What is needed for detection of some drug-induced antibodies?
A. Panel cells
B. Polyspecific Coombs sera
C. Enzyme-treated cells
D. Drug-coated cells - CORRECT ANSWER D
How can IgG antibodies be removed from red cells?
A. ZZAP
B. Cold elution
C. Thiol reagent
D. AET - CORRECT ANSWER A
How can IgM antibodies be separated from IgG antibodies?
A. Warm elution
B. Thiol reagents
C. Absorption with antigen-specific red cells
D. Impossible to separate - CORRECT ANSWER B
All of the condition below would require elution to identify antibodies coating the red
cells EXCEPT:
A. HDN
B. Hemolytic anemia
C. Multiple Myeloma
D. Transfusion reaction - CORRECT ANSWER C
In the IAT phase, what action should be taken if tubes are negative after addition of
check cells (reagent sensitized cells)?
A. Repeat the test; replace AHG reagent and check the cell washer.
B. Add additional check cells
C. Add additional AHG reagent
D. Record results as negative - CORRECT ANSWER All of the following would be used
to test the reactivity of AHG reagent except:
A. Diluted anti-K antisera
, B. Diluted anti-D antisera
C. Check cells
D. 6% albumin - CORRECT ANSWER A
What is the first step in the laboratory investigation of a transfusion reaction?
A. DAT on the post-transfusion sample
B. Check for clerical error
C. Repeat ABO and Rh typing of patient and donor unit.
D. Antibody screen on the post-transfusion sample. - CORRECT ANSWER B
A Trauma patient had a severe hemolytic reaction following a blood transfusion. What is
the most likely cause?
A. Immediate, no immunological: probably due to volume overload
B. Delayed Immunological: probably due to an antibody such as anti-Jk^a
C. Delayed no immunological: probably due to iron overload
D. Immediate, no immunological: probably due to clerical error, ABO incompatibility -
CORRECT ANSWER D
A patient has hemolytic reaction transfused 8 days ago. What is the most likely cause?
A. Immediate, no immunological: probably due to volume overload
B. Delayed Immunological: probably due to an antibody such as anti-Jk^a
C. Delayed no immunological: probably due to iron overload
D. Immediate, no immunological: probably due to clerical error, ABO incompatibility -
CORRECT ANSWER B
Which of the following errors would cause the most severe reaction?
A. Group A blood given to group O patient
B. Group O blood given to group A patient
C. Group B blood given to group AB patient
D. Group O blood given to group B patient - CORRECT ANSWER A
All of the following are part of the preliminary evaluation of a transfusion reaction
except:
A. Check pre and post transfusion samples for the color of the serum.
B. Perform ABO and Rh recheck
C. DAT on the post transfusion reaction sample
D. Panel on pre and post transfusion sample - CORRECT ANSWER D
All of the following test should be done if a hemolytic transfusion has taken place
except:
