Lecture 4
Genetic approach of altering protein
,Lecture 4- Intended Learning Outcomes
• Investigate how desired mutations can be introduced into a cloned
gene.
• Explain how these mutations are used to introduce desired proper
in a protein.
,• Mutation- A change in the nucleic acid sequence (bases) of genetic
material in an organism.
Mutations
Point and small mutations large mutations
• Silent Nucleic acid changes within a la
chromosomal region
• Missense
• Nonsense
• Frameshift by additions
• Frameshift by deletions
, Mutagenesis
• Why modify genes? Why not proteins?
• The process of generating amino acid coding changes at the DNA le
is called mutagenesis.
- Non-specific (random)
- Specific (site-directed)
Genetic approach of altering protein
,Lecture 4- Intended Learning Outcomes
• Investigate how desired mutations can be introduced into a cloned
gene.
• Explain how these mutations are used to introduce desired proper
in a protein.
,• Mutation- A change in the nucleic acid sequence (bases) of genetic
material in an organism.
Mutations
Point and small mutations large mutations
• Silent Nucleic acid changes within a la
chromosomal region
• Missense
• Nonsense
• Frameshift by additions
• Frameshift by deletions
, Mutagenesis
• Why modify genes? Why not proteins?
• The process of generating amino acid coding changes at the DNA le
is called mutagenesis.
- Non-specific (random)
- Specific (site-directed)
