Summary - Advanced Immunology MED-BMS88 (MED-BMS88)

SSA Novel Immune cells and concept
Learning aims:

You can name the origin, key characteristics and function of Innate
Lymphoid cell (ILC) subsets, CD4+ T-helper cell subsets and dendritic cell
subsets, which you can use to identify them in an experiment and
interpret their reaction in different immunological situations.

Mindmap: CD4+ T cells

Function Phenotypic Transcription Induced Produce
al by (cytokines)
Characteristics
characteristi
cs

Th1 ∙ Support CXCR3+ Tbet+ IFNgamm TNFalfa, IFNgam
macrophages against (intracellular) a, IL12 ma, GMCSF, IL2
CD3+, CD4+
IC pathogen

Th2 ∙ Against parasite CRTH2+ GATA3+ IL4 TGFbeta, IL10,
infections IL4, IL5, IL13
CD3+, CD4+
∙ Support eosinophils,
CCR4
basophils, mast cells

∙ Support B cells

Th17 ∙ Activate epithelium CCR6+ RORyt+ TGFbeta, IL17, IL21, IL22,
IL6, IL21, IL26
∙ Activate neutrophils CD3+, CD4+
IL23
∙ Defense against (CCR4, CD161)
extracellular pathogen IL17
(fungi)

Treg ∙ Support other CD3+, FOXp3+ TGFbeta IL35, TGFbeta,
immune cells (T cells) CD4+, CD25+, IL10
(STAT5)
CD127-, IL2Ra
∙ Prevent auto
immunity (PD1, CD152,
CD25, CD127)
∙ Promote cancer
(checkpoint inhibition
therapy)

Th9 ∙ Defense against CD3+, CD4+, IRF4, PU.1 IL2, IL9
extra cellular TGFbeta,
CCR6, CCR3,
parasites IL4
CXCR3
∙ Cancer regulation

∙ Mucosal
autoimmunity

,Tfh ∙ Support B cell in CD3+, CD4+, BCL6 IL23, IFNgamma, IL21,
increase antibody CXCR5 (binding IL16, IL4
affinity enables Tfh to TGFbeta
leave T cell
∙ Support B cell in
areas of the
activation
second
∙ Support B cells in lymphoid tissue
isotype switch and move the
follicles in B cell
areas



Hematopoiesis of T cells and Innate Lymphoid cells




∙ Th9 arises from CD4+ T cells

∙ Tfh (T follicular helper cell) arises from lymphoid progenitor, Effector CD4
T cell that is present in lymphoid follicles that cooperates with B cells,
helping them to produce antibody

Mindmap: DC cells

Function Phenotypic Transcription Hematopoiesis Induced Produce
al by (cytokin
Characterist
characteristic es)
ics
s

pDC ∙ React to C45RA, CD12 Common Myeloid TLR7, TNFalfa,
virus/tumor 3, CD303 Progenitor > TLR9 IFNalfa,
(BDCA2), CD Granulocyte- (recognit IL6,
304 macrophage DC ion Granzym
(BDCA4) progenitor (GMDP) > receptor) e B
Macrophage DC
No
progenitor (MDP) >
expression: C
Common DC progenitor
D11c, CD33,
(CDP)
CD11b, CD13

cDC ∙ React to CLEC9A, IRF3, IRF5, Common Myeloid TLR3/9/2 CXCL9/1
1 intracellular CD33, CD141 IRF7, NF- Progenitor > 0, Th1, 0, IL12,

, pathogens , CD13, kappaB Granulocyte- NK cell TNFalfa
macrophage DC (activati
low CD11c,
progenitor (GMDP) > on of
low CD11b Macrophage DC CD8+
progenitor (MDP) > cells)
Common DC progenitor
(CDP) >

Pre-DC

cDC ∙ React to CD33, CD11c Common Myeloid TLR4/5/2 IL10,
2 extracellular , SIRPalfa, C Progenitor > &6 IL12,
bacteria/fun D1c, CD2, Granulocyte- IL23,
gi CD11b, CD13 macrophage DC TNFalfa
progenitor (GMDP) >
∙ to
Macrophage DC
intracellular
progenitor (MDP) >
pathogen
Common DC progenitor
∙ to (CDP) >
parasites
Pre-DC
and
allergens



Mindmap: Innate lymphoid cells

Function Phenotypic Transcriptio Hematopoie Induced Produce
nal sis by (cytokine
Characteristi
characteristi s)
cs
cs

Natur ∙ React to IC CD56+, EOMES Common IL7, IL15 IFNgamma
al pathogens CD16+ lymphoid ,
Killer (mimic CD8+ progenitor granzymes
CD3-
(NK) cells) (CLP) > , perforins
cells
∙ Virus and Common
tumor cells Innate
Lymphoid
∙ cytotoxicity
progenitors
(CILP) >

NK progenitor

ILC1 ∙ React to IC CD3-, CD49a, NFIL3, Tbet, Common IFNgamm Granzymes
pathogens TRAIL, RUNX3, lymphoid a, , Perforin,
(mimic Th1 NKp46, CD127 progenitor TNFalfa, IFNgamma
cells) +, CD117+ (CLP) > activated
by
∙ Virus and Common
cytokines
tumor cells Innate
Lymphoid
progenitors