1. Zaccahrias and Hans Janssen: produced the compound microscope
2. Antony Van Leeuwenhoek: father of microscopy, first to observe live bacteria
and protozoans or animacules.
3. Robert Hooke: published Microphagia. Improved the compound light
microscope.
4. Simple Light Microscope: single magnifying lens and uses visible light to
illuminate.
5. Compound Light Microscope: multiple lenses and uses light from electric
source to illuminate.
6. Ocular lens: lenses close to the eye
7. Objective lens: lenses closer to the specimen and in the body of the
microscope
8. Magnifying power: Ocular lens power multiplied by the objective lens power.
Ocular is generally 10 times
9. Bright field microscope: background is brighter than specimen; specimens
must be stained
10. Dark field microscope: unfixed and unstained specimens, specimen
appears bright due to dark background, spirochetes and bacterial capsules are
observed
11. Types of compound microscopes: dark and bright field, phase contrast,
fluorescence, confocal, electron, TEM and SEM microscopes.
12. Phase Contrast Microscope: produce high contrast images, ideal for
cytoplasmic streaming, motility and dynamic states of cell organelles.
13. Fluorescence Microscope: ultraviolet illumination, visualize specimen with
natural fluorescence like chlorophyl or that have been stained with
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