Microbio Lab Midterm Study Guide Question and answers
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1. What is
Eliminating any possibility of intervention of foreign bacteria (contamination)
aseptic
into the media.
technique?
It is necessary since we are working with live bacteria samples and trying to
2. Why is aseptic
technique define the unknown bacteria.
neces- sary for
this lab?
It is used when preparing, opening, and transferring bacterial samples to
3. When should any
aseptic technique sterile media.
be used?
4. What are the 7. Where and how do you steril-
proper order ize an inoculating
of steps
needed to
perform an
asep- tic
transfer?
5. A Bunsen
burner should
have how
many cones?
6. Where is the
hottest part of
an active
Bunsen
burner?
,Microbio Lab Midterm Study Guide Question and answers
rated A+ 2025
1. Clean & disinfect
counter
2. Light bunsen burner
3. Keep all media and
equipment near the flame
3. Flame inoculating
loop/needle from the
handle to the tip
4. Pass the lip of tubes
with bacterial or sterile
media through the flame
before and after inoculating
it.
5. Use agar plate lids as
a shield
6. No talking during
procedure
2, a light (outer) and dark
(inner) blue cone
The tip of the inner dark
blue cone
Flame inoculating
loop/needle from the handle
to the tip, passing it through
the hottest part of flame.
, Microbio Lab Midterm Study Guide Question and answers
rated A+ 2025
tool (loop) or Pass the lip of tubes with bacterial or sterile media through the flame
a glass before and after inoculating it.
tube?
8. What is a culture? A culture is a population of bacteria grown in the laboratory.
Define a pure and A pure culture contains only one single type.
mixed culture. A mixed culture contains two or more ditterent bacteria types.
9. What is a A colony refers to the individual colonies of bacteria growing together.
colony in
Microbiology? CFU stands for colony-forming unit. It's a unit used to estimate the
Define a CFU. number of viable bacteria cells in a sample. (able to count on the
plate)
10. What is the
Obtaining isolation of bacterial species from a sample
goal of all
streak pat-
terns?
1. Sterilize loop
11. What are the
steps to
2. Grab sample
perform a
quadrant and with loop 3.
zigzag
-For Quadrant Streak:
streak?
Streak first quad then flame loop before streaking the next quad. (flame in
between streaking each quad)
-For Zigzag Streak:
Streak the entire plate in a zigzag pattern one time.
12. When would each Zigzag Streaking is used to isolate colonies from samples which may
be of low cell
streak pattern bedensity.
used?
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1. What is
Eliminating any possibility of intervention of foreign bacteria (contamination)
aseptic
into the media.
technique?
It is necessary since we are working with live bacteria samples and trying to
2. Why is aseptic
technique define the unknown bacteria.
neces- sary for
this lab?
It is used when preparing, opening, and transferring bacterial samples to
3. When should any
aseptic technique sterile media.
be used?
4. What are the 7. Where and how do you steril-
proper order ize an inoculating
of steps
needed to
perform an
asep- tic
transfer?
5. A Bunsen
burner should
have how
many cones?
6. Where is the
hottest part of
an active
Bunsen
burner?
,Microbio Lab Midterm Study Guide Question and answers
rated A+ 2025
1. Clean & disinfect
counter
2. Light bunsen burner
3. Keep all media and
equipment near the flame
3. Flame inoculating
loop/needle from the
handle to the tip
4. Pass the lip of tubes
with bacterial or sterile
media through the flame
before and after inoculating
it.
5. Use agar plate lids as
a shield
6. No talking during
procedure
2, a light (outer) and dark
(inner) blue cone
The tip of the inner dark
blue cone
Flame inoculating
loop/needle from the handle
to the tip, passing it through
the hottest part of flame.
, Microbio Lab Midterm Study Guide Question and answers
rated A+ 2025
tool (loop) or Pass the lip of tubes with bacterial or sterile media through the flame
a glass before and after inoculating it.
tube?
8. What is a culture? A culture is a population of bacteria grown in the laboratory.
Define a pure and A pure culture contains only one single type.
mixed culture. A mixed culture contains two or more ditterent bacteria types.
9. What is a A colony refers to the individual colonies of bacteria growing together.
colony in
Microbiology? CFU stands for colony-forming unit. It's a unit used to estimate the
Define a CFU. number of viable bacteria cells in a sample. (able to count on the
plate)
10. What is the
Obtaining isolation of bacterial species from a sample
goal of all
streak pat-
terns?
1. Sterilize loop
11. What are the
steps to
2. Grab sample
perform a
quadrant and with loop 3.
zigzag
-For Quadrant Streak:
streak?
Streak first quad then flame loop before streaking the next quad. (flame in
between streaking each quad)
-For Zigzag Streak:
Streak the entire plate in a zigzag pattern one time.
12. When would each Zigzag Streaking is used to isolate colonies from samples which may
be of low cell
streak pattern bedensity.
used?
