with Complete Answers Rated A+
2025-2026.
Anton van Leeuwenhoek - Answer Father of microbiology
Created 1st magnifying glass
Coined the term 'Animalcules'
Animalcules - Answer First term used to describe microorganisms
What is the theory of Spontaneous Generation? - Answer Life arises spontaneously from non-
living material (abiogenesis)
Who were the scientists involved in the theory of spontaneous generation? - Answer
Francesco Redi (meat in jars experiment)
Louis Pasteur (Swan Neck Flask experiment)
John Tyndall (found heat resistant microbes)
Ferdinand Cohn (discovered endospores)
Robert Koch (discovered anthrax disease by spore forming bacteria)
What were the structures present in hay infusions? - Answer Heat resistant microbes called
endospores
Which experiment disproved TSG (theory of spontaneous generation)? - Answer Louis Pasteur
swan neck flask experiment
What is bioremediation? - Answer The use of microorganisms to clean pollution
How do viruses grow? - Answer They cannot reproduce on their own. The must use a living
host cell
What does the resolving power of a microscope do? - Answer Distinguish two objects that are
close together
, Which microscope is used to observe a specimen at the atomic level? - Answer Atomic Force
Microscope (1980)
What does positive staining do? - Answer Contains positive charge that dyes the cells
What does negative staining do? - Answer Contains negative charge that dyes the background
Which dyes contain a positive charge? - Answer Basic dyes
Which dyes contain a negative charge? - Answer Acidic dyes
What is the advantage of the size of prokaryotes? - Answer Their small size makes it so they
have a rapid growth rate
Steps used for a gram stain:
1: Crystal Violet 2: Iodine 3: Alcohol 4: Safranin
A: 1,3,4,2
B: 1,3,2,4
C: 2,3,1,4
D: 1,2,3,4 - Answer D
Which step during the process of gram staining could cause for a gram+ stain to appear to be a
gram- stain? - Answer Step 3, decolorization using alcohol
Why is it important to study microorganisms/bacteria? - Answer We can not live without them
What is the equation to find the total magnification from a microscope? - Answer Objective
lens x Ocular lens
Diplococcus - Answer pairs
Streptococcus - Answer chains