MLT ASCP BOARD OF CERTIFICATION
EXAM | NEWEST ACTUAL EXAM
COMPREHENSIVE QUESTIONS AND
VERIFIED ANSWERS GRADED A+ | 100%
PASS | 2025 UPDATE!
The principle behind the diagnosis of Paroxysmal Nocturnal
Hemoglobinuria (PNH) by flow cytometry is: - ✔✔✔ Correct Answer
> Using a fluorescein labeled proaerolysin variant to selectively bind to
GPI anchor proteins
An adult patient presents with a temperature of 103°F (39.4°C). A CBC
is ordered and the white blood cell (WBC) count is 15.0 x 109/L
(reference interval= 4.0 -11.0 x 109/L) with 10% band neutrophils and
2% metamyelocytes. What can be concluded at this point with these
clinical and laboratory results? - ✔✔✔ Correct Answer > This is most
likely a bacterial infection or inflammatory response.
This most likely is either a bacterial infection or systemic inflammatory
response. Increased temperature, increased white blood count, and
immature cells of the neutrophilic line are all indicators of such.
,Symptoms and CBC results can vary in viral infections. However, a
response by the lymphocytes is much more likely than by the
neutrophils.
Normal fecal specimens contain all of the following, EXCEPT: - ✔✔✔
Correct Answer > Shigella
What are the usual results of the mean corpuscular volume (MCV) and
the red cell distribution width (RDW) in alpha thalassemia? - ✔✔✔
Correct Answer > The MCV is decreased but the RDW is usually
increased
A patient is seen at his physician's office for scaling, flat lesions on his
back that are irregular with white blotches of discoloration (top image).
The bottom image shown is of a tease mount taken from the skin and
stained with Periodic Acid Schiff, which reveals narrow fungal hyphae
(arrows) along with 3 - 5 µm in diameter, irregular sized spherical
budding yeast cells, a presentation often referred to as "spaghetti and
meatballs". Which fungi is the most likely cause of infection? - ✔✔✔
Correct Answer > Malassezia furfur
Which of the following statements is true regarding the staining
protocol used for bone marrow smears compared to that for peripheral
blood smear? - ✔✔✔ Correct Answer > Longer staining time should
be used to allow thorough staining.
,Bone marrow smears are much more cellular and require additional
contact time in both stain and buffer. Laboratories use longer staining
time to compensate for the relative thickness of marrow smears
compared to peripheral blood smears.
Which of the following inclusions are composed of DNA? - ✔✔✔
Correct Answer > Howell-Jolly bodies
A 40-year-old female receives two units of Red Blood Cells during a
surgical procedure. The patient has no prior history of transfusions.
Seven days later, she presents with extensive bruising of the
extremities and bleeding of the gums, with no additional symptoms.
Her platelet count is 5 x 109/L (reference interval 150 - 400 x 109/L).
What is the most likely diagnosis? - ✔✔✔ Correct Answer > Post
transfusion purpura (PTP)
Her symptoms, including severe thrombocytopenia one week after
transfusion, are most consistent with post-transfusion purpura (PTP).
PTP results as an anamnestic response to a platelet antigen. PTP
presents 1 to 24 days after a transfusion.
Each of the cells below can be found in all types of body fluids EXCEPT:
- ✔✔✔ Correct Answer > Mesothelial cells
Mesothelial cells are found only in serous body fluids including pleural
fluid, peritoneal fluid, and pericardial fluid.
, During data analysis in flow cytometry, how are cells gated? - ✔✔✔
Correct Answer > By placing a gate around cells with the same light-
scattering.
In flow cytometry, gating is the process of isolating cells with the same
light-scattering or fluorescence properties by electronically placing a
gate around them.
If a patient has a white blood count of 40,000/µL, what would be the
MOST useful staining procedure to distinguish between bacterial
infection and chronic myelogenous leukemia (CML)? - ✔✔✔ Correct
Answer > Leukocyte alkaline phosphatase (LAP)
The LAP stain is used to determine if an increase of cells is due to
chronic myelogenous leukemia or a leukemoid reaction due to infection
or similar conditions.
Raynaud's phenomenon is associated with a deficiency/dysfunction of
the complement fraction: - ✔✔✔ Correct Answer > C7
A decreased level of the complement fraction, C7, is associated with
Raynaud's disease, sclerodactyly, telangiectasia, and several bacterial
infections caused by Neisseria spp. Raynaud's phenomenon is a
condition of episodic constriction of small arteries of the extremities,
EXAM | NEWEST ACTUAL EXAM
COMPREHENSIVE QUESTIONS AND
VERIFIED ANSWERS GRADED A+ | 100%
PASS | 2025 UPDATE!
The principle behind the diagnosis of Paroxysmal Nocturnal
Hemoglobinuria (PNH) by flow cytometry is: - ✔✔✔ Correct Answer
> Using a fluorescein labeled proaerolysin variant to selectively bind to
GPI anchor proteins
An adult patient presents with a temperature of 103°F (39.4°C). A CBC
is ordered and the white blood cell (WBC) count is 15.0 x 109/L
(reference interval= 4.0 -11.0 x 109/L) with 10% band neutrophils and
2% metamyelocytes. What can be concluded at this point with these
clinical and laboratory results? - ✔✔✔ Correct Answer > This is most
likely a bacterial infection or inflammatory response.
This most likely is either a bacterial infection or systemic inflammatory
response. Increased temperature, increased white blood count, and
immature cells of the neutrophilic line are all indicators of such.
,Symptoms and CBC results can vary in viral infections. However, a
response by the lymphocytes is much more likely than by the
neutrophils.
Normal fecal specimens contain all of the following, EXCEPT: - ✔✔✔
Correct Answer > Shigella
What are the usual results of the mean corpuscular volume (MCV) and
the red cell distribution width (RDW) in alpha thalassemia? - ✔✔✔
Correct Answer > The MCV is decreased but the RDW is usually
increased
A patient is seen at his physician's office for scaling, flat lesions on his
back that are irregular with white blotches of discoloration (top image).
The bottom image shown is of a tease mount taken from the skin and
stained with Periodic Acid Schiff, which reveals narrow fungal hyphae
(arrows) along with 3 - 5 µm in diameter, irregular sized spherical
budding yeast cells, a presentation often referred to as "spaghetti and
meatballs". Which fungi is the most likely cause of infection? - ✔✔✔
Correct Answer > Malassezia furfur
Which of the following statements is true regarding the staining
protocol used for bone marrow smears compared to that for peripheral
blood smear? - ✔✔✔ Correct Answer > Longer staining time should
be used to allow thorough staining.
,Bone marrow smears are much more cellular and require additional
contact time in both stain and buffer. Laboratories use longer staining
time to compensate for the relative thickness of marrow smears
compared to peripheral blood smears.
Which of the following inclusions are composed of DNA? - ✔✔✔
Correct Answer > Howell-Jolly bodies
A 40-year-old female receives two units of Red Blood Cells during a
surgical procedure. The patient has no prior history of transfusions.
Seven days later, she presents with extensive bruising of the
extremities and bleeding of the gums, with no additional symptoms.
Her platelet count is 5 x 109/L (reference interval 150 - 400 x 109/L).
What is the most likely diagnosis? - ✔✔✔ Correct Answer > Post
transfusion purpura (PTP)
Her symptoms, including severe thrombocytopenia one week after
transfusion, are most consistent with post-transfusion purpura (PTP).
PTP results as an anamnestic response to a platelet antigen. PTP
presents 1 to 24 days after a transfusion.
Each of the cells below can be found in all types of body fluids EXCEPT:
- ✔✔✔ Correct Answer > Mesothelial cells
Mesothelial cells are found only in serous body fluids including pleural
fluid, peritoneal fluid, and pericardial fluid.
, During data analysis in flow cytometry, how are cells gated? - ✔✔✔
Correct Answer > By placing a gate around cells with the same light-
scattering.
In flow cytometry, gating is the process of isolating cells with the same
light-scattering or fluorescence properties by electronically placing a
gate around them.
If a patient has a white blood count of 40,000/µL, what would be the
MOST useful staining procedure to distinguish between bacterial
infection and chronic myelogenous leukemia (CML)? - ✔✔✔ Correct
Answer > Leukocyte alkaline phosphatase (LAP)
The LAP stain is used to determine if an increase of cells is due to
chronic myelogenous leukemia or a leukemoid reaction due to infection
or similar conditions.
Raynaud's phenomenon is associated with a deficiency/dysfunction of
the complement fraction: - ✔✔✔ Correct Answer > C7
A decreased level of the complement fraction, C7, is associated with
Raynaud's disease, sclerodactyly, telangiectasia, and several bacterial
infections caused by Neisseria spp. Raynaud's phenomenon is a
condition of episodic constriction of small arteries of the extremities,
