BIOL 3110 Final Exam NEWEST ACTUAL EXAM
BIOL 3110 FINAL EXAM NEWEST ACTUAL EXAM COMPLETE 300
QUESTIONS AND CORRECT DETAILED ANSWERS (VERIFIED ANSWERS)
|ALREADY GRADED A+||BRAND NEW VERSION!!
Which of the following mutations in the cis-elements of the lac operon will be
associated with the HIGHEST level of transcription, when E. coli is grown in the
presence of lactose but not glucose?
a. Mutation of the Repressor binding site so Repressor binds tighter.
b. Mutation of the translation initiation codon to a stop codon.
c. Mutation of the translation initiation codon to a Leu codon.
d. Mutation of the Catabolite activator protein (CAP) binding site so CAP does not
bind.
e. Mutation of the -35 region to change the sequence to match consensus.
e. Mutation of the -35 region to change the sequence to match consensus.
Which of the following statements about E. coli RNA polymerase is FALSE:
a. Binds DNA using the σ subunit.
b. Binds DNA using the α subunit.
c. Initiates RNA synthesis without a primer.
d. Uses the coding strand as a template.
e. Synthesizes RNA in the 5' to 3' direction.
d. Uses the coding strand as a template.
Which of the following statements about transcription in E. coli is TRUE:
a. Transcriptional regulation is coordinated by organizing protein coding regions
into operons.
b. Catabolite activator protein (CAP) links transcriptional regulation with glucose
levels in the growth media (environment).
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, BIOL 3110 Final Exam NEWEST ACTUAL EXAM
c. RNA polymerase transcribes all RNAs.
d. Promoter recognition is rate limiting.
e. All of the above.
e. All of the above.
Acetylation of the histone tail _________.
a. inactivates transcription of the target gene
b. enhances histone tail-DNA interactions
c. enhances histone tail-general transcription factor interactions
d. neutralizes the positive charge on histone
e. does not affect the efficiency of RNA polymerase recruitment
d. neutralizes the positive charge on histone
RNA Polymerase III:
a. Transcribes mRNA.
b. Transcribes the 28S RNA.
c. Transcribes tRNAs.
d. Does not require a promoter for initiation of transcription.
e. All of the above.
c. Transcribes tRNAs.
Which of the following best describes Tata binding protein (TBP):
a. Binds the Initiator element in RNA polymerase II promoters.
b. It is required for transcription initiation of RNA polymerase II.
c. Is required for double strand break repair.
d. It is part of the TFIIH complex.
e. Is responsible for transcriptional repression.
b. It is required for transcription initiation of RNA polymerase II.
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, BIOL 3110 Final Exam NEWEST ACTUAL EXAM
In eukaryotes, the following enzyme(s) play(s) a role in tRNA processing:
a. RNase P
b. CCA-adding enzyme
c. Endonuclease
d. Ligase
e. All of the above
e. All of the above
Which snRNP is required for branchpoint recognition:
a. the U1 snRNP
b. the U2 snRNP
c. the U6 snRNP
d. the 5.5S RNA
e. None of the above
b. the U2 snRNP
The carboxyl-terminal domain of the largest subunit of RNA polymerase II:
a. Contains the RNA polymerase II active site.
b. Undergoes acetylation during initiation.
c. Coordinates transcription with RNA processing.
d. Contains a repeat of basic amino acids that helps RNA polymerase II recognize
DNA.
e. Is not required for transcription of mRNA.
c. Coordinates transcription with RNA processing.
Cellular reverse transcriptase describes the enzymatic activity of which of the
following enzymes?
a. DNA polymerase.
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, BIOL 3110 Final Exam NEWEST ACTUAL EXAM
b. Exonuclease.
c. Telomerase.
d. Primase.
e. Topoisomerase.
c. Telomerase.
Which of the following activities is NOT shared by E. coli DNA polymerase I and
DNA polymerase III?
a. A proofreading activity.
b. A 5' to 3' polymerase activity.
c. A processive and fast rate of DNA synthesis.
d. A 5' to 3' exonuclease activity.
e. A 3' to 5' exonuclease activity.
d. A 5' to 3' exonuclease activity.
Which are the following statements BEST describes lagging strand synthesis in
DNA
replication?
a. Is coordinated with leading strand synthesis.
b. Involves DNA polymerization in the 5' to 3' direction.
c. Uses an RNA primer.
d. Requires DNA ligase.
e. All of the above are characteristics of lagging strand synthesis.
e. All of the above are characteristics of lagging strand synthesis.
In growing cells, unrepaired spontaneous deamination of cytosine is associated
with:
a. No change in DNA sequence because base pairing is unaltered.
b. A change in DNA sequence from a CG base pair to a TA base pair.
4|Page
BIOL 3110 FINAL EXAM NEWEST ACTUAL EXAM COMPLETE 300
QUESTIONS AND CORRECT DETAILED ANSWERS (VERIFIED ANSWERS)
|ALREADY GRADED A+||BRAND NEW VERSION!!
Which of the following mutations in the cis-elements of the lac operon will be
associated with the HIGHEST level of transcription, when E. coli is grown in the
presence of lactose but not glucose?
a. Mutation of the Repressor binding site so Repressor binds tighter.
b. Mutation of the translation initiation codon to a stop codon.
c. Mutation of the translation initiation codon to a Leu codon.
d. Mutation of the Catabolite activator protein (CAP) binding site so CAP does not
bind.
e. Mutation of the -35 region to change the sequence to match consensus.
e. Mutation of the -35 region to change the sequence to match consensus.
Which of the following statements about E. coli RNA polymerase is FALSE:
a. Binds DNA using the σ subunit.
b. Binds DNA using the α subunit.
c. Initiates RNA synthesis without a primer.
d. Uses the coding strand as a template.
e. Synthesizes RNA in the 5' to 3' direction.
d. Uses the coding strand as a template.
Which of the following statements about transcription in E. coli is TRUE:
a. Transcriptional regulation is coordinated by organizing protein coding regions
into operons.
b. Catabolite activator protein (CAP) links transcriptional regulation with glucose
levels in the growth media (environment).
1|Page
, BIOL 3110 Final Exam NEWEST ACTUAL EXAM
c. RNA polymerase transcribes all RNAs.
d. Promoter recognition is rate limiting.
e. All of the above.
e. All of the above.
Acetylation of the histone tail _________.
a. inactivates transcription of the target gene
b. enhances histone tail-DNA interactions
c. enhances histone tail-general transcription factor interactions
d. neutralizes the positive charge on histone
e. does not affect the efficiency of RNA polymerase recruitment
d. neutralizes the positive charge on histone
RNA Polymerase III:
a. Transcribes mRNA.
b. Transcribes the 28S RNA.
c. Transcribes tRNAs.
d. Does not require a promoter for initiation of transcription.
e. All of the above.
c. Transcribes tRNAs.
Which of the following best describes Tata binding protein (TBP):
a. Binds the Initiator element in RNA polymerase II promoters.
b. It is required for transcription initiation of RNA polymerase II.
c. Is required for double strand break repair.
d. It is part of the TFIIH complex.
e. Is responsible for transcriptional repression.
b. It is required for transcription initiation of RNA polymerase II.
2|Page
, BIOL 3110 Final Exam NEWEST ACTUAL EXAM
In eukaryotes, the following enzyme(s) play(s) a role in tRNA processing:
a. RNase P
b. CCA-adding enzyme
c. Endonuclease
d. Ligase
e. All of the above
e. All of the above
Which snRNP is required for branchpoint recognition:
a. the U1 snRNP
b. the U2 snRNP
c. the U6 snRNP
d. the 5.5S RNA
e. None of the above
b. the U2 snRNP
The carboxyl-terminal domain of the largest subunit of RNA polymerase II:
a. Contains the RNA polymerase II active site.
b. Undergoes acetylation during initiation.
c. Coordinates transcription with RNA processing.
d. Contains a repeat of basic amino acids that helps RNA polymerase II recognize
DNA.
e. Is not required for transcription of mRNA.
c. Coordinates transcription with RNA processing.
Cellular reverse transcriptase describes the enzymatic activity of which of the
following enzymes?
a. DNA polymerase.
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, BIOL 3110 Final Exam NEWEST ACTUAL EXAM
b. Exonuclease.
c. Telomerase.
d. Primase.
e. Topoisomerase.
c. Telomerase.
Which of the following activities is NOT shared by E. coli DNA polymerase I and
DNA polymerase III?
a. A proofreading activity.
b. A 5' to 3' polymerase activity.
c. A processive and fast rate of DNA synthesis.
d. A 5' to 3' exonuclease activity.
e. A 3' to 5' exonuclease activity.
d. A 5' to 3' exonuclease activity.
Which are the following statements BEST describes lagging strand synthesis in
DNA
replication?
a. Is coordinated with leading strand synthesis.
b. Involves DNA polymerization in the 5' to 3' direction.
c. Uses an RNA primer.
d. Requires DNA ligase.
e. All of the above are characteristics of lagging strand synthesis.
e. All of the above are characteristics of lagging strand synthesis.
In growing cells, unrepaired spontaneous deamination of cytosine is associated
with:
a. No change in DNA sequence because base pairing is unaltered.
b. A change in DNA sequence from a CG base pair to a TA base pair.
4|Page
