BMS 504 (latest update 2025-2026)
Practical Review Questions with Complete
Solutions {Grade A}
which type of bacteria grows strictly in the absence of oxygen?
a. obligate anaerobe
b. obligate aerobe
c. aerotolerant anaerobe
d. facultative anaerobe - correct answer a. obligate anaerobe
what are the three carbohydrates found in TSI agar slants?
a. glucose, sucrose ,lactose
b. fructose, sucrose, lactose
c. sucrose, glucose, maltose
d. lactose, maltose, sucrose - correct answer a. glucose, sucrose, lactose
A TSI slant graded K/A indicates a bacterium is capable of fermenting what
carbohydrates?
a. glucose
b. sucrose
c. fructose
d. all three of these sugars - correct answer a. glucose
TSI slants are inoculated by:
a. stabbing 2/3 into the media with pipet tip, pulling up, and fishtailing across the
slant
, BMS 504 (latest update 2025-2026)
Practical Review Questions with Complete
Solutions {Grade A}
b. stabbing 2/3 into the media with an inoculating needle, pulling up, and fishtailing
across the slant
c. stabbing 2/3 into the media with an inoculating loop, pulling up, and fishtailing
across the slant
d. stabbing all the way to the bottom of the media with an inoculating needle, pulling
up, and fishtailing across the slant - correct answer b. stabbing 2/3 into the media
with an inoculating needle, pulling up, and fishtailing across the slant
which of the following microorganisms is utilized to ferment grape juice into wine?
a. Serratia marcesceens
b. Clostridium sporogenes
c. Saccharomyces cerevisiae
d. Pseudomonas aeruginosa - correct answer c. Saccharomyces cerevisiae
T/F Escherichia coli will grow in anaerobic conditions - correct answer True
In which section of thioglycolate media would you expect to see an obligate
anaerobe growing?
a. only at the top of the media
b. evenly through the media
c. throughout the media, but more concentrated at the top
Practical Review Questions with Complete
Solutions {Grade A}
which type of bacteria grows strictly in the absence of oxygen?
a. obligate anaerobe
b. obligate aerobe
c. aerotolerant anaerobe
d. facultative anaerobe - correct answer a. obligate anaerobe
what are the three carbohydrates found in TSI agar slants?
a. glucose, sucrose ,lactose
b. fructose, sucrose, lactose
c. sucrose, glucose, maltose
d. lactose, maltose, sucrose - correct answer a. glucose, sucrose, lactose
A TSI slant graded K/A indicates a bacterium is capable of fermenting what
carbohydrates?
a. glucose
b. sucrose
c. fructose
d. all three of these sugars - correct answer a. glucose
TSI slants are inoculated by:
a. stabbing 2/3 into the media with pipet tip, pulling up, and fishtailing across the
slant
, BMS 504 (latest update 2025-2026)
Practical Review Questions with Complete
Solutions {Grade A}
b. stabbing 2/3 into the media with an inoculating needle, pulling up, and fishtailing
across the slant
c. stabbing 2/3 into the media with an inoculating loop, pulling up, and fishtailing
across the slant
d. stabbing all the way to the bottom of the media with an inoculating needle, pulling
up, and fishtailing across the slant - correct answer b. stabbing 2/3 into the media
with an inoculating needle, pulling up, and fishtailing across the slant
which of the following microorganisms is utilized to ferment grape juice into wine?
a. Serratia marcesceens
b. Clostridium sporogenes
c. Saccharomyces cerevisiae
d. Pseudomonas aeruginosa - correct answer c. Saccharomyces cerevisiae
T/F Escherichia coli will grow in anaerobic conditions - correct answer True
In which section of thioglycolate media would you expect to see an obligate
anaerobe growing?
a. only at the top of the media
b. evenly through the media
c. throughout the media, but more concentrated at the top
