AQA 74021
AA 1
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Exam (elaborations)
A-level BIOLOGY (7402/1) Paper 1 Mark scheme
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---16August 20222022/2023A+
- Question	Marking Guidance	Mark	Comments 
 
01.1	Any two of the following; Concentration of enzyme Volume of substrate solution 
pH	1	Allow same concentration of substrate 
 
01.2	Ratio between 4:1 and 5:1;;	2	Initial rates incorrect but correctly used = 1 mark 
 
01.3	At 60 ° C: 
1.	More kinetic energy; 
2.	More E–S complexes formed;	2	Allow converse for 37 ° C 
 
01.4	Different times: 
1.	Higher temperature / 60 ° C causes denaturation of all of enzyme; 
2.	Reaction stops (sooner) because ...
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