15MIBP0101

Gandhigram rural institute

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Protocol for isolation of mitochondria and SDS page
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december 2024

2024/2025
SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) Purpose: SDS-PAGE is a widely used technique for separating proteins based on their size. It is particularly useful in molecular biology and biochemistry for analyzing protein composition and verifying protein purity. Principle: Sodium dodecyl sulfate (SDS) is an anionic detergent that denatures proteins, coating them with a uniform negative charge. This allows proteins to be separated primarily by their size rather t...
mathes9842316610
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