Biochemistry
Hofstra University
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Thesis
Invitro Synthesis of Luciferase protein using rabbit reticulocyte cell expression system
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---13July 20222021/2022A
- This experiment explores the purification of T7 RNA polymerase and using this RNA polymerase to synthesize an RNA transcript followed by the in vitro synthesis of our protein of interest that is luciferase protein. The experiment helps to understand concepts of protein expression and purification through in-vitro transcription and translation. It is hypothesized 
 
that the purified T7 RNA polymerase will transcribe the linearized plasmid to luciferase mRNA transcript by in vitro transcription. ...
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