BIOPHYSICAL TECHNIQUE

JADAVPUR UNIVERSITY

Here are the best resources to pass BIOPHYSICAL TECHNIQUE. Find BIOPHYSICAL TECHNIQUE study guides, notes, assignments, and much more.

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Microscopy: Principles, Types, and Applications
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19

March 2026

2025/2026
This document provides a comprehensive overview of microscopy techniques. It begins with simple and compound microscopes, detailing their parts, magnification, and precautions. Phase contrast microscopy enables visualization of transparent specimens by converting phase shifts into contrast. Fluorescence microscopy uses fluorophores and filters to image specific structures in fixed and live cells. Polarization and dark-field microscopy are also covered for studying oriented structures and unstain...
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Analytical Techniques: Fluorimetry, Colorimetry, Spectroscopy, and Centrifugation
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17

March 2026

2025/2026
This document covers four key analytical and separation techniques. Fluorimetry is described based on fluorescence emission from excited molecules, with applications in environmental and food analysis. Colorimetry explains the Beer-Lambert law, instrumentation (light source, monochromator, cuvettes, detector), and uses in clinical and industrial settings. Spectroscopy introduces spectrometers, spectrophotometers, spectroscopes, and spectrographs for light-matter interaction studies. Centrifugati...
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Electrophoresis Techniques: Principles, Methods, and Applications
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10

March 2026

2025/2026
This document provides a comprehensive overview of electrophoresis, a technique for separating charged biomolecules like DNA, RNA, and proteins under an electric field. It covers the principle, factors affecting separation, and support media such as agarose and polyacrylamide gels. Various methods are detailed, including agarose gel electrophoresis, pulsed‑field gel electrophoresis (PFGE), immunoelectrophoresis, starch gel electrophoresis, isoelectric focusing, and Western blotting. Applicatio...
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X-ray Diffraction Data Analysis: From Crystal Symmetry to Structure Determination
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2

March 2026

2025/2026
This document details the process of X-ray diffraction data analysis for determining three-dimensional molecular structures. It explains how diffraction patterns are converted into electron density maps via Fourier transforms, beginning with indexing to identify unit cell dimensions and space group symmetry. Key steps include merging and scaling data, calculating R-factors, and addressing the phase problem through methods like molecular replacement, anomalous scattering, and heavy atom phasing. ...
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Overview of Spectroscopic Techniques: UV-Visible, Atomic Absorption, NMR, and EPR
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6

March 2026

2025/2026
This document details four key spectroscopy techniques. UV-Visible spectroscopy is explained via the Beer-Lambert law, covering instrumentation (light sources, monochromators) and applications like functional group detection. Atomic Absorption Spectroscopy is outlined for trace metal analysis, including its principle, advantages over flame photometry, and quantification methods. Nuclear Magnetic Resonance (NMR) spectroscopy covers the principle of nuclear spin, chemical shifts, and factors influ...
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Biophysics and Biophysical techniques
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16

March 2026

2025/2026
The documents provide a comprehensive overview of key biophysical and analytical techniques. They detail **chromatography** methods (column, ion-exchange, HPLC) for separation, **electrophoresis** (agarose, PAGE, PFGE) for charged molecule analysis, **spectroscopy** (UV-Vis, NMR, fluorescence) for structural and quantitative analysis, **microscopy** (phase contrast, electron) for imaging, and **X-ray diffraction** for 3D structure determination. These resources collectively explain the principle...
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