B23BT0401

Reva university

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Developmental Biology unit 3
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14

June 2025

2024/2025
Organogenesis CNS and epidermis –Neural crest cells – tissue Architecture of the Central Nervous System pinal cord and medulla organization Cerebellar organization Cerebral organization Adult neural stem cells The Somites and their derivatives – Development of tetrapod limb –Development of gonads– primary and secondary sex determination. Hormonal regulation of the sexual phenotype.
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Developmental Biology unit 1
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16

June 2025

2024/2025
Essential Concepts in Developmental Biology Early embryonic development in Frog, Gametogenesis, fertilization, cleavage, blastula and gastrula. Nuclear transplantation experiments in Amphibians and Acetabularia. Epigenesis and preformation Generating new cells and organs –– Cell-cell communication in development – Fate Maps. Switching genes on and off during development Ex. Differential expression of hemoglobin.
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Developmental Biology unit 4
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26

June 2025

2024/2025
Metamorphosis, Regeneration and Ageing Insect and amphibian metamorphosis. Regeneration in planaria and zebrafish – Biology of senescence, theories of aging and mechanism of aging – Eg. Role of mTOR pathway and telomeres in aging. Developmental Disorders: Genetic errors of human development, Developmental anomalies; Teratogenesis – types and mechanism of action.
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Biochemical Techniques unit 1
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15

June 2025

2024/2025
Precipitation: methods and applications. Centrifugation: Principle of centrifugation, types of centrifuges and rotors. Density gradient centrifugation and differential centrifugation -principle and applications. Dialysis-types, principle and applications. Electrophoresis: Principle, working procedure and applications of electrophoresis- Paper and gel electrophoresis (Agarose and SDS-PAGE).
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Biochemical Techniques unit 2: Chromatography
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12

June 2025

2024/2025
Unit 2: Chromatography: Classification and General Principles of chromatography, working procedure and applications of paper chromatography, thin layer chromatography, and affinity chromatography. Column chromatography–Principle, procedure and applications of Gel filtration, HPLC and GLC.
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Biochemical Techniques unit 3: Radio isotopic techniques
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54

June 2025

2024/2025
UNIT III 12 hrs Radio isotopic techniques: Atomic structure, radiation, type of radioactive decay, half0life, units of radio activity, detection and measurement of radio activity – methods based on ionization (GM- counter) application of isotope dilution technique. Biological hazards of radiation and safety measures in handling radio isotopes. Spectroscopic met...
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