Central Campus - Broward College

urease test purpose - Answer rapid or slow urease production urease test media - Answer phosphate buffer phenol red urea breaks down into - Answer ammonia and co2 urease test results - Answer positive: bright pink weak positive: pale pink negative: orange The ammonia produced causes the broth in a urease media tube to become - Answer alkaline and overcome a buffer added to the medium, resulting in a color change to bright pink by the pH indicator p...

Millimeter - Answer one thousandth of a meter micrometer - Answer millionth of a meter nanometer - Answer one billionth of a meter compound microscope - Answer - can magnify up to 1.000x - ocular and objective lenses phase-contrast microscope - Answer - amplifies the difference between refractive index (phase of light passing through specimens) of dense material and surrounding material. - makes cells and other materials appear darker fluorescent mi...

Where should you store your books in lab? - Answer In the cubby holes at the end of the bench Identify the part of the microscope that would move the specimen around while one was looking at it through the microscope. - Answer Stage control knob What would the total magnification be on our lab microscopes if the 40X objective lens were in place? - Answer 400X Identify a safety feature NOT present in our microbiology laboratory. - Answer Biosafety Level 4 isolatio...

Oil Immersion - Answer -Light bends/refracts when it moves from one medium to another -Only use with the 100x objective Resolution - Answer -The ability of a leans system to distinguish as separate entities objects that are very small and very close together -Determined by the quality of the lens and wavelength of light used Protozoa - Answer -Single-celled eukaryotic microorganisms -Classified by movement: flagella, cilia, amoeboid motion, and parasites with nonmot...

Innate resistance - Answer bodies first and second line of defense First line of defense - Answer to prevent pathogens entering the body, includes the skin and mucous membranes of respiratory, digestive, urinary, and reproductive systems, lacrimal apparatus, normal flora lacrimal apparatus function - Answer produces, distributes, and removes tears normal flora - Answer Microorganisms that reside in or on the body without causing disease Epidermis - Answer ...

metabolism - Answer the sum of all chemical reactions (anabolic and catabolic) within a living organism. OR and energy balance act anabolism - Answer (synthesis), chemical reactions in which energy is used to synthesize large molecules from simpler components Example: 1) Amino acid + Amino acid 2) capsule synthesis in bacteria dehydration synthesis - Answer removal of water to create a larger molecule catabolism - Answer (hydrolysis), the chemical breakdown o...

Who is credited with the discovery of bacteria using a magnifying glass as a microscope? - Answer Antony Van Leeuwenhoek Who is the scientist credited with the discovery of cells, particularly through the observation of a thin slice of cork, using an early microscope? - Answer Robert Hooke What is spontaneous generation, and how did it describe the belief about the origin of life from non-living material? - Answer spontaneous generation is the belief that life could arise...

Microorganisms - Answer organisms that are too small to be seen with the unaided eye Microbes in our life - Answer -pathogenic (disease-causing) -organic (carbon-based) (proteins, carbs, DNA) What do microbes produce? - Answer -produce industrial chemicals such as ethanol and acetone -produce fermented foods such as vinegar, cheese and bread -produce products in manufacturing (cellulase) and treatment (insulin) What are the 3 classifications in the ecosystem? - Answ...

coccus - Answer spherical bacillus - Answer rod shaped bacteria Spiral - Answer Spirilla What produces endospores? - Answer gram positive: *Bacillus* and *Clostridium* How does an endospore form? - Answer Each vegetative cell transforms into endospore during duress, each endospore germinates to form one vegetative cell. What is the process of endospore formation called? - Answer Sporulation; and the return of an endospore to its vegetative stat...

Robert Koch - Answer proved that bacteria can cause disease Robert Koch developed his postulates - Answer while identifying the cause of anthrax Developed the process of using just enough heat to kill bacteria that cause spoilage - Answer Pasteur Discovered the chemotherapeutic agent against syphilis - Answer Ehrlich Flemming - Answer discovered penicillin Pasteur - Answer discovered the cause of fermentation Lister is associated with - Answe...