Pellissippi State Community College

Pre Lab Assignment 3-PEA, MacConkey, & Morphological Unknown

1. What symptoms suggest that pertussis should be considered? Before she started coughing, she had a mild fever, runny nose, and sneezing for 10 days. According to the textbook, the beginning signs and symptoms of pertussis resemble those of a common cold—rhinorrhea, slight cough, and mild fever—if they occur at all. After one to two weeks, the signature symptom of severe coughing occurs. Vomiting, diarrhea, and choking can accompany this stage. To add to her symptoms, she did vomit on...

Assignment 1 Diseases of the Digestive System

Thioglycollate Exercise 1. What is Thioglycollate broth used for? Be specific!!! A. create oxygen gradient C. prevent growth B. keep temp constant D. grow colonies Aerotolance Exercise 2. Match the Following: b. anaerobe A. uses oxygen a. aerobe B. does NOT use oxygen Temperature Exercise 3. I was really worried when I found out that the organism I had just eaten was a ___ a. psychrophile c. xanthophile b. mesophile d. thermophile Exercise 6-2 – Urine Culture 4. Urine shoul

1. The Biol 2130 students and employees of a public school system developed diarrheal illness on April 2. The cafeteria served chicken that day. On April 1, part of the chicken was placed in water-filled pans and cooked in an oven for 2 hrs at a temp of 177oC. The oven was turned off and the chicken was left overnight in the warm oven. Symptoms of the disease included nausea, vomiting, cramping and a fever. All the patients recovered within 24 hrs. Two “serotypes” of a Gram positive, o...

BIOL 2130 Assignment 2-Diseases of the Respiratory System study guide spring 2023

Pre Lab Assignment 2-Staining

PEA 1. When examining a PEA plate that has been inoculated with organism and incubated for 24 hrs, we noticed that the organism is growing well. What does this tell us about the organism’s cell wall?  The organism’s cell wall was not inhibited by the phenylethyl alcohol. Also, the organism most likely has a thick peptidoglycan layer that causes the cell wall to stay intact; having a thick peptidoglycan layer would make the organism a Gram positive. 2. What is added to a PEA plate ...

PEA 1. When examining a PEA plate that has been inoculated with organism and incubated for 24 hrs, we noticed that the organism is growing well. What does this tell us about the organism’s cell wall?  The organism’s cell wall was not inhibited by the phenylethyl alcohol. Also, the organism most likely has a thick peptidoglycan layer that causes the cell wall to stay intact; having a thick peptidoglycan layer would make the organism a Gram positive. 2. What is added to a PEA plate ...

Exercise on Phenol Red Broth Analyze the test tubes blow and answer the following three questions: 1 2 3 4 5 1. The PR sucrose tube (#2) was yellow in color after inoculating them with an organism and incubating for 24 hrs. What is your analysis/interpretation of these results (yellow color)?  My analysis/interpretation is that there was fermentation with acid end products; also, there was no gas produced. 2. The PR sucrose tube (#5) was pink in color after inoculating them with an o...