BIOC0005

BIOC0005 - SECTION 3 BACTERIAL GENOMICS EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS VERIFIED LATEST UPDATE List out the 5 main uses of plasmids in the lab 1. Gene cloning (cloning vector, expression vector) 2. Gene editing/engineering (deliver CRISPR-Cas, gene knockout or knock in) 3. Reporter assay (reporter plasmids, fluorescent protein fusion) 4. Protein production and purification (expression plasmids, affinity tag plasmids) 5. Functional (gene overexpression, RNAi) ...

BIOC0005 - MOLECULAR BIOLOGY; PLASMIDS; PCR; OPERONS EXAM QUESTIONS AND ANSWERS WITH COMPLETE VERIFIED SOLUTIONS What is the centra dogma of molecular biology? A model that explain the flow of genetic information within a cell. Flow was originally considered to be unidirectional DNA → RNA → Protein. Reverse transcription disproves this. Can protein be the source of information flow? No - only recipient, DNA and RNA can be both. Who suggested the centra dogma? Francis Crick (1956) ...

BIOC0005 - BACTERIAL PLASMIDS AND CONJUGATION EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS VERIFIED LATEST UPDATE What is a plasmid? Non-chromosomal DNA Usually circular, but sometimes are linear Autonomous replication Stable inheritance What are some general differences between plasmids and chromosomes? Plasmids are generally smaller Plasmids are generally not essential for “normal” growth Plasmids are generally transferable What is the purpose of the origin of replication...

BIOC0005 - PROTEIN SYNTHESIS EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS VERIFIED What are the main phases of protein synthesis? initiation, elongation, termination Describe the regulatory regions and sequences in mRNAs. 7-methyl guanosine cap - prevent from degradation in the cytosol. Poly(A) tail - mRNA stability Start codon and stop codon (AUG, UGA) Describe the structures of tRNA that dictates it function. 3' end has the CCA sequence where amino acids are linked. The op...

BIOC0005 SECTION 2 EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS VERIFIED Which experiment showed that RNA polymerase binds to promoter in prokaryotes (-35 ~ -10)? DNAase footprinting experiment. Used chemical cleavage (type of DNA sequence) Which experiment showed that RNA pol was composed of multiple subunits Cellulose acetate chromatography showed RNA pol had sigma factor + 2 alpha + beta + beta' Describe the function of the RNA pol subunits sigma - binds to promoter region (-...

BIOC0005 - THE HUMAN GENOME EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS VERIFIED Describe the composition of the human nuclear genome. One genome has 23 chromosomes. 22 autosomes 50 to 260 Mb DNA 23 chromosomes has 3x10e9 bp, therefore somatic cells have 6x10e9. Chromosomes are arranged in order, with 1 being largest and 22 being smallest. Made up of 2 arms: p is the shorter arm and q is the longer one. Human genome encodes 21000 genes. Describe the proportions of sequences ...

BIOC0005 WEEK 15 EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS different types of post translatinal modifiations? - with disuphite bond formation, it happens when the cisteines residues are oxidated hence lose electron hence form a disulfite bond. this specific modification is what indicates to the cell that there is oxidative stress happening! - most cases tho, you have small molecules which are attached to the proetins eg: phosphates, methyl groups, acetyl groups, sugars, ubiquitin m...

BIOC0005 - PROTEIN TRAFFICKING EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS VERIFIED What are the two components of the cell? cytoplasm + nucleus What are the two components of the cytoplasm? cytosol + organelles How does a protein know where to be transported in a cell? Via sorting signals. This can be via signal sequences - exposed, single stretch of amino acids often at end of polypeptide chains. Or signal patches - amino acids contributing to the signal are separate until a...

BIOC0005 - DNA REPLICATION EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS DNA replication is... semiconservative How was the semiconservative nature of DNA replication demonstrated? Bacteria grown in 15N to make all DNA heavy, then transferred to 14N medium resulted in 15N-14N hybrid DNA, showing that the two strands of the double helix separate from each other during replication. Where does replication begin in prokaryotes? How does this compare to eukaryotes? Origin of replication...

MOLECULAR BIOLOGY - BIOC0005 EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS VERIFIED Describe the role of adenylyl cyclase in positive control of the lac operon Adenylyl cyclase catalyses the reaction of ATP to cAMP The IIA protein must be phosphorylated and bound to adenylyl cyclase for this to work The cAMP then goes on to bind with CAP to increase the transcription of lac operon Describe growth curve of E. coli grown with glucose AND lactose Diauxic growth so uses up glucose first...