BIOC0005

6 BIOC0005 EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS VERIFIED LATEST UPDATE who demonstrated semiconservative nature of DNA replication Meselson and Stahl Bacteria grown in 15N; all DNA is heavy Transfer to 14N medium Continued growth in 14N produce hybrid types of DNA -B form, right handed, helix turn every 3.4 nm and distance b/w two bases is 0.34 nm, 10 bp per turn most common -A form due to hight salt concentration or alcohol added, right handed, 2.3 nm makes a turn ...

BIOC0005 PART 2 EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS VERIFIED LATEST UPDATE Organelles Membrane-bound compartments in eukaryotic cells with specific functions Nuclear Localization Signal (NLS) Positively charged sequence on proteins for nuclear import Nuclear Pores Protein complexes allowing selective transport in and out of the nucleus Exportins Proteins facilitating protein export from the nucleus Mitochondrial Targeting Sequence (MTS) Sequence on proteins targeting t...

BIOC0005 PART 1 EXAM QUESTIONS AND ANSWERS WITH VERIFIED SOLUTIONS GRADED A++ Cloning vector DNA section allowing insertion of external DNA for cloning Plasmid vectors Artificial DNA molecules with replication origin, marker, insertion site Expression vectors Vectors allowing replication and expression of inserted DNA Promoter Region initiating gene transcription Terminator Sequence ending gene transcription Reporter genes Genes used to visualize regulatory elements' strength L...

BIOC0005 REVISION PACK EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS VERIFIED What are the different structures of DNA? A-DNA, B-DNA and Z-DNA What is the configuration of B-DNA? 10.5bp per turn of 3.32nm rise. 2nm in width possessing major & minor groves. What are A-DNA & Z-DNA? A-DNA is the dehydrated from of B-DNA. While Z-DNA is left-handed double helix What is a triple helix? When a 3rd strand of B-DNA is incorporated. What are the two extra proteinogenic amino acids? Selen...

5 BIOC0005 EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS VERIFIED LATEST UPDATE The Polymerase Chain Reaction (PCR) method for "cloning in vitro", as opposed to cloning using E. coli A simple and fast 'one-step' method for creating billions of copies of a target region of DNA Billions of copies Amplify by PCR PCR involves multiple cycles (up to ~35 cycles) of: 1- DNA denaturation @ 94oC - 98oC 2- Primers annealing to complementary DNA sequence @ ~ 50oC - 68oC 3- Elong...

BIOC0005 EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS VERIFIED LATEST UPDATE What is the order of the trp operon? trpL, trpE, trpD, trpC, trpB, trpA What is the trp operon controlled by? tryptophan concentration Where is trpa? a small sequence embedded in the leader sequence trpL What does the trp operon do? convert chorismic acid into tryptophan What happens to the trp operon when [trp] is high? operon is not transcribed trpR gene - to left of operon promoter, has own promote...

BIOC0005 EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS VERIFIED LATEST UPDATE Conjugation The transfer of genetic material (Plasmid or transposons) between cells by direct cell to cell contact What are some traits that can be required through Conjugation? Antibiotics Resistance, Heavy metal resistance, Pathogenicity islands (bacterial virulence), Metabolic Proteins What are the three most well known Gram-negative conjugation system? F-Plasmid, RP4, Ti Plasmid What are tra/trb The ...

BIOC0005 EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS VERIFIED LATEST UPDATE What is the function of alkaline phosphatase in DNA recombination? Alkaline phosphatase removes the 5' phosphate from the DNA to produce a 5' -OH meaning it is unable to form a phosphodiester bond and so a plasmid cannot religate during DNA recombination. Why are GC rich regions of DNA difficult to sequence? Because GC base pairs have 3 H-bonds which are difficult to separate. What is the function of ...